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Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches

Hui Xu, Zhen Li (UGent) , Peng‐Fei Jiang, Li Zhao, Chang Qu, Yves Van de Peer (UGent) , Yan‐Jing Liu and Qing‐Yin Zeng
(2022) PLANT JOURNAL. 110(1). p.129-146
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Abstract
Enzymes are essential components of all biological systems. The key characteristics of proteins functioning as enzymes are their substrate specificities and catalytic efficiencies. In plants, most genes encoding enzymes are members of large gene families. Within such families, the contribution of active site motif to the functional divergence of duplicate genes has not been well elucidated. In this study, we identified 41 glutaredoxin (GRX) genes in Populus trichocarpa genome. GRXs are ubiquitous enzymes in plants that play important roles in developmental and stress-tolerance processes. In poplar, GRX genes were divided into four classes based on clear differences in gene structure and expression pattern, subcellular localization, enzymatic activity, and substrate specificity of the encoded proteins. Using site-directed mutagenesis, this study revealed that the divergence of the active site motif among different classes of GRX proteins resulted in substrate switches and thus provided new insights into the molecular evolution of these important plant enzymes.
Keywords
Active site motif, Enzymatic specificity，Populus trichocarpa, Functional divergence, Gene family, Glutaredoxin, ARABIDOPSIS CHLOROPLASTIC GLUTAREDOXIN, GENE FAMILY, SUBCELLULAR RELOCALIZATION, FUNCTIONAL DIVERGENCE, CATALYTIC-PROPERTIES, YEAST GLUTAREDOXINS, ESCHERICHIA-COLI, SUPERGENE FAMILY, DUPLICATED GENES, REDOX REGULATION

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Citation

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MLA
Xu, Hui, et al. “Divergence of Active Site Motifs among Different Classes of Populus Glutaredoxins Results in Substrate Switches.” PLANT JOURNAL, vol. 110, no. 1, 2022, pp. 129–46, doi:10.1111/tpj.15660.
APA
Xu, H., Li, Z., Jiang, P., Zhao, L., Qu, C., Van de Peer, Y., … Zeng, Q. (2022). Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches. PLANT JOURNAL, 110(1), 129–146. https://doi.org/10.1111/tpj.15660
Chicago author-date
Xu, Hui, Zhen Li, Peng‐Fei Jiang, Li Zhao, Chang Qu, Yves Van de Peer, Yan‐Jing Liu, and Qing‐Yin Zeng. 2022. “Divergence of Active Site Motifs among Different Classes of Populus Glutaredoxins Results in Substrate Switches.” PLANT JOURNAL 110 (1): 129–46. https://doi.org/10.1111/tpj.15660.
Chicago author-date (all authors)
Xu, Hui, Zhen Li, Peng‐Fei Jiang, Li Zhao, Chang Qu, Yves Van de Peer, Yan‐Jing Liu, and Qing‐Yin Zeng. 2022. “Divergence of Active Site Motifs among Different Classes of Populus Glutaredoxins Results in Substrate Switches.” PLANT JOURNAL 110 (1): 129–146. doi:10.1111/tpj.15660.
Vancouver
1.
Xu H, Li Z, Jiang P, Zhao L, Qu C, Van de Peer Y, et al. Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches. PLANT JOURNAL. 2022;110(1):129–46.
IEEE
[1]
H. Xu et al., “Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches,” PLANT JOURNAL, vol. 110, no. 1, pp. 129–146, 2022.
@article{8733118,
  abstract     = {{Enzymes are essential components of all biological systems. The key characteristics of proteins functioning as enzymes are their substrate specificities and catalytic efficiencies. In plants, most genes encoding enzymes are members of large gene families. Within such families, the contribution of active site motif to the functional divergence of duplicate genes has not been well elucidated. In this study, we identified 41 glutaredoxin (GRX) genes in Populus trichocarpa genome. GRXs are ubiquitous enzymes in plants that play important roles in developmental and stress-tolerance processes. In poplar, GRX genes were divided into four classes based on clear differences in gene structure and expression pattern, subcellular localization, enzymatic activity, and substrate specificity of the encoded proteins. Using site-directed mutagenesis, this study revealed that the divergence of the active site motif among different classes of GRX proteins resulted in substrate switches and thus provided new insights into the molecular evolution of these important plant enzymes.}},
  author       = {{Xu, Hui and Li, Zhen and Jiang, Peng‐Fei and Zhao, Li and Qu, Chang and Van de Peer, Yves and Liu, Yan‐Jing and Zeng, Qing‐Yin}},
  issn         = {{0960-7412}},
  journal      = {{PLANT JOURNAL}},
  keywords     = {{Active site motif,Enzymatic specificity，Populus trichocarpa,Functional divergence,Gene family,Glutaredoxin,ARABIDOPSIS CHLOROPLASTIC GLUTAREDOXIN,GENE FAMILY,SUBCELLULAR RELOCALIZATION,FUNCTIONAL DIVERGENCE,CATALYTIC-PROPERTIES,YEAST GLUTAREDOXINS,ESCHERICHIA-COLI,SUPERGENE FAMILY,DUPLICATED GENES,REDOX REGULATION}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{129--146}},
  title        = {{Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches}},
  url          = {{http://doi.org/10.1111/tpj.15660}},
  volume       = {{110}},
  year         = {{2022}},
}
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