@article{8728596,
  abstract     = {{Background Anti beta 2glycoprotein I (a beta 2GPI) and anticardiolipin (aCL) IgG/IgM show differences in positive/negative agreement and titers between solid phase platforms. Method-specific semiquantitative categorization of titers could improve and harmonize the interpretation across platforms. Aim To evaluate the traditional 40/80-unit thresholds used for aCL and a beta 2GPI for categorization into moderate/high positivity with different analytical systems, and to compare with alternative thresholds. Material and methods aCL and a beta 2GPI thresholds were calculated for two automated systems (chemiluminescent immunoassay [CLIA] and multiplex flow immunoassay [MFI]) by receiver operating characteristic curve analysis on 1108 patient samples, including patients with and without antiphospholipid syndrome (APS), and confirmed on a second population (n = 279). Alternatively, regression analysis on diluted standard material was applied to identify thresholds. Thresholds were compared to 40/80 threshold measured by an enzyme-linked immunosorbent assay (ELISA). Additionally, likelihood ratios (LR) were calculated. Results Threshold levels of 40/80 units show poor agreement between ELISA and automated platforms for classification into low/moderate/high positivity, especially for aCL/a beta 2GPI IgG. Agreement for semiquantitative interpretation of antiphospholipid antibodies (aPL) IgG between ELISA and CLIA/MFI improves with alternative thresholds. LR for aPL IgG increase for thrombotic and obstetric APS based on 40/80 thresholds for ELISA and adapted thresholds for the other systems, but not for IgM. Conclusion Use of 40/80 units as medium/high thresholds is acceptable for aCL/a beta 2GPI IgG ELISA, but not for CLIA and MFI. Alternative semiquantitative thresholds for non-ELISA platforms can be determined by a clinical approach or by using monoclonal antibodies. Semiquantitative reporting of aPL IgM has less impact on increasing probability for APS.}},
  author       = {{Vandevelde, Arne and Chayoua, Walid and de Laat, Bas and Gris, Jean‐Christophe and Moore, Gary W. and Musiał, Jacek and Zuily, Stéphane and Wahl, Denis and Devreese, Katrien}},
  issn         = {{1538-7933}},
  journal      = {{JOURNAL OF THROMBOSIS AND HAEMOSTASIS}},
  keywords     = {{antiphospholipid antibodies,classification,immunoassay,risk,thresholds,ANTIPHOSPHOLIPID ANTIBODIES,CLASSIFICATION CRITERIA,DIAGNOSIS,THROMBOSIS,ASSAY,MULTICENTER,CONSENSUS,GUIDANCE,DEFINE,UPDATE}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{508--524}},
  title        = {{Semiquantitative interpretation of anticardiolipin and antiβ2glycoprotein I antibodies measured with various analytical platforms : communication from the ISTH SSC subcommittee on lupus anticoagulant/antiphospholipid antibodies}},
  url          = {{http://doi.org/10.1111/jth.15585}},
  volume       = {{20}},
  year         = {{2022}},
}

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