Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 13.28 MB
Add to list
  1. Search results
  2. Functional analysis of human and feli...

Functional analysis of human and feline coronavirus cross-reactive antibodies directed against the SARS-CoV-2 fusion peptide

Nathalie Vanderheijden (UGent) , Annelies Stevaert, Jiexiong Xie (UGent) , Xiaolei Ren (UGent) , Cyril Barbezange, Sam Noppen, Isabelle Desombere, Bruno Verhasselt (UGent) , Peter Geldhof (UGent) , Nick Vereecke (UGent) , et al.
(2022) FRONTIERS IN IMMUNOLOGY. 12.
Author
Organization
Project
Abstract
To face the continuous emergence of SARS-CoV-2 variants, broadly protective therapeutic antibodies are highly needed. We here focused on the fusion peptide (FP) region of the viral spike antigen since it is highly conserved among alpha- and betacoronaviruses. First, we found that coronavirus cross-reactive antibodies are commonly formed during infection, being omnipresent in sera from COVID-19 patients, in ~50% of pre-pandemic human sera (rich in antibodies against endemic human coronaviruses), and even in feline coronavirus-infected cats. Pepscan analyses demonstrated that a confined N-terminal region of the FP is strongly immunogenic across diverse coronaviruses. Peptide-purified human antibodies targeting this conserved FP epitope exhibited broad binding of alpha- and betacoronaviruses, besides weak and transient SARS-CoV-2 neutralizing activity. Being frequently elicited by coronavirus infection, these FP-binding antibodies might potentially exhibit Fc-mediated effector functions and influence the kinetics or severity of coronavirus infection and disease.
Keywords
Immunology, Immunology and Allergy, SARS-CoV-2, fusion peptide, spike, S2 subunit, broadly neutralizing antibodies, coronaviruses, pepscan

Downloads

  • Download
    Vanderheijden et al. 2021 Functional Analysis of Human and Feline Coronavirus Cross-Reactive Antibodies Directed Against the SARS-CoV-2 Fusion Peptide.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 13.28 MB

Citation

Please use this url to cite or link to this publication:

MLA
Vanderheijden, Nathalie, et al. “Functional Analysis of Human and Feline Coronavirus Cross-Reactive Antibodies Directed against the SARS-CoV-2 Fusion Peptide.” FRONTIERS IN IMMUNOLOGY, vol. 12, 2022, doi:10.3389/fimmu.2021.790415.
APA
Vanderheijden, N., Stevaert, A., Xie, J., Ren, X., Barbezange, C., Noppen, S., … Nauwynck, H. (2022). Functional analysis of human and feline coronavirus cross-reactive antibodies directed against the SARS-CoV-2 fusion peptide. FRONTIERS IN IMMUNOLOGY, 12. https://doi.org/10.3389/fimmu.2021.790415
Chicago author-date
Vanderheijden, Nathalie, Annelies Stevaert, Jiexiong Xie, Xiaolei Ren, Cyril Barbezange, Sam Noppen, Isabelle Desombere, et al. 2022. “Functional Analysis of Human and Feline Coronavirus Cross-Reactive Antibodies Directed against the SARS-CoV-2 Fusion Peptide.” FRONTIERS IN IMMUNOLOGY 12. https://doi.org/10.3389/fimmu.2021.790415.
Chicago author-date (all authors)
Vanderheijden, Nathalie, Annelies Stevaert, Jiexiong Xie, Xiaolei Ren, Cyril Barbezange, Sam Noppen, Isabelle Desombere, Bruno Verhasselt, Peter Geldhof, Nick Vereecke, Veerle Stroobants, Dayoung Oh, Merijn Vanhee, Lieve M. J. Naesens, and Hans Nauwynck. 2022. “Functional Analysis of Human and Feline Coronavirus Cross-Reactive Antibodies Directed against the SARS-CoV-2 Fusion Peptide.” FRONTIERS IN IMMUNOLOGY 12. doi:10.3389/fimmu.2021.790415.
Vancouver
1.
Vanderheijden N, Stevaert A, Xie J, Ren X, Barbezange C, Noppen S, et al. Functional analysis of human and feline coronavirus cross-reactive antibodies directed against the SARS-CoV-2 fusion peptide. FRONTIERS IN IMMUNOLOGY. 2022;12.
IEEE
[1]
N. Vanderheijden et al., “Functional analysis of human and feline coronavirus cross-reactive antibodies directed against the SARS-CoV-2 fusion peptide,” FRONTIERS IN IMMUNOLOGY, vol. 12, 2022.
@article{8732811,
  abstract     = {{To face the continuous emergence of SARS-CoV-2 variants, broadly protective therapeutic antibodies are highly needed. We here focused on the fusion peptide (FP) region of the viral spike antigen since it is highly conserved among alpha- and betacoronaviruses. First, we found that coronavirus cross-reactive antibodies are commonly formed during infection, being omnipresent in sera from COVID-19 patients, in ~50% of pre-pandemic human sera (rich in antibodies against endemic human coronaviruses), and even in feline coronavirus-infected cats. Pepscan analyses demonstrated that a confined N-terminal region of the FP is strongly immunogenic across diverse coronaviruses. Peptide-purified human antibodies targeting this conserved FP epitope exhibited broad binding of alpha- and betacoronaviruses, besides weak and transient SARS-CoV-2 neutralizing activity. Being frequently elicited by coronavirus infection, these FP-binding antibodies might potentially exhibit Fc-mediated effector functions and influence the kinetics or severity of coronavirus infection and disease.}},
  articleno    = {{790415}},
  author       = {{Vanderheijden, Nathalie and Stevaert, Annelies and Xie, Jiexiong and Ren, Xiaolei and Barbezange, Cyril and Noppen, Sam and Desombere, Isabelle and Verhasselt, Bruno and Geldhof, Peter and Vereecke, Nick and Stroobants, Veerle and Oh, Dayoung and Vanhee, Merijn and Naesens, Lieve M. J. and Nauwynck, Hans}},
  issn         = {{1664-3224}},
  journal      = {{FRONTIERS IN IMMUNOLOGY}},
  keywords     = {{Immunology,Immunology and Allergy,SARS-CoV-2,fusion peptide,spike,S2 subunit,broadly neutralizing antibodies,coronaviruses,pepscan}},
  language     = {{eng}},
  pages        = {{17}},
  title        = {{Functional analysis of human and feline coronavirus cross-reactive antibodies directed against the SARS-CoV-2 fusion peptide}},
  url          = {{http://doi.org/10.3389/fimmu.2021.790415}},
  volume       = {{12}},
  year         = {{2022}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: