@inproceedings{8728028,
  abstract     = {{Introduction : 
Equine practitioners are frequently confronted with distal limbs wounds 
characterized by chronic inflammation, delayed re-epithelialization, fibroplasia and an 
excessive blood vessel network of poor quality. This wound tissue in called exuberant 
granulation tissue (EGT). Despite a lot of research, the exact mechanisms of EGT formation 
remain unclear which hinders the development of an effective therapy. Our goal was to 
investigate EGT with the aid of SBF-SEM. This allows a very detailed 3D reconstruction of the 
tissue. 

Materials and Methods : 
Upon resection as part of the therapy, EGT biopts were immediately
fixated in Karnovski (no EC approval needed). After fixation, the samples were washed in 0.1M 
cacodylate buffer and postfixed with 1% osmiumtetroxide. Samples were dehydrated by 
increasing alcohol percentages. Next, they were subjected to graded mixtures of acetone and 
EPON. Finally, the samples were embedded in pure EPON and coated with 3nm platinum. A 
total area of 340µm x 340µm was visualized. In the final run, 1000 sections were made from 
80 nm each. With the aid of the AMIRA software, 3D visualizations were constructed from 
regions of interest. 

Results : 
The electron microscopy images and 3D reconstructions clearly show a distinct blood 
vessel network made of an abundant amount of dysfunctional blood vessels. In this peculiar 
wound tissue a lot of interesting things were observed including endothelial hypertrophy, 
extravasation of immune cells, phagocytizing immune cells and different forms of 
angiogenesis. 

Conclusion : 
This dataset shows very nicely the interlace of blood vessels and immune cells in 
an interesting case of impaired wound healing.}},
  articleno    = {{040}},
  author       = {{Du Cheyne, Charis and Couck, Liesbeth and Kremer, Anneke and Pint, Eva and Martens, Ann and De Spiegelaere, Ward}},
  booktitle    = {{Proceedings of the 33rd Virtual Conference of the European Association of Veterinary Anatomists, 28th-30th July 2021}},
  language     = {{eng}},
  location     = {{Online (Ghent, Belgium)}},
  number       = {{1}},
  pages        = {{1}},
  title        = {{Serial block-face scanning electron microscopy (SBF-SEM) of equine exuberant granulation tissue}},
  url          = {{https://onlinelibrary.wiley.com/doi/10.1111/ahe.12759}},
  volume       = {{51}},
  year         = {{2021}},
}