- Author
- Bing Liu, Chunlian Jin, Nico De Storme (UGent) , Sébastien Schotte, Cédric Schindfessel (UGent) , Tim De Meyer (UGent) and Danny Geelen (UGent)
- Organization
- Abstract
- Meiosis drives reciprocal genetic exchanges and produces gametes with halved chromosome number, which is important for the genetic diversity, plant viability, and ploidy consistency of flowering plants. Alterations in chromosome dynamics and/or cytokinesis during meiosis may lead to meiotic restitution and the formation of unreduced microspores. In this study, we isolated an Arabidopsis mutant male meiotic restitution 1 (mmr1), which produces a small subpopulation of diploid or polyploid pollen grains. Cytological analysis revealed that mmr1 produces dyads, triads, and monads indicative of male meiotic restitution. Both homologous chromosomes and sister chromatids in mmr1 are separated normally, but chromosome condensation at metaphase I is slightly affected. The mmr1 mutant displayed incomplete meiotic cytokinesis. Supportively, immunostaining of the microtubular cytoskeleton showed that the spindle organization at anaphase II and mini-phragmoplast formation at telophase II are aberrant. The causative mutation in mmr1 was mapped to chromosome 1 at the chromatin regulator Male Meiocyte Death 1 (MMD1/DUET) locus. mmr1 contains a C-to-T transition at the third exon of MMD1/DUET at the genomic position 2168 bp from the start codon, which causes an amino acid change G618D that locates in the conserved PHD-finger domain of histone binding proteins. The F1 progenies of mmr1 crossing with knockout mmd1/duet mutant exhibited same meiotic defects and similar meiotic restitution rate as mmr1. Taken together, we here report a hypomorphic mmd1/duet allele that typically shows defects in microtubule organization and cytokinesis.
- Keywords
- meiosis, meiotic restitution, meiotic cytokinesis, unreduced gametes, MMD1, UNREDUCED GAMETE FORMATION, WHOLE GENOME DUPLICATIONS, MALE MEIOTIC CYTOKINESIS, ARABIDOPSIS-THALIANA, CENTROMERE COHESION, FINGER PROTEIN, MALE-FERTILITY, GENE, MECHANISMS, MEIOSIS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8721931
- MLA
- Liu, Bing, et al. “A Hypomorphic Mutant of PHD Domain Protein Male Meiocytes Death 1.” GENES, vol. 12, no. 4, 2021, doi:10.3390/genes12040516.
- APA
- Liu, B., Jin, C., De Storme, N., Schotte, S., Schindfessel, C., De Meyer, T., & Geelen, D. (2021). A hypomorphic mutant of PHD domain protein male meiocytes death 1. GENES, 12(4). https://doi.org/10.3390/genes12040516
- Chicago author-date
- Liu, Bing, Chunlian Jin, Nico De Storme, Sébastien Schotte, Cédric Schindfessel, Tim De Meyer, and Danny Geelen. 2021. “A Hypomorphic Mutant of PHD Domain Protein Male Meiocytes Death 1.” GENES 12 (4). https://doi.org/10.3390/genes12040516.
- Chicago author-date (all authors)
- Liu, Bing, Chunlian Jin, Nico De Storme, Sébastien Schotte, Cédric Schindfessel, Tim De Meyer, and Danny Geelen. 2021. “A Hypomorphic Mutant of PHD Domain Protein Male Meiocytes Death 1.” GENES 12 (4). doi:10.3390/genes12040516.
- Vancouver
- 1.Liu B, Jin C, De Storme N, Schotte S, Schindfessel C, De Meyer T, et al. A hypomorphic mutant of PHD domain protein male meiocytes death 1. GENES. 2021;12(4).
- IEEE
- [1]B. Liu et al., “A hypomorphic mutant of PHD domain protein male meiocytes death 1,” GENES, vol. 12, no. 4, 2021.
@article{8721931,
abstract = {{Meiosis drives reciprocal genetic exchanges and produces gametes with halved chromosome number, which is important for the genetic diversity, plant viability, and ploidy consistency of flowering plants. Alterations in chromosome dynamics and/or cytokinesis during meiosis may lead to meiotic restitution and the formation of unreduced microspores. In this study, we isolated an Arabidopsis mutant male meiotic restitution 1 (mmr1), which produces a small subpopulation of diploid or polyploid pollen grains. Cytological analysis revealed that mmr1 produces dyads, triads, and monads indicative of male meiotic restitution. Both homologous chromosomes and sister chromatids in mmr1 are separated normally, but chromosome condensation at metaphase I is slightly affected. The mmr1 mutant displayed incomplete meiotic cytokinesis. Supportively, immunostaining of the microtubular cytoskeleton showed that the spindle organization at anaphase II and mini-phragmoplast formation at telophase II are aberrant. The causative mutation in mmr1 was mapped to chromosome 1 at the chromatin regulator Male Meiocyte Death 1 (MMD1/DUET) locus. mmr1 contains a C-to-T transition at the third exon of MMD1/DUET at the genomic position 2168 bp from the start codon, which causes an amino acid change G618D that locates in the conserved PHD-finger domain of histone binding proteins. The F1 progenies of mmr1 crossing with knockout mmd1/duet mutant exhibited same meiotic defects and similar meiotic restitution rate as mmr1. Taken together, we here report a hypomorphic mmd1/duet allele that typically shows defects in microtubule organization and cytokinesis.}},
articleno = {{516}},
author = {{Liu, Bing and Jin, Chunlian and De Storme, Nico and Schotte, Sébastien and Schindfessel, Cédric and De Meyer, Tim and Geelen, Danny}},
issn = {{2073-4425}},
journal = {{GENES}},
keywords = {{meiosis,meiotic restitution,meiotic cytokinesis,unreduced gametes,MMD1,UNREDUCED GAMETE FORMATION,WHOLE GENOME DUPLICATIONS,MALE MEIOTIC CYTOKINESIS,ARABIDOPSIS-THALIANA,CENTROMERE COHESION,FINGER PROTEIN,MALE-FERTILITY,GENE,MECHANISMS,MEIOSIS}},
language = {{eng}},
number = {{4}},
pages = {{14}},
title = {{A hypomorphic mutant of PHD domain protein male meiocytes death 1}},
url = {{http://doi.org/10.3390/genes12040516}},
volume = {{12}},
year = {{2021}},
}
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