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Lung cell tropism and inflammatory cytokine-profile of porcine respiratory coronavirus infection

Kalina Atanasova (UGent) , S Van Gucht, Filip Barbé (UGent) , David Lefebvre (UGent) , Koen Chiers (UGent) and Kristien Van Reeth (UGent)
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Abstract
Knowledge about porcine respiratory coronavirus (PRCV) tropism was limited to morphological identification of target cells and controversial reports on replication in macrophages. This study aimed to clarify the lung cell tropism of a Belgian PRCV strain and to examine the lung profile of inflammatory cytokines for 15 days after intratracheal PRCV inoculation of gnotobiotic piglets. Until 5 days after inoculation, more than 50% of the PRCV-positive cells were type 2 pneumocytes, 30% bronchiolar epithelial cells, and 10% macrophages, as demonstrated by immunofluorescence stainings with specific cell markers. In vitro, PRCV productively infected primary porcine lung epithelial cells, but not porcine alveolar macrophages. Bronchoalveolar lavage fluids of PRCV-inoculated pigs contained high levels of interferons (IFN-α, IFN-γ) and interleukin-6, moderate interleukin-12 and low tumour necrosis factor-α levels. Our results indicate that PRCV infects primarily type 2 pneumocytes and induces lower lung cytokine levels, compared to swine influenza virus or lipopolysaccharide-complicated PRCV infections.
Keywords
type 2 pneumocytes, cytokines, PRCV, lungs, alveolar damage

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Chicago
Atanasova, Kalina, S Van Gucht, Filip Barbé, David Lefebvre, Koen Chiers, and Kristien Van Reeth. 2008. “Lung Cell Tropism and Inflammatory Cytokine-profile of Porcine Respiratory Coronavirus Infection.” Open Veterinary Science Journal 2: 117–126.
APA
Atanasova, K., Van Gucht, S., Barbé, F., Lefebvre, D., Chiers, K., & Van Reeth, K. (2008). Lung cell tropism and inflammatory cytokine-profile of porcine respiratory coronavirus infection. OPEN VETERINARY SCIENCE JOURNAL, 2, 117–126.
Vancouver
1.
Atanasova K, Van Gucht S, Barbé F, Lefebvre D, Chiers K, Van Reeth K. Lung cell tropism and inflammatory cytokine-profile of porcine respiratory coronavirus infection. OPEN VETERINARY SCIENCE JOURNAL. 2008;2:117–26.
MLA
Atanasova, Kalina, S Van Gucht, Filip Barbé, et al. “Lung Cell Tropism and Inflammatory Cytokine-profile of Porcine Respiratory Coronavirus Infection.” OPEN VETERINARY SCIENCE JOURNAL 2 (2008): 117–126. Print.
@article{873294,
  abstract     = {Knowledge about porcine respiratory coronavirus (PRCV) tropism was limited to morphological identification of target cells and controversial reports on replication in macrophages. This study aimed to clarify the lung cell tropism of a Belgian PRCV strain and to examine the lung profile of inflammatory cytokines for 15 days after intratracheal PRCV inoculation of gnotobiotic piglets. Until 5 days after inoculation, more than 50\% of the PRCV-positive cells were type 2 pneumocytes, 30\% bronchiolar epithelial cells, and 10\% macrophages, as demonstrated by immunofluorescence stainings with specific cell markers. In vitro, PRCV productively infected primary porcine lung epithelial cells, but not porcine alveolar macrophages. Bronchoalveolar lavage fluids of PRCV-inoculated pigs contained high levels of interferons (IFN-\ensuremath{\alpha}, IFN-\ensuremath{\gamma}) and interleukin-6, moderate interleukin-12 and low tumour necrosis factor-\ensuremath{\alpha} levels. Our results indicate that PRCV infects primarily type 2 pneumocytes and induces lower lung cytokine levels, compared to swine influenza virus or lipopolysaccharide-complicated PRCV infections.},
  author       = {Atanasova, Kalina and Van Gucht, S and Barb{\'e}, Filip and Lefebvre, David and Chiers, Koen and Van Reeth, Kristien},
  issn         = {1874-3188},
  journal      = {OPEN VETERINARY SCIENCE JOURNAL},
  language     = {eng},
  pages        = {117--126},
  title        = {Lung cell tropism and inflammatory cytokine-profile of porcine respiratory coronavirus infection},
  url          = {http://dx.doi.org/10.2174/1874318800802010117},
  volume       = {2},
  year         = {2008},
}

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