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Black phosphorus mediated photoporation : a broad absorption nanoplatform for intracellular delivery of macromolecules

Jielin Wang (UGent) , Aranit Harizaj (UGent) , Yongbo Wu, Xiaofang Jiang, Toon Brans (UGent) , Juan Fraire (UGent) , Julián Mejía Morales (UGent) , Stefaan De Smedt (UGent) , Zhilie Tang, Ranhua Xiong (UGent) , et al.
(2021) NANOSCALE. 13(40). p.17049-17056
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Abstract
Nanoparticle-sensitized photoporation for intracellular delivery of external compounds usually relies on the use of spherical gold nanoparticles as sensitizing nanoparticles. As they need stimulation with visible laser light, they are less suited for transfection of cells in thick biological tissues. In this work, we have explored black phosphorus quantum dots (BPQDs) as alternative sensitizing nanoparticles for photoporation with a broad and uniform absorption spectrum from the visible to the near infra-red (NIR) range. We demonstrate that BPQD sensitized photoporation allows efficient intracellular delivery of both siRNA (>80%) and mRNA (>40%) in adherent cells as well as in suspension cells. Cell viability remained high (>80%) irrespective of whether irradiation was performed with visible (532 nm) or near infrared (800 nm) pulsed laser light. Finally, as a proof of concept, we used BPQD sensitized photoporation to deliver macromolecules in cells with thick phantom tissue in the optical path. NIR laser irradiation resulted in only 1.3x reduction in delivery efficiency as compared to photoporation without the phantom gel, while with visible laser light the delivery efficiency was reduced 2x.
Keywords
SMALL INTERFERING RNA, FEMTOSECOND LASER, GENE-GUN, CELLS, GOLD, NANOPARTICLES, TRANSFECTION

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MLA
Wang, Jielin, et al. “Black Phosphorus Mediated Photoporation : A Broad Absorption Nanoplatform for Intracellular Delivery of Macromolecules.” NANOSCALE, vol. 13, no. 40, 2021, pp. 17049–56, doi:10.1039/d1nr05461a.
APA
Wang, J., Harizaj, A., Wu, Y., Jiang, X., Brans, T., Fraire, J., … Braeckmans, K. (2021). Black phosphorus mediated photoporation : a broad absorption nanoplatform for intracellular delivery of macromolecules. NANOSCALE, 13(40), 17049–17056. https://doi.org/10.1039/d1nr05461a
Chicago author-date
Wang, Jielin, Aranit Harizaj, Yongbo Wu, Xiaofang Jiang, Toon Brans, Juan Fraire, Julián Mejía Morales, et al. 2021. “Black Phosphorus Mediated Photoporation : A Broad Absorption Nanoplatform for Intracellular Delivery of Macromolecules.” NANOSCALE 13 (40): 17049–56. https://doi.org/10.1039/d1nr05461a.
Chicago author-date (all authors)
Wang, Jielin, Aranit Harizaj, Yongbo Wu, Xiaofang Jiang, Toon Brans, Juan Fraire, Julián Mejía Morales, Stefaan De Smedt, Zhilie Tang, Ranhua Xiong, and Kevin Braeckmans. 2021. “Black Phosphorus Mediated Photoporation : A Broad Absorption Nanoplatform for Intracellular Delivery of Macromolecules.” NANOSCALE 13 (40): 17049–17056. doi:10.1039/d1nr05461a.
Vancouver
1.
Wang J, Harizaj A, Wu Y, Jiang X, Brans T, Fraire J, et al. Black phosphorus mediated photoporation : a broad absorption nanoplatform for intracellular delivery of macromolecules. NANOSCALE. 2021;13(40):17049–56.
IEEE
[1]
J. Wang et al., “Black phosphorus mediated photoporation : a broad absorption nanoplatform for intracellular delivery of macromolecules,” NANOSCALE, vol. 13, no. 40, pp. 17049–17056, 2021.
@article{8730689,
  abstract     = {{Nanoparticle-sensitized photoporation for intracellular delivery of external compounds usually relies on the use of spherical gold nanoparticles as sensitizing nanoparticles. As they need stimulation with visible laser light, they are less suited for transfection of cells in thick biological tissues. In this work, we have explored black phosphorus quantum dots (BPQDs) as alternative sensitizing nanoparticles for photoporation with a broad and uniform absorption spectrum from the visible to the near infra-red (NIR) range. We demonstrate that BPQD sensitized photoporation allows efficient intracellular delivery of both siRNA (>80%) and mRNA (>40%) in adherent cells as well as in suspension cells. Cell viability remained high (>80%) irrespective of whether irradiation was performed with visible (532 nm) or near infrared (800 nm) pulsed laser light. Finally, as a proof of concept, we used BPQD sensitized photoporation to deliver macromolecules in cells with thick phantom tissue in the optical path. NIR laser irradiation resulted in only 1.3x reduction in delivery efficiency as compared to photoporation without the phantom gel, while with visible laser light the delivery efficiency was reduced 2x.}},
  author       = {{Wang, Jielin and Harizaj, Aranit and Wu, Yongbo and Jiang, Xiaofang and Brans, Toon and Fraire, Juan and Mejía Morales, Julián and De Smedt, Stefaan and Tang, Zhilie and Xiong, Ranhua and Braeckmans, Kevin}},
  issn         = {{2040-3364}},
  journal      = {{NANOSCALE}},
  keywords     = {{SMALL INTERFERING RNA,FEMTOSECOND LASER,GENE-GUN,CELLS,GOLD,NANOPARTICLES,TRANSFECTION}},
  language     = {{eng}},
  number       = {{40}},
  pages        = {{17049--17056}},
  title        = {{Black phosphorus mediated photoporation : a broad absorption nanoplatform for intracellular delivery of macromolecules}},
  url          = {{http://dx.doi.org/10.1039/d1nr05461a}},
  volume       = {{13}},
  year         = {{2021}},
}

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