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Immunomodulatory activity of electrospun polyhydroxyalkanoate fiber scaffolds incorporating olive leaf extract

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Abstract
Olive tree is a well-known source of polyphenols. We prepared an olive leaf extract (OLE) and characterized it via high performance liquid chromatography (HPLC) analysis. OLE was blended with different polyhydroxyalkanoates (PHAs), namely, poly(hydroxybutyrate-co-hydroxyvalerate) (PHBHV) and polyhydroxybutyrate/poly(hydroxyoctanoate-co-hydroxydecanoate) (PHB/PHOHD), to produce fiber meshes via electrospinning: OLE/PHBV and OLE/ (PHB/PHOHD), respectively. An 80–90% (w/w%) release of the main polyphenols from the OLE/PHA fibers occurred in 24 h, with a burst release in the first 30 min. OLE and the produced fiber meshes were assayed using human dermal keratinocytes (HaCaT cells) to evaluate the expression of a panel of cytokines involved in the inflammatory process and innate immune response, such as the antimicrobial peptide human beta defensin 2 (HBD-2). Fibers containing OLE were able to decrease the expression of the pro-inflammatory cytokines at 6 h up to 24 h. All the PHA fibers allowed an early downregulation of the pro-inflammatory cytokines in 6 h, which is suggestive of a strong anti-inflammatory activity exerted by PHA fibers. Differently from pure OLE, PHB/PHOHD fibers (both with and without OLE) upregulated the expression of HBD-2. Our results showed that PHA fiber meshes are suitable in decreasing pro-inflammatory cytokines and the incorporation of OLE may enable indirect antibacterial properties, which is essential in wound healing and tissue regeneration.
Keywords
polyphenol, anti-inflammatory, antibacterial, wound healing, wound dressings, tissue regeneration, bio-based

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MLA
De la Ossa, Jose Gustavo, et al. “Immunomodulatory Activity of Electrospun Polyhydroxyalkanoate Fiber Scaffolds Incorporating Olive Leaf Extract.” APPLIED SCIENCES-BASEL, vol. 11, no. 9, 2021, doi:10.3390/app11094006.
APA
De la Ossa, J. G., Fusco, A., Azimi, B., Esposito Salsano, J., Digiacomo, M., Coltelli, M.-B., … Di Stefano, R. (2021). Immunomodulatory activity of electrospun polyhydroxyalkanoate fiber scaffolds incorporating olive leaf extract. APPLIED SCIENCES-BASEL, 11(9). https://doi.org/10.3390/app11094006
Chicago author-date
De la Ossa, Jose Gustavo, Alessandra Fusco, Bahareh Azimi, Jasmine Esposito Salsano, Maria Digiacomo, Maria-Beatrice Coltelli, Karen De Clerck, et al. 2021. “Immunomodulatory Activity of Electrospun Polyhydroxyalkanoate Fiber Scaffolds Incorporating Olive Leaf Extract.” APPLIED SCIENCES-BASEL 11 (9). https://doi.org/10.3390/app11094006.
Chicago author-date (all authors)
De la Ossa, Jose Gustavo, Alessandra Fusco, Bahareh Azimi, Jasmine Esposito Salsano, Maria Digiacomo, Maria-Beatrice Coltelli, Karen De Clerck, Ipsita Roy, Marco Macchia, Andrea Lazzeri, Giovanna Donnarumma, Serena Danti, and Rossella Di Stefano. 2021. “Immunomodulatory Activity of Electrospun Polyhydroxyalkanoate Fiber Scaffolds Incorporating Olive Leaf Extract.” APPLIED SCIENCES-BASEL 11 (9). doi:10.3390/app11094006.
Vancouver
1.
De la Ossa JG, Fusco A, Azimi B, Esposito Salsano J, Digiacomo M, Coltelli M-B, et al. Immunomodulatory activity of electrospun polyhydroxyalkanoate fiber scaffolds incorporating olive leaf extract. APPLIED SCIENCES-BASEL. 2021;11(9).
IEEE
[1]
J. G. De la Ossa et al., “Immunomodulatory activity of electrospun polyhydroxyalkanoate fiber scaffolds incorporating olive leaf extract,” APPLIED SCIENCES-BASEL, vol. 11, no. 9, 2021.
@article{8719144,
  abstract     = {{Olive tree is a well-known source of polyphenols. We prepared an olive leaf extract (OLE) and characterized it via high performance liquid chromatography (HPLC) analysis. OLE was blended with different polyhydroxyalkanoates (PHAs), namely, poly(hydroxybutyrate-co-hydroxyvalerate) (PHBHV) and polyhydroxybutyrate/poly(hydroxyoctanoate-co-hydroxydecanoate) (PHB/PHOHD), to produce fiber meshes via electrospinning: OLE/PHBV and OLE/ (PHB/PHOHD), respectively. An 80–90% (w/w%) release of the main polyphenols from the OLE/PHA fibers occurred in 24 h, with a burst release in the first 30 min. OLE and the produced fiber meshes were assayed using human dermal keratinocytes (HaCaT cells) to evaluate the expression of a panel of cytokines involved in the inflammatory process and innate immune response, such as the antimicrobial peptide human beta defensin 2 (HBD-2). Fibers containing OLE were able to decrease the expression of the pro-inflammatory cytokines at 6 h up to 24 h. All the PHA fibers allowed an early downregulation of the pro-inflammatory cytokines in 6 h, which is suggestive of a strong anti-inflammatory activity exerted by PHA fibers. Differently from pure OLE, PHB/PHOHD fibers (both with and without OLE) upregulated the expression of HBD-2. Our results showed that PHA fiber meshes are suitable in decreasing pro-inflammatory cytokines and the incorporation of OLE may enable indirect antibacterial properties, which is essential in wound healing and tissue regeneration.}},
  articleno    = {{4006}},
  author       = {{De la Ossa, Jose Gustavo and Fusco, Alessandra and Azimi, Bahareh and Esposito Salsano, Jasmine and Digiacomo, Maria and Coltelli, Maria-Beatrice and De Clerck, Karen and Roy, Ipsita and Macchia, Marco and Lazzeri, Andrea and Donnarumma, Giovanna and Danti, Serena and Di Stefano, Rossella}},
  issn         = {{2076-3417}},
  journal      = {{APPLIED SCIENCES-BASEL}},
  keywords     = {{polyphenol,anti-inflammatory,antibacterial,wound healing,wound dressings,tissue regeneration,bio-based}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{15}},
  title        = {{Immunomodulatory activity of electrospun polyhydroxyalkanoate fiber scaffolds incorporating olive leaf extract}},
  url          = {{http://dx.doi.org/10.3390/app11094006}},
  volume       = {{11}},
  year         = {{2021}},
}

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