- Author
- Leentje De Puysseleyr (UGent) , Kristien De Puysseleyr (UGent) , Joanna Rybarczyk (UGent) , Paulien Vander Donck (UGent) , Winnok H. De Vos and Daisy Vanrompay (UGent)
- Organization
- Abstract
- Chlamydia suis (C. suis) resides in the intestines of pigs and tetracycline-resistant strains are emerging worldwide. Intestinal infections are often subclinical. However, the gut is regarded as a C. suis reservoir and clinical infections have been associated with enteritis, conjunctivitis, pneumonia and reproductive failure. C. suis was found in boar semen and venereal transmission occurred. We studied the anti-Chlamydia suis activity of ovotransferrin (ovoTF) and bovine lactoferrin (bLF). Pre-incubation of C. suis with bLF or ovoTF had no significant effect on overall chlamydia replication (mean fluorescence area) in McCoy cells. The addition of ovoTF to the culture medium had no effect on bacterial replication, but the addition of 0.5 or 5 mg/mL of bLF significantly reduced the inclusion size by 17% and 15% respectively. Egg components are used for cryopreservation of boar semen. When inoculating an ovoTF-containing and Chlamydia suis-spiked semen sample in McCoy cells, a significant reduction in inclusion number (by 7%) and overall replication (by 11%) was observed. Thus, we showed that transferrins possess anti-chlamydial activity. Moreover, ovoTF addition to semen extenders might reduce C. suis venereal transmission. Further research is needed to unravel the mechanisms behind the observations and to enhance the effect of transferrins on C. suis.
- Keywords
- BOVINE LACTOFERRIN, OUTER-MEMBRANE, OVOTRANSFERRIN, BACTERIAL, PSITTACI, DAMAGE, ANTIBACTERIAL, SELECTION, RELEASE, GROWTH, Chlamydia suis, swine, transferrin, ovotransferrin, lactoferrin
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8721867
- MLA
- De Puysseleyr, Leentje, et al. “Transferrins Reduce Replication of Chlamydia Suis in McCoy Cells.” PATHOGENS, vol. 10, no. 7, 2021, doi:10.3390/pathogens10070858.
- APA
- De Puysseleyr, L., De Puysseleyr, K., Rybarczyk, J., Vander Donck, P., De Vos, W. H., & Vanrompay, D. (2021). Transferrins reduce replication of Chlamydia suis in McCoy cells. PATHOGENS, 10(7). https://doi.org/10.3390/pathogens10070858
- Chicago author-date
- De Puysseleyr, Leentje, Kristien De Puysseleyr, Joanna Rybarczyk, Paulien Vander Donck, Winnok H. De Vos, and Daisy Vanrompay. 2021. “Transferrins Reduce Replication of Chlamydia Suis in McCoy Cells.” PATHOGENS 10 (7). https://doi.org/10.3390/pathogens10070858.
- Chicago author-date (all authors)
- De Puysseleyr, Leentje, Kristien De Puysseleyr, Joanna Rybarczyk, Paulien Vander Donck, Winnok H. De Vos, and Daisy Vanrompay. 2021. “Transferrins Reduce Replication of Chlamydia Suis in McCoy Cells.” PATHOGENS 10 (7). doi:10.3390/pathogens10070858.
- Vancouver
- 1.De Puysseleyr L, De Puysseleyr K, Rybarczyk J, Vander Donck P, De Vos WH, Vanrompay D. Transferrins reduce replication of Chlamydia suis in McCoy cells. PATHOGENS. 2021;10(7).
- IEEE
- [1]L. De Puysseleyr, K. De Puysseleyr, J. Rybarczyk, P. Vander Donck, W. H. De Vos, and D. Vanrompay, “Transferrins reduce replication of Chlamydia suis in McCoy cells,” PATHOGENS, vol. 10, no. 7, 2021.
@article{8721867, abstract = {{Chlamydia suis (C. suis) resides in the intestines of pigs and tetracycline-resistant strains are emerging worldwide. Intestinal infections are often subclinical. However, the gut is regarded as a C. suis reservoir and clinical infections have been associated with enteritis, conjunctivitis, pneumonia and reproductive failure. C. suis was found in boar semen and venereal transmission occurred. We studied the anti-Chlamydia suis activity of ovotransferrin (ovoTF) and bovine lactoferrin (bLF). Pre-incubation of C. suis with bLF or ovoTF had no significant effect on overall chlamydia replication (mean fluorescence area) in McCoy cells. The addition of ovoTF to the culture medium had no effect on bacterial replication, but the addition of 0.5 or 5 mg/mL of bLF significantly reduced the inclusion size by 17% and 15% respectively. Egg components are used for cryopreservation of boar semen. When inoculating an ovoTF-containing and Chlamydia suis-spiked semen sample in McCoy cells, a significant reduction in inclusion number (by 7%) and overall replication (by 11%) was observed. Thus, we showed that transferrins possess anti-chlamydial activity. Moreover, ovoTF addition to semen extenders might reduce C. suis venereal transmission. Further research is needed to unravel the mechanisms behind the observations and to enhance the effect of transferrins on C. suis.}}, articleno = {{858}}, author = {{De Puysseleyr, Leentje and De Puysseleyr, Kristien and Rybarczyk, Joanna and Vander Donck, Paulien and De Vos, Winnok H. and Vanrompay, Daisy}}, issn = {{2076-0817}}, journal = {{PATHOGENS}}, keywords = {{BOVINE LACTOFERRIN,OUTER-MEMBRANE,OVOTRANSFERRIN,BACTERIAL,PSITTACI,DAMAGE,ANTIBACTERIAL,SELECTION,RELEASE,GROWTH,Chlamydia suis,swine,transferrin,ovotransferrin,lactoferrin}}, language = {{eng}}, number = {{7}}, pages = {{10}}, title = {{Transferrins reduce replication of Chlamydia suis in McCoy cells}}, url = {{http://doi.org/10.3390/pathogens10070858}}, volume = {{10}}, year = {{2021}}, }
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