@inproceedings{8725920,
  abstract     = {{LC×LC can provide the required resolving power for a satisfactory analysis of complex samples. However, several practical hurdles must be overcome to achieve robust methods. When transferring the sample from the 1D to the 2D, the volume of the fraction and the inadequate refocusing due to the organic content from the 1D mobile phase on the 2D column will inadvertently lead to a loss in peak capacity and sensitivity. Research has tried to provide solutions for maximal suppression of these negative effects by optimizing the modulation. Relatively unaffected of those solvent transfer issues are combinations using aqueous mobile phases in the 1D. In previous work, a novel LC×LC method was developed, whereby a temperature-responsive stationary phase (TRLC) and a fast RP column (TRLCxRPLC) were combined. TRLC is based on a smart polymer, poly(N-isopropylacrylamide), which depicts increasing hydrophobicity for rising temperatures. This type of stationary phase allows for retention and separation in a purely aqueous mobile phase. Thus, full solute refocusing at the 2D column head is obtained, and the dilution problem experienced with all concentration sensitive detectors in comprehensive 2D-LC offers the prospect to be overcome, by using identical column IDs in both dimensions or to even be inverted, by the so far unknown combination of a broader 1D column paired with a narrower 2D column (2.1×1 mm). Further, the possibilities in terms of method development offered by TRLC in 2D-LC shall be further explored, to significantly reduce overall analysis times while maintaining sensitivity. This can be done by the usage of single-mode gradients (temperature-downward or flow gradients), dual-mode gradients, and the injection of large fraction volumes demonstrated through the separation of representative mixtures of small pharmaceuticals, larger biomolecules, and complex natural product extracts, to map the potential of this novel column combination in 2D-LC-UV and -MS.}},
  author       = {{Wicht, Kristina and Baert, Mathijs and Schipperges, Sonja and von Doehren, Norwin and de Villiers, André and Lynen, Frederic}},
  booktitle    = {{The International Chemical Congress of Pacific Basin Societies, Abstracts}},
  language     = {{eng}},
  location     = {{Hawaii, Honolulu}},
  title        = {{Enhancing sensitivity in LC×LC by hyphenation of temperature-responsive with reversed phase liquid chromatography (TRLC×RPLC)}},
  year         = {{2021}},
}