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Modeling and quantification of cancer cell invasion through collagen type I matrices

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Abstract
Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment. Considering the contribution of the stromal environment, we developed a membrane-free single-cell and spheroid based complementary model to study cancer invasion through native collagen type-I matrices. Cell morphology is preserved during the assays allowing real time monitoring of invasion-induced changes in cell structure and F-actin organization. Combining these models with computerized quantification permits the calculation of highly reproducible and operator-independent data. These assays are versatile in the use of fluorescent probes and have a flexible kinetic endpoint. Once the optimal experimental conditions are empirically determined, the collagen type-I invasion assays can be used for preclinical validation of small-molecule inhibitors targeting invasion. Initiation and monitoring of the single-cell and spheroid invasion model can be achieved in 8 h (over 3 days) and in 14 h (over 8 days) respectively.
Keywords
collagen, invasion, stroma, 3D matrices, ecosystem, BREAST-CANCER, SIGNALING PATHWAYS, GROWTH-FACTOR, TUMOR-CELLS, N-CADHERIN, TGF-BETA, VITRO, DIFFERENTIATION, GENE, MICROENVIRONMENT

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MLA
De Wever, Olivier et al. “Modeling and Quantification of Cancer Cell Invasion Through Collagen Type I Matrices.” INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 54.5 (2010): 887–896. Print.
APA
De Wever, O., Hendrix, A., De Boeck, A., Westbroek, W., Braems, G., Emami, S., Sabbah, M., et al. (2010). Modeling and quantification of cancer cell invasion through collagen type I matrices. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 54(5), 887–896.
Chicago author-date
De Wever, Olivier, An Hendrix, Astrid De Boeck, Wendy Westbroek, Geert Braems, Shahin Emami, Michèle Sabbah, Christian Gespach, and Marc Bracke. 2010. “Modeling and Quantification of Cancer Cell Invasion Through Collagen Type I Matrices.” International Journal of Developmental Biology 54 (5): 887–896.
Chicago author-date (all authors)
De Wever, Olivier, An Hendrix, Astrid De Boeck, Wendy Westbroek, Geert Braems, Shahin Emami, Michèle Sabbah, Christian Gespach, and Marc Bracke. 2010. “Modeling and Quantification of Cancer Cell Invasion Through Collagen Type I Matrices.” International Journal of Developmental Biology 54 (5): 887–896.
Vancouver
1.
De Wever O, Hendrix A, De Boeck A, Westbroek W, Braems G, Emami S, et al. Modeling and quantification of cancer cell invasion through collagen type I matrices. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 2010;54(5):887–96.
IEEE
[1]
O. De Wever et al., “Modeling and quantification of cancer cell invasion through collagen type I matrices,” INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, vol. 54, no. 5, pp. 887–896, 2010.
@article{872838,
  abstract     = {Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment. Considering the contribution of the stromal environment, we developed a membrane-free single-cell and spheroid based complementary model to study cancer invasion through native collagen type-I matrices. Cell morphology is preserved during the assays allowing real time monitoring of invasion-induced changes in cell structure and F-actin organization. Combining these models with computerized quantification permits the calculation of highly reproducible and operator-independent data. These assays are versatile in the use of fluorescent probes and have a flexible kinetic endpoint. Once the optimal experimental conditions are empirically determined, the collagen type-I invasion assays can be used for preclinical validation of small-molecule inhibitors targeting invasion. Initiation and monitoring of the single-cell and spheroid invasion model can be achieved in 8 h (over 3 days) and in 14 h (over 8 days) respectively.},
  author       = {De Wever, Olivier and Hendrix, An and De Boeck, Astrid and Westbroek, Wendy and Braems, Geert and Emami, Shahin and Sabbah, Michèle and Gespach, Christian and Bracke, Marc},
  issn         = {1696-3547},
  journal      = {INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY},
  keywords     = {collagen,invasion,stroma,3D matrices,ecosystem,BREAST-CANCER,SIGNALING PATHWAYS,GROWTH-FACTOR,TUMOR-CELLS,N-CADHERIN,TGF-BETA,VITRO,DIFFERENTIATION,GENE,MICROENVIRONMENT},
  language     = {eng},
  number       = {5},
  pages        = {887--896},
  title        = {Modeling and quantification of cancer cell invasion through collagen type I matrices},
  url          = {http://dx.doi.org/10.1387/ijdb.092948ow},
  volume       = {54},
  year         = {2010},
}

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