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Identification of full-length cDNA sequences of hop (Humulus lupulus L.) candidate genes of the prenylflavonid pathway

Lina Maloukh UGent, Jelle De Keukeleire, Jaroslav Matousek, Paul D Matthews, Axel Schwekendiek, Arne Heyerick UGent, Denis De Keukeleire UGent, Erik Van Bockstaele UGent and Isabel Roldàn-Ruiz UGent (2009) Acta Horticulturae. 848. p.81-88
abstract
A noticeable shift of interest for hop (Humulus lupulus L.) is ongoing from flavour-active beer constituents to health-beneficial properties of prenylchalcones, mainly desmethylxanthohumol and xanthohumol. Desmethylxanthohumol serves as a pro-estrogen, since it is readily isomerized to 8-prenylnaringenin, the most potent phytoestrogen currently known, while xanthohumol shows an exceptionally broad spectrum of inhibition mechanisms at all stages of carcinogenesis. However, the low availability of the target compounds in hop glandular trichomes prevents the widespread exploitation of the biological properties of these two compounds. A detailed characterization of genes and regulatory elements in the phenylpropanoid pathway leading to prenylchalcones should contribute to a better understanding of the synthesis of bioactive components in hop. Two hop genes displaying similarities to genes of the prenylpropanoid pathway and one transcription factor were identified by combining a cDNA-AFLP screen of hop cones, cDNA-library screening and Rapid Amplification of cDNA Ends (RACE) approaches. In order to carry out a functional analysis of these genes, gene-transfer experiments are currently being carried out using an Agrobacterium-mediated protocol.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
4-coumarate:CoA ligase, cinnamic acid-4 hydroxylase, library screening, H. lupulus, RACE, ARABIDOPSIS-THALIANA, LIGASE GENES, EXPRESSION, XANTHOHUMOL, 4-COUMARATE, CLONING, DNA
in
Acta Horticulturae
Acta Hortic.
editor
Denis De Keukeleire UGent and Kim E Hummer
volume
848
issue title
Proceedings of the second international humulus symposium
pages
81 - 88
publisher
International Society for Horticultural Science (ISHS)
place of publication
Leuven, Belgium
conference name
2nd International Humulus Symposium
conference location
Ghent, Belgium
conference start
2008-09-01
conference end
2008-09-05
Web of Science type
Proceedings Paper
Web of Science id
000291668300008
ISSN
0567-7572
ISBN
9789066057227
language
English
UGent publication?
yes
classification
P1
id
872715
handle
http://hdl.handle.net/1854/LU-872715
alternative location
http://www.actahort.org/books/848/848_8.htm
date created
2010-02-22 11:17:47
date last changed
2011-07-14 09:08:43
@inproceedings{872715,
  abstract     = {A noticeable shift of interest for hop (Humulus lupulus L.) is ongoing from flavour-active beer constituents to health-beneficial properties of prenylchalcones, mainly desmethylxanthohumol and xanthohumol. Desmethylxanthohumol serves as a pro-estrogen, since it is readily isomerized to 8-prenylnaringenin, the most potent phytoestrogen currently known, while xanthohumol shows an exceptionally broad spectrum of inhibition mechanisms at all stages of carcinogenesis. However, the low availability of the target compounds in hop glandular trichomes prevents the widespread exploitation of the biological properties of these two compounds. A detailed characterization of genes and regulatory elements in the phenylpropanoid pathway leading to prenylchalcones should contribute to a better understanding of the synthesis of bioactive components in hop. Two hop genes displaying similarities to genes of the prenylpropanoid pathway and one transcription factor were identified by combining a cDNA-AFLP screen of hop cones, cDNA-library screening and Rapid Amplification of cDNA Ends (RACE) approaches. In order to carry out a functional analysis of these genes, gene-transfer experiments are currently being carried out using an Agrobacterium-mediated protocol.},
  author       = {Maloukh, Lina and De Keukeleire, Jelle and Matousek, Jaroslav and Matthews, Paul D and Schwekendiek, Axel  and Heyerick, Arne and De Keukeleire, Denis and Van Bockstaele, Erik and Rold{\`a}n-Ruiz, Isabel},
  booktitle    = {Acta Horticulturae},
  editor       = {De Keukeleire, Denis and Hummer, Kim E},
  isbn         = {9789066057227},
  issn         = {0567-7572},
  keyword      = {4-coumarate:CoA ligase,cinnamic acid-4 hydroxylase,library screening,H. lupulus,RACE,ARABIDOPSIS-THALIANA,LIGASE GENES,EXPRESSION,XANTHOHUMOL,4-COUMARATE,CLONING,DNA},
  language     = {eng},
  location     = {Ghent, Belgium},
  pages        = {81--88},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Identification of full-length cDNA sequences of hop (Humulus lupulus L.) candidate genes of the prenylflavonid pathway},
  url          = {http://www.actahort.org/books/848/848\_8.htm},
  volume       = {848},
  year         = {2009},
}

Chicago
Maloukh, Lina, Jelle De Keukeleire, Jaroslav Matousek, Paul D Matthews, Axel Schwekendiek, Arne Heyerick, Denis De Keukeleire, Erik Van Bockstaele, and Isabel Roldàn-Ruiz. 2009. “Identification of Full-length cDNA Sequences of Hop (Humulus Lupulus L.) Candidate Genes of the Prenylflavonid Pathway.” In Acta Horticulturae, ed. Denis De Keukeleire and Kim E Hummer, 848:81–88. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Maloukh, L., De Keukeleire, J., Matousek, J., Matthews, P. D., Schwekendiek, A., Heyerick, A., De Keukeleire, D., et al. (2009). Identification of full-length cDNA sequences of hop (Humulus lupulus L.) candidate genes of the prenylflavonid pathway. In D. De Keukeleire & K. E. Hummer (Eds.), Acta Horticulturae (Vol. 848, pp. 81–88). Presented at the 2nd International Humulus Symposium, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Maloukh L, De Keukeleire J, Matousek J, Matthews PD, Schwekendiek A, Heyerick A, et al. Identification of full-length cDNA sequences of hop (Humulus lupulus L.) candidate genes of the prenylflavonid pathway. In: De Keukeleire D, Hummer KE, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2009. p. 81–8.
MLA
Maloukh, Lina, Jelle De Keukeleire, Jaroslav Matousek, et al. “Identification of Full-length cDNA Sequences of Hop (Humulus Lupulus L.) Candidate Genes of the Prenylflavonid Pathway.” Acta Horticulturae. Ed. Denis De Keukeleire & Kim E Hummer. Vol. 848. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2009. 81–88. Print.