Ghent University Academic Bibliography

Advanced

Modeling and quantification of cancer cell invasion through collagen type I matrices

Olivier De Wever UGent, An Hendrix UGent, Astrid De Boeck UGent, Wendy Westbroek, Geert Braems UGent, Shahin Emami, Michèle Sabbah, Christian Gespach and Marc Bracke UGent (2010) INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 54(5). p.887-896
abstract
Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment. Considering the contribution of the stromal environment, we developed a membrane-free single-cell and spheroid based complementary model to study cancer invasion through native collagen type-I matrices. Cell morphology is preserved during the assays allowing real time monitoring of invasion-induced changes in cell structure and F-actin organization. Combining these models with computerized quantification permits the calculation of highly reproducible and operator-independent data. These assays are versatile in the use of fluorescent probes and have a flexible kinetic endpoint. Once the optimal experimental conditions are empirically determined, the collagen type-I invasion assays can be used for preclinical validation of small-molecule inhibitors targeting invasion. Initiation and monitoring of the single-cell and spheroid invasion model can be achieved in 8 h (over 3 days) and in 14 h (over 8 days) respectively.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
collagen, invasion, stroma, 3D matrices, ecosystem, BREAST-CANCER, SIGNALING PATHWAYS, GROWTH-FACTOR, TUMOR-CELLS, N-CADHERIN, TGF-BETA, VITRO, DIFFERENTIATION, GENE, MICROENVIRONMENT
journal title
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
Int. J. Dev. Biol.
volume
54
issue
5
pages
887 - 896
Web of Science type
Article
Web of Science id
000279184400013
ISSN
1696-3547
DOI
10.1387/ijdb.092948ow
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
872838
handle
http://hdl.handle.net/1854/LU-872838
date created
2010-02-22 11:43:20
date last changed
2012-11-07 10:56:13
@article{872838,
  abstract     = {Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment. Considering the contribution of the stromal environment, we developed a membrane-free single-cell and spheroid based complementary model to study cancer invasion through native collagen type-I matrices. Cell morphology is preserved during the assays allowing real time monitoring of invasion-induced changes in cell structure and F-actin organization. Combining these models with computerized quantification permits the calculation of highly reproducible and operator-independent data. These assays are versatile in the use of fluorescent probes and have a flexible kinetic endpoint. Once the optimal experimental conditions are empirically determined, the collagen type-I invasion assays can be used for preclinical validation of small-molecule inhibitors targeting invasion. Initiation and monitoring of the single-cell and spheroid invasion model can be achieved in 8 h (over 3 days) and in 14 h (over 8 days) respectively.},
  author       = {De Wever, Olivier and Hendrix, An and De Boeck, Astrid and Westbroek, Wendy and Braems, Geert and Emami, Shahin and Sabbah, Mich{\`e}le and Gespach, Christian and Bracke, Marc},
  issn         = {1696-3547},
  journal      = {INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY},
  keyword      = {collagen,invasion,stroma,3D matrices,ecosystem,BREAST-CANCER,SIGNALING PATHWAYS,GROWTH-FACTOR,TUMOR-CELLS,N-CADHERIN,TGF-BETA,VITRO,DIFFERENTIATION,GENE,MICROENVIRONMENT},
  language     = {eng},
  number       = {5},
  pages        = {887--896},
  title        = {Modeling and quantification of cancer cell invasion through collagen type I matrices},
  url          = {http://dx.doi.org/10.1387/ijdb.092948ow},
  volume       = {54},
  year         = {2010},
}

Chicago
De Wever, Olivier, An Hendrix, Astrid De Boeck, Wendy Westbroek, Geert Braems, Shahin Emami, Michèle Sabbah, Christian Gespach, and Marc Bracke. 2010. “Modeling and Quantification of Cancer Cell Invasion Through Collagen Type I Matrices.” International Journal of Developmental Biology 54 (5): 887–896.
APA
De Wever, O., Hendrix, A., De Boeck, A., Westbroek, W., Braems, G., Emami, S., Sabbah, M., et al. (2010). Modeling and quantification of cancer cell invasion through collagen type I matrices. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 54(5), 887–896.
Vancouver
1.
De Wever O, Hendrix A, De Boeck A, Westbroek W, Braems G, Emami S, et al. Modeling and quantification of cancer cell invasion through collagen type I matrices. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 2010;54(5):887–96.
MLA
De Wever, Olivier, An Hendrix, Astrid De Boeck, et al. “Modeling and Quantification of Cancer Cell Invasion Through Collagen Type I Matrices.” INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 54.5 (2010): 887–896. Print.