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Lignin aromatics to PHA polymers : nitrogen and oxygen are the key factors for pseudomonas

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Abstract
Many wild type Pseudomonas strains have the potential to contribute to the valorization of lignin in future biorefineries. Through a robust aromatic catabolism, i.e., biofunneling capacity, they can ease the inherent aromatic heterogeneity found in lignin hydrolysates and accumulate naturally marketable biopolymers like mcl-polyhydroxyalkanoate (mcl-PHA) under nitrogen limitation. Besides a comparative strain evaluation, we present fundamental research on the funneling of aromatic mixtures under specific bioprocess conditions to improve biocatalytic lignin valorization. For the most robust and best performing strain, P. putida KT2440, we improve the mcl-PHA accumulation from a defined aromatic mixture of p-coumarate, ferulate, and benzoate under technically relevant conditions by up to 40% by tailoring the nitrogen and oxygen supply. The highest mcl-PHA concentration (582 +/- 41 mg L-1) was obtained for a C/N ratio of 60 for oxygen-unlimited conditions (oxygen transfer rate >20 mmol L-1 h(-1)). In contrast, aromatic intermediates accumulated under oxygen-limited conditions at oxygen transfer rates below 10 mmol L-1 h(-1). The experimental conditions were scalable into a 1L stirred tank bioreactor. This study contributes to deepening our understanding of the biocatalytic capability of promising Pseudomonas strains toward downstream microbial conversions of lignin aromatics for future biorefinery applications.
Keywords
Pseudomonas putida KT2440, Lignin valorization, Biological funnelling, Biorefining, Bioprocess development, Aromatic mixture, POLYHYDROXYALKANOATE PHA, PUTIDA KT2440, DEPOLYMERIZATION, VALORIZATION, CHEMICALS, QUANTIFICATION, BIOSYNTHESIS, LIMITATIONS, METABOLISM, REPRESSION

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MLA
Ramirez Morales, Juan Esteban, et al. “Lignin Aromatics to PHA Polymers : Nitrogen and Oxygen Are the Key Factors for Pseudomonas.” ACS SUSTAINABLE CHEMISTRY & ENGINEERING, vol. 9, no. 31, 2021, pp. 10579–90, doi:10.1021/acssuschemeng.1c02682.
APA
Ramirez Morales, J. E., Czichowski, P., Besirlioglu, V., Regestein, L., Rabaey, K., Blank, L. M., & Rosenbaum, M. A. (2021). Lignin aromatics to PHA polymers : nitrogen and oxygen are the key factors for pseudomonas. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 9(31), 10579–10590. https://doi.org/10.1021/acssuschemeng.1c02682
Chicago author-date
Ramirez Morales, Juan Esteban, Phillip Czichowski, Volkan Besirlioglu, Lars Regestein, Korneel Rabaey, Lars M. Blank, and Miriam A. Rosenbaum. 2021. “Lignin Aromatics to PHA Polymers : Nitrogen and Oxygen Are the Key Factors for Pseudomonas.” ACS SUSTAINABLE CHEMISTRY & ENGINEERING 9 (31): 10579–90. https://doi.org/10.1021/acssuschemeng.1c02682.
Chicago author-date (all authors)
Ramirez Morales, Juan Esteban, Phillip Czichowski, Volkan Besirlioglu, Lars Regestein, Korneel Rabaey, Lars M. Blank, and Miriam A. Rosenbaum. 2021. “Lignin Aromatics to PHA Polymers : Nitrogen and Oxygen Are the Key Factors for Pseudomonas.” ACS SUSTAINABLE CHEMISTRY & ENGINEERING 9 (31): 10579–10590. doi:10.1021/acssuschemeng.1c02682.
Vancouver
1.
Ramirez Morales JE, Czichowski P, Besirlioglu V, Regestein L, Rabaey K, Blank LM, et al. Lignin aromatics to PHA polymers : nitrogen and oxygen are the key factors for pseudomonas. ACS SUSTAINABLE CHEMISTRY & ENGINEERING. 2021;9(31):10579–90.
IEEE
[1]
J. E. Ramirez Morales et al., “Lignin aromatics to PHA polymers : nitrogen and oxygen are the key factors for pseudomonas,” ACS SUSTAINABLE CHEMISTRY & ENGINEERING, vol. 9, no. 31, pp. 10579–10590, 2021.
@article{8719174,
  abstract     = {{Many wild type Pseudomonas strains have the potential to contribute to the valorization of lignin in future biorefineries. Through a robust aromatic catabolism, i.e., biofunneling capacity, they can ease the inherent aromatic heterogeneity found in lignin hydrolysates and accumulate naturally marketable biopolymers like mcl-polyhydroxyalkanoate (mcl-PHA) under nitrogen limitation. Besides a comparative strain evaluation, we present fundamental research on the funneling of aromatic mixtures under specific bioprocess conditions to improve biocatalytic lignin valorization. For the most robust and best performing strain, P. putida KT2440, we improve the mcl-PHA accumulation from a defined aromatic mixture of p-coumarate, ferulate, and benzoate under technically relevant conditions by up to 40% by tailoring the nitrogen and oxygen supply. The highest mcl-PHA concentration (582 +/- 41 mg L-1) was obtained for a C/N ratio of 60 for oxygen-unlimited conditions (oxygen transfer rate >20 mmol L-1 h(-1)). In contrast, aromatic intermediates accumulated under oxygen-limited conditions at oxygen transfer rates below 10 mmol L-1 h(-1). The experimental conditions were scalable into a 1L stirred tank bioreactor. This study contributes to deepening our understanding of the biocatalytic capability of promising Pseudomonas strains toward downstream microbial conversions of lignin aromatics for future biorefinery applications.}},
  author       = {{Ramirez Morales, Juan Esteban and Czichowski, Phillip and Besirlioglu, Volkan and Regestein, Lars and Rabaey, Korneel and Blank, Lars M. and Rosenbaum, Miriam A.}},
  issn         = {{2168-0485}},
  journal      = {{ACS SUSTAINABLE CHEMISTRY & ENGINEERING}},
  keywords     = {{Pseudomonas putida KT2440,Lignin valorization,Biological funnelling,Biorefining,Bioprocess development,Aromatic mixture,POLYHYDROXYALKANOATE PHA,PUTIDA KT2440,DEPOLYMERIZATION,VALORIZATION,CHEMICALS,QUANTIFICATION,BIOSYNTHESIS,LIMITATIONS,METABOLISM,REPRESSION}},
  language     = {{eng}},
  number       = {{31}},
  pages        = {{10579--10590}},
  title        = {{Lignin aromatics to PHA polymers : nitrogen and oxygen are the key factors for pseudomonas}},
  url          = {{http://dx.doi.org/10.1021/acssuschemeng.1c02682}},
  volume       = {{9}},
  year         = {{2021}},
}

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