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Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density

(2022) PLANT AND CELL PHYSIOLOGY. 63(1). p.104-119
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Abstract
The synthetic strigolactone (SL) analog, rac-GR24, has been instrumental in studying the role of SLs as well as karrikins because it activates the receptors DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively. Treatment with rac-GR24 modifies the root architecture at different levels, such as decreasing the lateral root density (LRD), while promoting root hair elongation or flavonol accumulation. Previously, we have shown that the flavonol biosynthesis is transcriptionally activated in the root by rac-GR24 treatment, but, thus far, the molecular players involved in that response have remained unknown. To get an in-depth insight into the changes that occur after the compound is perceived by the roots, we compared the root transcriptomes of the wild type and the more axillary growth2 (max2) mutant, affected in both SL and karrikin signaling pathways, with and without rac-GR24 treatment. Quantitative reverse transcription (qRT)-PCR, reporter line analysis and mutant phenotyping indicated that the flavonol response and the root hair elongation are controlled by the ELONGATED HYPOCOTYL 5 (HY5) and MYB12 transcription factors, but HY5, in contrast to MYB12, affects the LRD as well. Furthermore, we identified the transcription factors TARGET OF MONOPTEROS 5 (TMO5) and TMO5 LIKE1 as negative and the Mediator complex as positive regulators of the rac-GR24 effect on LRD. Altogether, hereby, we get closer toward understanding the molecular mechanisms that underlay the rac-GR24 responses in the root.
Keywords
Cell Biology, Plant Science, Physiology, General Medicine, Flavonols, MAX2, rac-GR24, RNA-seq, Root development, Transcriptional regulation, VASCULAR CELL-DIVISION, SEEDLING DEVELOPMENT, SYSTEM ARCHITECTURE, SHOOT DEVELOPMENT, AUXIN TRANSPORT, GENE-EXPRESSION, PLANT-GROWTH, STRIGOLACTONE, LIGHT, HY5

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Citation

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MLA
Struk, Sylwia Magdalena, et al. “Transcriptional Analysis in the Arabidopsis Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation, Root Hair Elongation and Lateral Root Density.” PLANT AND CELL PHYSIOLOGY, vol. 63, no. 1, 2022, pp. 104–19, doi:10.1093/pcp/pcab149.
APA
Struk, S. M., Braem, L., Matthys, C., Walton, A., Vangheluwe, N., Van Praet, S., … Goormachtig, S. (2022). Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density. PLANT AND CELL PHYSIOLOGY, 63(1), 104–119. https://doi.org/10.1093/pcp/pcab149
Chicago author-date
Struk, Sylwia Magdalena, Lukas Braem, Cedrick Matthys, Alan Walton, Nick Vangheluwe, Stan Van Praet, Lingxiang Jiang, et al. 2022. “Transcriptional Analysis in the Arabidopsis Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation, Root Hair Elongation and Lateral Root Density.” PLANT AND CELL PHYSIOLOGY 63 (1): 104–19. https://doi.org/10.1093/pcp/pcab149.
Chicago author-date (all authors)
Struk, Sylwia Magdalena, Lukas Braem, Cedrick Matthys, Alan Walton, Nick Vangheluwe, Stan Van Praet, Lingxiang Jiang, Pawel Radoslaw Baster, Carolien De Cuyper, François-Didier Boyer, Elisabeth Stes, Tom Beeckman, Jiri Friml, Kris Gevaert, and Sofie Goormachtig. 2022. “Transcriptional Analysis in the Arabidopsis Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation, Root Hair Elongation and Lateral Root Density.” PLANT AND CELL PHYSIOLOGY 63 (1): 104–119. doi:10.1093/pcp/pcab149.
Vancouver
1.
Struk SM, Braem L, Matthys C, Walton A, Vangheluwe N, Van Praet S, et al. Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density. PLANT AND CELL PHYSIOLOGY. 2022;63(1):104–19.
IEEE
[1]
S. M. Struk et al., “Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density,” PLANT AND CELL PHYSIOLOGY, vol. 63, no. 1, pp. 104–119, 2022.
@article{8732047,
  abstract     = {{The synthetic strigolactone (SL) analog, rac-GR24, has been instrumental in studying the role of SLs as well as karrikins because it activates the receptors DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively. Treatment with rac-GR24 modifies the root architecture at different levels, such as decreasing the lateral root density (LRD), while promoting root hair elongation or flavonol accumulation. Previously, we have shown that the flavonol biosynthesis is transcriptionally activated in the root by rac-GR24 treatment, but, thus far, the molecular players involved in that response have remained unknown. To get an in-depth insight into the changes that occur after the compound is perceived by the roots, we compared the root transcriptomes of the wild type and the more axillary growth2 (max2) mutant, affected in both SL and karrikin signaling pathways, with and without rac-GR24 treatment. Quantitative reverse transcription (qRT)-PCR, reporter line analysis and mutant phenotyping indicated that the flavonol response and the root hair elongation are controlled by the ELONGATED HYPOCOTYL 5 (HY5) and MYB12 transcription factors, but HY5, in contrast to MYB12, affects the LRD as well. Furthermore, we identified the transcription factors TARGET OF MONOPTEROS 5 (TMO5) and TMO5 LIKE1 as negative and the Mediator complex as positive regulators of the rac-GR24 effect on LRD. Altogether, hereby, we get closer toward understanding the molecular mechanisms that underlay the rac-GR24 responses in the root.}},
  author       = {{Struk, Sylwia Magdalena and Braem, Lukas and Matthys, Cedrick and Walton, Alan and Vangheluwe, Nick and Van Praet, Stan and Jiang, Lingxiang and Baster, Pawel Radoslaw and De Cuyper, Carolien and Boyer, François-Didier and Stes, Elisabeth and Beeckman, Tom and Friml, Jiri and Gevaert, Kris and Goormachtig, Sofie}},
  issn         = {{0032-0781}},
  journal      = {{PLANT AND CELL PHYSIOLOGY}},
  keywords     = {{Cell Biology,Plant Science,Physiology,General Medicine,Flavonols,MAX2,rac-GR24,RNA-seq,Root development,Transcriptional regulation,VASCULAR CELL-DIVISION,SEEDLING DEVELOPMENT,SYSTEM ARCHITECTURE,SHOOT DEVELOPMENT,AUXIN TRANSPORT,GENE-EXPRESSION,PLANT-GROWTH,STRIGOLACTONE,LIGHT,HY5}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{104--119}},
  title        = {{Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density}},
  url          = {{http://doi.org/10.1093/pcp/pcab149}},
  volume       = {{63}},
  year         = {{2022}},
}

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