@inproceedings{8729466,
  abstract     = {{The failure of drug efficacy in clinical trials remains a big issue in cancer research. This is largely due to the limitations of two-dimensional (2D) cell cultures, the most used tool in drug screening. Nowadays, three-dimensional (3D) cultures, including spheroids, are acknowledged to be a better model of the in vivo environment. Immunogenic cell death (ICD) has the potential to be exploited in immunotherapy by activating the host’s own immune system against the cancerous cells, but analysis of ICD induction (including ferroptosis) in 3D cultures has not been performed so far. In this work, we have optimized cell death induction in tumor spheroids. Our cell death analysis method (3DELTA) uses Sytox dyes as a cell death marker and Triton X-100, which efficiently permeabilizes all cells in spheroids, was used to establish 100% cell death. The 3DELTA method was able to detect cell death type without the need to disaggregate spheroids. Moreover, in this work we also demonstrated that 2D experiments cannot be extrapolated to 3D cultures as 3D cultures are less sensitive to cell death induction. Ferroptotic cell death induction in spheroids lead to 50% of cell death whereas in 2D cultures, this caused 90% of cell death. The tumor microenvironment is an important factor that can affect the outcome of anti-cancer therapy. By adding endothelial cells (EC) to cancer cells (CC) in an optimized ratio (EC:CC), we were able to form vascularized spheroids. In conclusion, these spheroids will be a more relevant model for in vivo tumors by analyzing interactions between the different cell types and can be further used for analysis of their immunogenic potential.}},
  articleno    = {{13.21}},
  author       = {{Demuynck, Robin and Efimova, Iuliia and Krysko, Olga and Declercq, Heidi and Krysko, Dmitri}},
  booktitle    = {{JOURNAL OF IMMUNOLOGY}},
  issn         = {{0022-1767}},
  keywords     = {{TME,spheroids,cancer,ferroptosis}},
  language     = {{eng}},
  location     = {{Online}},
  number       = {{Supplement 1}},
  title        = {{Analysis of immunogenic cell death in the tumor microenvironment of tumor spheroids}},
  url          = {{https://www.webofscience.com/wos/woscc/full-record/WOS:000713665800067}},
  volume       = {{206}},
  year         = {{2021}},
}

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