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Lung cell tropism and inflammatory cytokine-profile of porcine respiratory coronavirus infection

Kalina Atanasova UGent, S Van Gucht, Filip Barbé UGent, David Lefebvre UGent, Koen Chiers UGent and Kristien Van Reeth UGent (2008) OPEN VETERINARY SCIENCE JOURNAL. 2. p.117-126
abstract
Knowledge about porcine respiratory coronavirus (PRCV) tropism was limited to morphological identification of target cells and controversial reports on replication in macrophages. This study aimed to clarify the lung cell tropism of a Belgian PRCV strain and to examine the lung profile of inflammatory cytokines for 15 days after intratracheal PRCV inoculation of gnotobiotic piglets. Until 5 days after inoculation, more than 50% of the PRCV-positive cells were type 2 pneumocytes, 30% bronchiolar epithelial cells, and 10% macrophages, as demonstrated by immunofluorescence stainings with specific cell markers. In vitro, PRCV productively infected primary porcine lung epithelial cells, but not porcine alveolar macrophages. Bronchoalveolar lavage fluids of PRCV-inoculated pigs contained high levels of interferons (IFN-α, IFN-γ) and interleukin-6, moderate interleukin-12 and low tumour necrosis factor-α levels. Our results indicate that PRCV infects primarily type 2 pneumocytes and induces lower lung cytokine levels, compared to swine influenza virus or lipopolysaccharide-complicated PRCV infections.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
type 2 pneumocytes, cytokines, PRCV, lungs, alveolar damage
journal title
OPEN VETERINARY SCIENCE JOURNAL
Open Vet. Sci. J.
volume
2
pages
117 - 126
ISSN
1874-3188
DOI
10.2174/1874318800802010117
language
English
UGent publication?
yes
classification
A2
copyright statement
I have transferred the copyright for this publication to the publisher
id
873294
handle
http://hdl.handle.net/1854/LU-873294
date created
2010-02-22 14:31:52
date last changed
2010-03-29 12:17:17
@article{873294,
  abstract     = {Knowledge about porcine respiratory coronavirus (PRCV) tropism was limited to morphological identification of target cells and controversial reports on replication in macrophages. This study aimed to clarify the lung cell tropism of a Belgian PRCV strain and to examine the lung profile of inflammatory cytokines for 15 days after intratracheal PRCV inoculation of gnotobiotic piglets. Until 5 days after inoculation, more than 50\% of the PRCV-positive cells were type 2 pneumocytes, 30\% bronchiolar epithelial cells, and 10\% macrophages, as demonstrated by immunofluorescence stainings with specific cell markers. In vitro, PRCV productively infected primary porcine lung epithelial cells, but not porcine alveolar macrophages. Bronchoalveolar lavage fluids of PRCV-inoculated pigs contained high levels of interferons (IFN-\ensuremath{\alpha}, IFN-\ensuremath{\gamma}) and interleukin-6, moderate interleukin-12 and low tumour necrosis factor-\ensuremath{\alpha} levels. Our results indicate that PRCV infects primarily type 2 pneumocytes and induces lower lung cytokine levels, compared to swine influenza virus or lipopolysaccharide-complicated PRCV infections.},
  author       = {Atanasova, Kalina and Van Gucht, S and Barb{\'e}, Filip and Lefebvre, David and Chiers, Koen and Van Reeth, Kristien},
  issn         = {1874-3188},
  journal      = {OPEN VETERINARY SCIENCE JOURNAL},
  keyword      = {type 2 pneumocytes,cytokines,PRCV,lungs,alveolar damage},
  language     = {eng},
  pages        = {117--126},
  title        = {Lung cell tropism and inflammatory cytokine-profile of porcine respiratory coronavirus infection},
  url          = {http://dx.doi.org/10.2174/1874318800802010117},
  volume       = {2},
  year         = {2008},
}

Chicago
Atanasova, Kalina, S Van Gucht, Filip Barbé, David Lefebvre, Koen Chiers, and Kristien Van Reeth. 2008. “Lung Cell Tropism and Inflammatory Cytokine-profile of Porcine Respiratory Coronavirus Infection.” Open Veterinary Science Journal 2: 117–126.
APA
Atanasova, K., Van Gucht, S., Barbé, F., Lefebvre, D., Chiers, K., & Van Reeth, K. (2008). Lung cell tropism and inflammatory cytokine-profile of porcine respiratory coronavirus infection. OPEN VETERINARY SCIENCE JOURNAL, 2, 117–126.
Vancouver
1.
Atanasova K, Van Gucht S, Barbé F, Lefebvre D, Chiers K, Van Reeth K. Lung cell tropism and inflammatory cytokine-profile of porcine respiratory coronavirus infection. OPEN VETERINARY SCIENCE JOURNAL. 2008;2:117–26.
MLA
Atanasova, Kalina, S Van Gucht, Filip Barbé, et al. “Lung Cell Tropism and Inflammatory Cytokine-profile of Porcine Respiratory Coronavirus Infection.” OPEN VETERINARY SCIENCE JOURNAL 2 (2008): 117–126. Print.