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MISpheroID : a knowledgebase and transparency tool for minimum information in spheroid identity

(2021) NATURE METHODS. 18(11). p.1294-1303
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Abstract
Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility.
Keywords
Cell Biology, Molecular Biology, Biochemistry, Biotechnology, TUMOR SPHEROIDS, METABOLISM, HALLMARK, CULTURE, CELLS

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MLA
Peirsman, Arne, et al. “MISpheroID : A Knowledgebase and Transparency Tool for Minimum Information in Spheroid Identity.” NATURE METHODS, vol. 18, no. 11, 2021, pp. 1294–303, doi:10.1038/s41592-021-01291-4.
APA
Peirsman, A., Blondeel, E., Ahmed, T., Anckaert, J., Audenaert, D., Boterberg, T., … De Wever, O. (2021). MISpheroID : a knowledgebase and transparency tool for minimum information in spheroid identity. NATURE METHODS, 18(11), 1294–1303. https://doi.org/10.1038/s41592-021-01291-4
Chicago author-date
Peirsman, Arne, Eva Blondeel, Tasdiq Ahmed, Jasper Anckaert, Dominique Audenaert, Tom Boterberg, Krisztina Buzas, et al. 2021. “MISpheroID : A Knowledgebase and Transparency Tool for Minimum Information in Spheroid Identity.” NATURE METHODS 18 (11): 1294–1303. https://doi.org/10.1038/s41592-021-01291-4.
Chicago author-date (all authors)
Peirsman, Arne, Eva Blondeel, Tasdiq Ahmed, Jasper Anckaert, Dominique Audenaert, Tom Boterberg, Krisztina Buzas, Neil Carragher, Gastone Castellani, Flávia Castro, Virginie Dangles-Marie, John Dawson, Pascal De Tullio, Elly De Vlieghere, Sandor Dedeyne, Herman Depypere, Akos Diosdi, Ruslan Dmitriev, Helmut Dolznig, Suzanne Fischer, Christian Gespach, Vera Goossens, Jyrki Heino, An Hendrix, Peter Horvath, Leoni A. Kunz-Schughart, Sebastiaan Maes, Christophe Mangodt, Pieter Mestdagh, Soňa Michlíková, Maria José Oliveira, Francesco Pampaloni, Filippo Piccinini, Cláudio Pinheiro, Jennifer Rahn, Stephen M. Robbins, Elina Siljamäki, Patrick Steigemann, Gwen Sys, Shuichi Takayama, Anna Tesei, Joeri Tulkens, Michiel Van Waeyenberge, Jo Vandesompele, Glenn Wagemans, Claudia Weindorfer, Nurten Yigit, Nina Zablowsky, Michele Zanoni, Phillip Blondeel, and Olivier De Wever. 2021. “MISpheroID : A Knowledgebase and Transparency Tool for Minimum Information in Spheroid Identity.” NATURE METHODS 18 (11): 1294–1303. doi:10.1038/s41592-021-01291-4.
Vancouver
1.
Peirsman A, Blondeel E, Ahmed T, Anckaert J, Audenaert D, Boterberg T, et al. MISpheroID : a knowledgebase and transparency tool for minimum information in spheroid identity. NATURE METHODS. 2021;18(11):1294–303.
IEEE
[1]
A. Peirsman et al., “MISpheroID : a knowledgebase and transparency tool for minimum information in spheroid identity,” NATURE METHODS, vol. 18, no. 11, pp. 1294–1303, 2021.
@article{8725528,
  abstract     = {{Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility.}},
  author       = {{Peirsman, Arne and Blondeel, Eva and Ahmed, Tasdiq and Anckaert, Jasper and Audenaert, Dominique and Boterberg, Tom and Buzas, Krisztina and Carragher, Neil and Castellani, Gastone and Castro, Flávia and Dangles-Marie, Virginie and Dawson, John and De Tullio, Pascal and De Vlieghere, Elly and Dedeyne, Sandor and Depypere, Herman and Diosdi, Akos and Dmitriev, Ruslan and Dolznig, Helmut and Fischer, Suzanne and Gespach, Christian and Goossens, Vera and Heino, Jyrki and Hendrix, An and Horvath, Peter and Kunz-Schughart, Leoni A. and Maes, Sebastiaan and Mangodt, Christophe and Mestdagh, Pieter and Michlíková, Soňa and Oliveira, Maria José and Pampaloni, Francesco and Piccinini, Filippo and Pinheiro, Cláudio and Rahn, Jennifer and Robbins, Stephen M. and Siljamäki, Elina and Steigemann, Patrick and Sys, Gwen and Takayama, Shuichi and Tesei, Anna and Tulkens, Joeri and Van Waeyenberge, Michiel and Vandesompele, Jo and Wagemans, Glenn and Weindorfer, Claudia and Yigit, Nurten and Zablowsky, Nina and Zanoni, Michele and Blondeel, Phillip and De Wever, Olivier}},
  issn         = {{1548-7091}},
  journal      = {{NATURE METHODS}},
  keywords     = {{Cell Biology,Molecular Biology,Biochemistry,Biotechnology,TUMOR SPHEROIDS,METABOLISM,HALLMARK,CULTURE,CELLS}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{1294--1303}},
  title        = {{MISpheroID : a knowledgebase and transparency tool for minimum information in spheroid identity}},
  url          = {{http://dx.doi.org/10.1038/s41592-021-01291-4}},
  volume       = {{18}},
  year         = {{2021}},
}

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