Acute hepatopancreatic necrosis disease (AHPND) toxin degradation by Bacillus subtilis DSM33018
- Author
- Ngoc Diem Nguyen (UGent) , Pande Gde Sasmita Julyantoro (UGent) , Md Abul Kashem (UGent) , Sri Kartik Baruah (UGent) and Peter Bossier (UGent)
- Organization
- Abstract
- Early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND), results in significant mortality in penaeid shrimp aquaculture. AHPND is caused by toxins secreted by pathogenic strains of Vibrio parahaemolyticus, that have acquired a unique 63-70 kb AHPND-associated plasmid (pVA1). This plasmid encodes the binary PirA/BVP toxins that consist of two subunits PirAVP and PirBVP. Consequently, the degradation of these toxins might be a valid strategy to control or mitigate AHPND. There is literature-based evidence that the application of Bacillus improves shrimp survival upon challenge with pathogenic V. parahaemolyticus. In this study, Bacillus subtilis DSM33018 strain was shown to degrade AHPND toxins in vitro, as detected by Western blots. Further, an in vivo challenge test, using gnotobiotically cultured brine shrimp Artemia franciscana exposed to PirA/BVP toxins, the DSM33018 Bacillus strain could completely alleviate toxicity. This finding indicates that mitigation of AHPND might include Bacillus-based degradation of PirVP toxins.
- Keywords
- AQUACULTURE, PROBIOTICS, AHPND, Bacillus subtilis, Shrimp, Degradation, PirA, BVP toxin
Downloads
-
(...).pdf
- full text (Published version)
- |
- UGent only
- |
- |
- 3.85 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8731947
- MLA
- Nguyen, Ngoc Diem, et al. “Acute Hepatopancreatic Necrosis Disease (AHPND) Toxin Degradation by Bacillus Subtilis DSM33018.” AQUACULTURE, vol. 540, 2021, doi:10.1016/j.aquaculture.2021.736634.
- APA
- Nguyen, N. D., Julyantoro, P. G. S., Kashem, M. A., Baruah, S. K., & Bossier, P. (2021). Acute hepatopancreatic necrosis disease (AHPND) toxin degradation by Bacillus subtilis DSM33018. AQUACULTURE, 540. https://doi.org/10.1016/j.aquaculture.2021.736634
- Chicago author-date
- Nguyen, Ngoc Diem, Pande Gde Sasmita Julyantoro, Md Abul Kashem, Sri Kartik Baruah, and Peter Bossier. 2021. “Acute Hepatopancreatic Necrosis Disease (AHPND) Toxin Degradation by Bacillus Subtilis DSM33018.” AQUACULTURE 540. https://doi.org/10.1016/j.aquaculture.2021.736634.
- Chicago author-date (all authors)
- Nguyen, Ngoc Diem, Pande Gde Sasmita Julyantoro, Md Abul Kashem, Sri Kartik Baruah, and Peter Bossier. 2021. “Acute Hepatopancreatic Necrosis Disease (AHPND) Toxin Degradation by Bacillus Subtilis DSM33018.” AQUACULTURE 540. doi:10.1016/j.aquaculture.2021.736634.
- Vancouver
- 1.Nguyen ND, Julyantoro PGS, Kashem MA, Baruah SK, Bossier P. Acute hepatopancreatic necrosis disease (AHPND) toxin degradation by Bacillus subtilis DSM33018. AQUACULTURE. 2021;540.
- IEEE
- [1]N. D. Nguyen, P. G. S. Julyantoro, M. A. Kashem, S. K. Baruah, and P. Bossier, “Acute hepatopancreatic necrosis disease (AHPND) toxin degradation by Bacillus subtilis DSM33018,” AQUACULTURE, vol. 540, 2021.
@article{8731947,
abstract = {{Early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND), results in significant mortality in penaeid shrimp aquaculture. AHPND is caused by toxins secreted by pathogenic strains of Vibrio parahaemolyticus, that have acquired a unique 63-70 kb AHPND-associated plasmid (pVA1). This plasmid encodes the binary PirA/BVP toxins that consist of two subunits PirAVP and PirBVP. Consequently, the degradation of these toxins might be a valid strategy to control or mitigate AHPND. There is literature-based evidence that the application of Bacillus improves shrimp survival upon challenge with pathogenic V. parahaemolyticus. In this study, Bacillus subtilis DSM33018 strain was shown to degrade AHPND toxins in vitro, as detected by Western blots. Further, an in vivo challenge test, using gnotobiotically cultured brine shrimp Artemia franciscana exposed to PirA/BVP toxins, the DSM33018 Bacillus strain could completely alleviate toxicity. This finding indicates that mitigation of AHPND might include Bacillus-based degradation of PirVP toxins.}},
articleno = {{736634}},
author = {{Nguyen, Ngoc Diem and Julyantoro, Pande Gde Sasmita and Kashem, Md Abul and Baruah, Sri Kartik and Bossier, Peter}},
issn = {{0044-8486}},
journal = {{AQUACULTURE}},
keywords = {{AQUACULTURE,PROBIOTICS,AHPND,Bacillus subtilis,Shrimp,Degradation,PirA,BVP toxin}},
language = {{eng}},
pages = {{6}},
title = {{Acute hepatopancreatic necrosis disease (AHPND) toxin degradation by Bacillus subtilis DSM33018}},
url = {{http://doi.org/10.1016/j.aquaculture.2021.736634}},
volume = {{540}},
year = {{2021}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: