@article{8721484,
  abstract     = {{The antibacterial and cell-proliferative character of atmospheric pressure plasma jets (APPJs) helps in the healing process of chronic wounds. However, control of the plasma-biological target interface remains an open issue. High vacuum ultraviolet/ultraviolet (VUV/UV) radiation and RONS flux from plasma may cause damage of a treated tissue; therefore, controlled interaction is essential. VUV/UV emission from argon APPJs and radiation control with aerosol injection in plasma effluent is the focus of this research. The aerosol effect on radiation is studied by a fluorescent target capable of resolving the plasma oxidation footprint. In addition, DNA damage is evaluated by plasmid DNA radiation assay and cell proliferation assay to assess safety aspects of the plasma jet, the effect of VUV/UV radiation, and its control with aerosol injection. Inevitable emission of VUV/UV radiation from plasmas during treatment is demonstrated in this work. Plasma has no antiproliferative effect on fibroblasts in short treatments (t < 60 s), while long exposure has a cytotoxic effect, resulting in decreased cell survival. Radiation has no effect on cell survival in the medium due to absorption. However, a strong cytotoxic effect on the attached fibroblasts without the medium is apparent. VUV/UV radiation contributes 70% of the integral plasma effect in induction of single- and double-strand DNA breaks and cytotoxicity of the attached cells without the medium. Survival of the attached cells increases by 10% when aerosol is introduced between plasma and the cells. Injection of aerosol in the plasma effluent can help to control the plasma-cell/tissue interaction. Aerosol droplets in the effluent partially absorb UV emission from the plasma, limiting photon flux in the direction of the biological target. Herein, cold and safe plasma-aerosol treatment and a safe operational mode of treatment are demonstrated in a murine model.}},
  author       = {{Sremački, Ivana and Kos, Spela and Bosnjak, Masa and Jurov, Andrea and Sersa, Gregor and Modic, Martina and Leys, Christophe and Cvelbar, Uros and Nikiforov, Anton}},
  issn         = {{1944-8244}},
  journal      = {{ACS APPLIED MATERIALS & INTERFACES}},
  keywords     = {{plasma-aerosol,UV radiation from plasma,interface control,plasma damage control,biomedical application,DNA-DAMAGE,JET,INACTIVATION,RADIATION,BACTERIA,2',7'-DICHLORODIHYDROFLUORESCEIN,DECONTAMINATION,IDENTIFICATION}},
  language     = {{eng}},
  number       = {{39}},
  pages        = {{46303--46316}},
  title        = {{Plasma damage control : from biomolecules to cells and skin}},
  url          = {{http://doi.org/10.1021/acsami.1c12232}},
  volume       = {{13}},
  year         = {{2021}},
}

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