
In vitro evaluation of dietary fiber anti-infectious properties against food-borne enterotoxigenic Escherichia coli
- Author
- Thomas Sauvaitre, Claude Durif, Adeline Sivignon, Sandrine Chalancon, Tom Van de Wiele (UGent) , Lucie Etienne-Mesmin and Stephanie Blanquet-Diot
- Organization
- Abstract
- Dietary fibers have well-known beneficial effects on human health, but their anti-infectious properties against human enteric pathogens have been poorly investigated. Enterotoxigenic Escherichia coli (ETEC) is the main agent of travelers' diarrhea, against which targeted preventive strategies are currently lacking. ETEC pathogenesis relies on multiple virulence factors allowing interactions with the intestinal mucosal layer and toxins triggering the onset of diarrheal symptoms. Here, we used complementary in vitro assays to study the antagonistic properties of eight fiber-containing products from cereals, legumes or microbes against the prototypical human ETEC strain H10407. Inhibitory effects of these products on the pathogen were tested through growth, toxin production and mucus/cell adhesion inhibition assays. None of the tested compounds inhibited ETEC strain H10407 growth, while lentil extract was able to decrease heat labile toxin (LT) concentration in culture media. Lentil extract and specific yeast cell walls also interfered with ETEC strain H10407 adhesion to mucin beads and human intestinal cells. These results constitute a first step in the use of dietary fibers as a nutritional strategy to prevent ETEC infection. Further work will be dedicated to the study of fiber/ETEC interactions within a complex gut microbial background.
- Keywords
- dietary fibers, food-borne pathogen, Enterotoxigenic Escherichia coli, virulence, adhesion, intestinal cells, LT toxin, mucus, NATURAL FEED INGREDIENTS, HEAT-LABILE ENTEROTOXIN, GUT MICROBIOTA, ADHESION, COLONIZATION, CHITOSAN, DIARRHEA, SUBUNIT, BINDING, ABILITY
Downloads
-
Sauvaitre-2021-In Vitro Evaluation of Dietary.pdf
- full text (Published version)
- |
- open access
- |
- |
- 1.60 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8724503
- MLA
- Sauvaitre, Thomas, et al. “In Vitro Evaluation of Dietary Fiber Anti-Infectious Properties against Food-Borne Enterotoxigenic Escherichia Coli.” NUTRIENTS, vol. 13, no. 9, 2021, doi:10.3390/nu13093188.
- APA
- Sauvaitre, T., Durif, C., Sivignon, A., Chalancon, S., Van de Wiele, T., Etienne-Mesmin, L., & Blanquet-Diot, S. (2021). In vitro evaluation of dietary fiber anti-infectious properties against food-borne enterotoxigenic Escherichia coli. NUTRIENTS, 13(9). https://doi.org/10.3390/nu13093188
- Chicago author-date
- Sauvaitre, Thomas, Claude Durif, Adeline Sivignon, Sandrine Chalancon, Tom Van de Wiele, Lucie Etienne-Mesmin, and Stephanie Blanquet-Diot. 2021. “In Vitro Evaluation of Dietary Fiber Anti-Infectious Properties against Food-Borne Enterotoxigenic Escherichia Coli.” NUTRIENTS 13 (9). https://doi.org/10.3390/nu13093188.
- Chicago author-date (all authors)
- Sauvaitre, Thomas, Claude Durif, Adeline Sivignon, Sandrine Chalancon, Tom Van de Wiele, Lucie Etienne-Mesmin, and Stephanie Blanquet-Diot. 2021. “In Vitro Evaluation of Dietary Fiber Anti-Infectious Properties against Food-Borne Enterotoxigenic Escherichia Coli.” NUTRIENTS 13 (9). doi:10.3390/nu13093188.
- Vancouver
- 1.Sauvaitre T, Durif C, Sivignon A, Chalancon S, Van de Wiele T, Etienne-Mesmin L, et al. In vitro evaluation of dietary fiber anti-infectious properties against food-borne enterotoxigenic Escherichia coli. NUTRIENTS. 2021;13(9).
- IEEE
- [1]T. Sauvaitre et al., “In vitro evaluation of dietary fiber anti-infectious properties against food-borne enterotoxigenic Escherichia coli,” NUTRIENTS, vol. 13, no. 9, 2021.
@article{8724503, abstract = {{Dietary fibers have well-known beneficial effects on human health, but their anti-infectious properties against human enteric pathogens have been poorly investigated. Enterotoxigenic Escherichia coli (ETEC) is the main agent of travelers' diarrhea, against which targeted preventive strategies are currently lacking. ETEC pathogenesis relies on multiple virulence factors allowing interactions with the intestinal mucosal layer and toxins triggering the onset of diarrheal symptoms. Here, we used complementary in vitro assays to study the antagonistic properties of eight fiber-containing products from cereals, legumes or microbes against the prototypical human ETEC strain H10407. Inhibitory effects of these products on the pathogen were tested through growth, toxin production and mucus/cell adhesion inhibition assays. None of the tested compounds inhibited ETEC strain H10407 growth, while lentil extract was able to decrease heat labile toxin (LT) concentration in culture media. Lentil extract and specific yeast cell walls also interfered with ETEC strain H10407 adhesion to mucin beads and human intestinal cells. These results constitute a first step in the use of dietary fibers as a nutritional strategy to prevent ETEC infection. Further work will be dedicated to the study of fiber/ETEC interactions within a complex gut microbial background.}}, articleno = {{3188}}, author = {{Sauvaitre, Thomas and Durif, Claude and Sivignon, Adeline and Chalancon, Sandrine and Van de Wiele, Tom and Etienne-Mesmin, Lucie and Blanquet-Diot, Stephanie}}, issn = {{2072-6643}}, journal = {{NUTRIENTS}}, keywords = {{dietary fibers,food-borne pathogen,Enterotoxigenic Escherichia coli,virulence,adhesion,intestinal cells,LT toxin,mucus,NATURAL FEED INGREDIENTS,HEAT-LABILE ENTEROTOXIN,GUT MICROBIOTA,ADHESION,COLONIZATION,CHITOSAN,DIARRHEA,SUBUNIT,BINDING,ABILITY}}, language = {{eng}}, number = {{9}}, pages = {{12}}, title = {{In vitro evaluation of dietary fiber anti-infectious properties against food-borne enterotoxigenic Escherichia coli}}, url = {{http://doi.org/10.3390/nu13093188}}, volume = {{13}}, year = {{2021}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: