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Evaluation of Bone Regeneration with an Injectable, In Situ Polymerizable Pluronic (R) F127 Hydrogel Derivative Combined with Autologous Mesenchymal Stem Cells in a Goat Tibia Defect Model

Evi Lippens UGent, Geert Vertenten UGent, Jordi Gironès Molera UGent, Heidi Declercq UGent, Jimmy Saunders UGent, J Luyten, Luc Duchateau UGent, Etienne Schacht UGent, Lieven Vlaminck UGent and Frank Gasthuys UGent, et al. (2010) TISSUE ENGINEERING PART A. 16(2). p.617-627
abstract
In situ forming bone substitute materials are attractive candidates for filling irregularly shaped defects. In this study, a chemically modified form of the Pluronic (R) F127 hydrogel was used. Similar to the parent form, this derivative underwent a sol-gel transition in the body and additional radical curing resulted in a stable three-dimensional network gel with a controllable degradation rate. An extra cell source of autologous bone marrow-derived mesenchymal stem cells was mixed with the hydrogel to increase the ossification process, when implanted in noncritical size unicortical tibia defects. These cells were cultured and predifferentiated on two types of cell carrier systems, that is, gelatin CultiSpher-S (R) microcarriers and hydroxyapatite tubular carriers. Radiographic and histological evaluation revealed that bone regeneration was comparable in the defects with the bone substitute compositions and the untreated control defects at 2 and 4 weeks postimplantation and that newly formed bone originated from the cells on the CultiSpher-S carriers. This resulted, 6 and 8 weeks postimplantation, in faster bone repair in the defects filled with the hydrogel plus CultiSpher-S carriers in comparison to the control defects. Surprisingly, there was no formation of new bone originating from the hydroxyapatite carriers. The hydrogel by itself seemed to stimulate the natural repair process.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CULTURE, RATS, HYDROXYAPATITE, SCAFFOLD, ANIMAL-MODELS, VIVO, MACROPOROUS GELATIN SPHERES, ALLOGRAFT, GRAFT SUBSTITUTES
journal title
TISSUE ENGINEERING PART A
Tissue Eng. Part A
volume
16
issue
2
pages
11 pages
publisher
MARY ANN LIEBERT INC
place of publication
NEW ROCHELLE
Web of Science type
Article
Web of Science id
000274329000026
ISSN
1937-3341
DOI
10.1089/ten.tea.2009.0418
language
English
UGent publication?
yes
classification
A1
id
872348
handle
http://hdl.handle.net/1854/LU-872348
date created
2010-02-22 08:50:37
date last changed
2010-02-25 13:31:46
@article{872348,
  abstract     = {In situ forming bone substitute materials are attractive candidates for filling irregularly shaped defects. In this study, a chemically modified form of the Pluronic (R) F127 hydrogel was used. Similar to the parent form, this derivative underwent a sol-gel transition in the body and additional radical curing resulted in a stable three-dimensional network gel with a controllable degradation rate. An extra cell source of autologous bone marrow-derived mesenchymal stem cells was mixed with the hydrogel to increase the ossification process, when implanted in noncritical size unicortical tibia defects. These cells were cultured and predifferentiated on two types of cell carrier systems, that is, gelatin CultiSpher-S (R) microcarriers and hydroxyapatite tubular carriers. Radiographic and histological evaluation revealed that bone regeneration was comparable in the defects with the bone substitute compositions and the untreated control defects at 2 and 4 weeks postimplantation and that newly formed bone originated from the cells on the CultiSpher-S carriers. This resulted, 6 and 8 weeks postimplantation, in faster bone repair in the defects filled with the hydrogel plus CultiSpher-S carriers in comparison to the control defects. Surprisingly, there was no formation of new bone originating from the hydroxyapatite carriers. The hydrogel by itself seemed to stimulate the natural repair process.},
  author       = {Lippens, Evi and Vertenten, Geert and Giron{\`e}s Molera, Jordi and Declercq, Heidi and Saunders, Jimmy and Luyten, J and Duchateau, Luc and Schacht, Etienne and Vlaminck, Lieven and Gasthuys, Frank and Cornelissen, Maria},
  issn         = {1937-3341},
  journal      = {TISSUE ENGINEERING PART A},
  keyword      = {CULTURE,RATS,HYDROXYAPATITE,SCAFFOLD,ANIMAL-MODELS,VIVO,MACROPOROUS GELATIN SPHERES,ALLOGRAFT,GRAFT SUBSTITUTES},
  language     = {eng},
  number       = {2},
  pages        = {617--627},
  publisher    = {MARY ANN LIEBERT INC},
  title        = {Evaluation of Bone Regeneration with an Injectable, In Situ Polymerizable Pluronic (R) F127 Hydrogel Derivative Combined with Autologous Mesenchymal Stem Cells in a Goat Tibia Defect Model},
  url          = {http://dx.doi.org/10.1089/ten.tea.2009.0418},
  volume       = {16},
  year         = {2010},
}

Chicago
Lippens, Evi, Geert Vertenten, Jordi Gironès Molera, Heidi Declercq, Jimmy Saunders, J Luyten, Luc Duchateau, et al. 2010. “Evaluation of Bone Regeneration with an Injectable, In Situ Polymerizable Pluronic (R) F127 Hydrogel Derivative Combined with Autologous Mesenchymal Stem Cells in a Goat Tibia Defect Model.” Tissue Engineering Part A 16 (2): 617–627.
APA
Lippens, E., Vertenten, G., Gironès Molera, J., Declercq, H., Saunders, J., Luyten, J., Duchateau, L., et al. (2010). Evaluation of Bone Regeneration with an Injectable, In Situ Polymerizable Pluronic (R) F127 Hydrogel Derivative Combined with Autologous Mesenchymal Stem Cells in a Goat Tibia Defect Model. TISSUE ENGINEERING PART A, 16(2), 617–627.
Vancouver
1.
Lippens E, Vertenten G, Gironès Molera J, Declercq H, Saunders J, Luyten J, et al. Evaluation of Bone Regeneration with an Injectable, In Situ Polymerizable Pluronic (R) F127 Hydrogel Derivative Combined with Autologous Mesenchymal Stem Cells in a Goat Tibia Defect Model. TISSUE ENGINEERING PART A. NEW ROCHELLE: MARY ANN LIEBERT INC; 2010;16(2):617–27.
MLA
Lippens, Evi, Geert Vertenten, Jordi Gironès Molera, et al. “Evaluation of Bone Regeneration with an Injectable, In Situ Polymerizable Pluronic (R) F127 Hydrogel Derivative Combined with Autologous Mesenchymal Stem Cells in a Goat Tibia Defect Model.” TISSUE ENGINEERING PART A 16.2 (2010): 617–627. Print.