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The long non-coding RNA SAMMSON is essential for uveal melanoma cell survival

Shanna Dewaele (UGent) , Louis Delhaye (UGent) , Boel De Paepe (UGent) , Eric de Bony de Lavergne (UGent) , Jilke De Wilde (UGent) , Katrien Vanderheyden (UGent) , Jasper Anckaert (UGent) , Nurten Yigit (UGent) , Justine Nuytens (UGent) , Eveline Vanden Eynde (UGent) , et al.
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Abstract
Long non-coding RNAs (lncRNAs) can exhibit cell-type and cancer-type specific expression profiles, making them highly attractive as therapeutic targets. Pan-cancer RNA sequencing data revealed broad expression of the SAMMSON lncRNA in uveal melanoma (UM), the most common primary intraocular malignancy in adults. Currently, there are no effective treatments for UM patients with metastatic disease, resulting in a median survival time of 6-12 months. We aimed to investigate the therapeutic potential of SAMMSON inhibition in UM. Antisense oligonucleotide (ASO)-mediated SAMMSON inhibition impaired the growth and viability of a genetically diverse panel of uveal melanoma cell lines. These effects were accompanied by an induction of apoptosis and were recapitulated in two uveal melanoma patient derived xenograft (PDX) models through subcutaneous ASO delivery. SAMMSON pulldown revealed several candidate interaction partners, including various proteins involved in mitochondrial translation. Consequently, inhibition of SAMMSON impaired global, mitochondrial and cytosolic protein translation levels and mitochondrial function in uveal melanoma cells. The present study demonstrates that SAMMSON expression is essential for uveal melanoma cell survival. ASO-mediated silencing of SAMMSON may provide an effective treatment strategy to treat primary and metastatic uveal melanoma patients.
Keywords
Cancer Research, Genetics, Molecular Biology, ESTABLISHMENT, GLYCYLCYCLINE, TIGECYCLINE, KI-67

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MLA
Dewaele, Shanna, et al. “The Long Non-Coding RNA SAMMSON Is Essential for Uveal Melanoma Cell Survival.” ONCOGENE, 2021, doi:10.1038/s41388-021-02006-x.
APA
Dewaele, S., Delhaye, L., De Paepe, B., de Bony de Lavergne, E., De Wilde, J., Vanderheyden, K., … Mestdagh, P. (2021). The long non-coding RNA SAMMSON is essential for uveal melanoma cell survival. ONCOGENE. https://doi.org/10.1038/s41388-021-02006-x
Chicago author-date
Dewaele, Shanna, Louis Delhaye, Boel De Paepe, Eric de Bony de Lavergne, Jilke De Wilde, Katrien Vanderheyden, Jasper Anckaert, et al. 2021. “The Long Non-Coding RNA SAMMSON Is Essential for Uveal Melanoma Cell Survival.” ONCOGENE. https://doi.org/10.1038/s41388-021-02006-x.
Chicago author-date (all authors)
Dewaele, Shanna, Louis Delhaye, Boel De Paepe, Eric de Bony de Lavergne, Jilke De Wilde, Katrien Vanderheyden, Jasper Anckaert, Nurten Yigit, Justine Nuytens, Eveline Vanden Eynde, Joél Smet, Maxime Verschoore, Fariba Nemati, Didier Decaudin, Manuel Rodrigues, Peihua Zhao, Aart Jochemsen, Eleonora Leucci, Jo Vandesompele, Jo Van Dorpe, Jean-Christophe Marine, Rudy Van Coster, Sven Eyckerman, and Pieter Mestdagh. 2021. “The Long Non-Coding RNA SAMMSON Is Essential for Uveal Melanoma Cell Survival.” ONCOGENE. doi:10.1038/s41388-021-02006-x.
Vancouver
1.
Dewaele S, Delhaye L, De Paepe B, de Bony de Lavergne E, De Wilde J, Vanderheyden K, et al. The long non-coding RNA SAMMSON is essential for uveal melanoma cell survival. ONCOGENE. 2021;
IEEE
[1]
S. Dewaele et al., “The long non-coding RNA SAMMSON is essential for uveal melanoma cell survival,” ONCOGENE, 2021.
@article{8720221,
  abstract     = {{Long non-coding RNAs (lncRNAs) can exhibit cell-type and cancer-type specific expression profiles, making them highly attractive as therapeutic targets. Pan-cancer RNA sequencing data revealed broad expression of the SAMMSON lncRNA in uveal melanoma (UM), the most common primary intraocular malignancy in adults. Currently, there are no effective treatments for UM patients with metastatic disease, resulting in a median survival time of 6-12 months. We aimed to investigate the therapeutic potential of SAMMSON inhibition in UM. Antisense oligonucleotide (ASO)-mediated SAMMSON inhibition impaired the growth and viability of a genetically diverse panel of uveal melanoma cell lines. These effects were accompanied by an induction of apoptosis and were recapitulated in two uveal melanoma patient derived xenograft (PDX) models through subcutaneous ASO delivery. SAMMSON pulldown revealed several candidate interaction partners, including various proteins involved in mitochondrial translation. Consequently, inhibition of SAMMSON impaired global, mitochondrial and cytosolic protein translation levels and mitochondrial function in uveal melanoma cells. The present study demonstrates that SAMMSON expression is essential for uveal melanoma cell survival. ASO-mediated silencing of SAMMSON may provide an effective treatment strategy to treat primary and metastatic uveal melanoma patients.}},
  author       = {{Dewaele, Shanna and Delhaye, Louis and De Paepe, Boel and de Bony de Lavergne, Eric and De Wilde, Jilke and Vanderheyden, Katrien and Anckaert, Jasper and Yigit, Nurten and Nuytens, Justine and Vanden Eynde, Eveline and Smet, Joél and Verschoore, Maxime and Nemati, Fariba and Decaudin, Didier and Rodrigues, Manuel and Zhao, Peihua and Jochemsen, Aart and Leucci, Eleonora and Vandesompele, Jo and Van Dorpe, Jo and Marine, Jean-Christophe and Van Coster, Rudy and Eyckerman, Sven and Mestdagh, Pieter}},
  issn         = {{0950-9232}},
  journal      = {{ONCOGENE}},
  keywords     = {{Cancer Research,Genetics,Molecular Biology,ESTABLISHMENT,GLYCYLCYCLINE,TIGECYCLINE,KI-67}},
  language     = {{eng}},
  pages        = {{11}},
  title        = {{The long non-coding RNA SAMMSON is essential for uveal melanoma cell survival}},
  url          = {{http://dx.doi.org/10.1038/s41388-021-02006-x}},
  year         = {{2021}},
}

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