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Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm

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Abstract
Previous research identified potential prebiotic substrates for oral health like the structural analogues N-acetyl-d-mannosamine (NADM) and N-acetyl-d-glucosamine (NADG). The main hypothesis of the current study was twofold. Firstly, it was hypothesized that the modulatory effects of NADM are not limited to changes in multi-species oral biofilm composition, but also include effects on metabolism, virulence, and inflammatory potential. Secondly, the presence and orientation of their N-acetyl group could play a role. Therefore, a comparison was made between the effects of NADM, NADG and d-(+)-mannose on multi-species oral biofilms. Besides a beneficial compositional shift, NADM-treated biofilms also showed an altered metabolism, a reduced virulence and a decreased inflammatory potential. At a substrate concentration of 1 M, these effects were pronounced for all biofilm aspects, whereas at similar to 0.05 M (1%((w/v))) only the effects on virulence were pronounced. When comparing between substrates, both the presence and orientation of the N-acetyl group played a role. However, this was generally only at 1 M and dependent on the biofilm aspect. Overall, NADM was found to have different effects at two concentrations that beneficially modulate in vitro multi-species oral biofilm composition, metabolism, virulence and inflammatory potential. The presence and orientation of the N-acetyl group influenced these effects.
Keywords
INTERNATIONAL SCIENTIFIC ASSOCIATION, N-ACETYLNEURAMINIC ACID, SUBINHIBITORY CONCENTRATIONS, POLYMICROBIAL SYNERGY, CONSENSUS STATEMENT, PERIODONTAL-DISEASE, GENE-EXPRESSION, MICROBIOME, PROBIOTICS, ACETYLMANNOSAMINE

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MLA
Verspecht, Tim, et al. “Comparison of the Modulatory Effects of Three Structurally Similar Potential Prebiotic Substrates on an in Vitro Multi-Species Oral Biofilm.” SCIENTIFIC REPORTS, vol. 11, no. 1, 2021, doi:10.1038/s41598-021-94510-z.
APA
Verspecht, T., Van Holm, W., Boon, N., Bernaerts, K., Daep, C. A., Zayed, N., … Teughels, W. (2021). Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm. SCIENTIFIC REPORTS, 11(1). https://doi.org/10.1038/s41598-021-94510-z
Chicago author-date
Verspecht, Tim, Wannes Van Holm, Nico Boon, Kristel Bernaerts, Carlo A. Daep, Naiera Zayed, Marc Quirynen, and Wim Teughels. 2021. “Comparison of the Modulatory Effects of Three Structurally Similar Potential Prebiotic Substrates on an in Vitro Multi-Species Oral Biofilm.” SCIENTIFIC REPORTS 11 (1). https://doi.org/10.1038/s41598-021-94510-z.
Chicago author-date (all authors)
Verspecht, Tim, Wannes Van Holm, Nico Boon, Kristel Bernaerts, Carlo A. Daep, Naiera Zayed, Marc Quirynen, and Wim Teughels. 2021. “Comparison of the Modulatory Effects of Three Structurally Similar Potential Prebiotic Substrates on an in Vitro Multi-Species Oral Biofilm.” SCIENTIFIC REPORTS 11 (1). doi:10.1038/s41598-021-94510-z.
Vancouver
1.
Verspecht T, Van Holm W, Boon N, Bernaerts K, Daep CA, Zayed N, et al. Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm. SCIENTIFIC REPORTS. 2021;11(1).
IEEE
[1]
T. Verspecht et al., “Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm,” SCIENTIFIC REPORTS, vol. 11, no. 1, 2021.
@article{8718485,
  abstract     = {{Previous research identified potential prebiotic substrates for oral health like the structural analogues N-acetyl-d-mannosamine (NADM) and N-acetyl-d-glucosamine (NADG). The main hypothesis of the current study was twofold. Firstly, it was hypothesized that the modulatory effects of NADM are not limited to changes in multi-species oral biofilm composition, but also include effects on metabolism, virulence, and inflammatory potential. Secondly, the presence and orientation of their N-acetyl group could play a role. Therefore, a comparison was made between the effects of NADM, NADG and d-(+)-mannose on multi-species oral biofilms. Besides a beneficial compositional shift, NADM-treated biofilms also showed an altered metabolism, a reduced virulence and a decreased inflammatory potential. At a substrate concentration of 1 M, these effects were pronounced for all biofilm aspects, whereas at similar to 0.05 M (1%((w/v))) only the effects on virulence were pronounced. When comparing between substrates, both the presence and orientation of the N-acetyl group played a role. However, this was generally only at 1 M and dependent on the biofilm aspect. Overall, NADM was found to have different effects at two concentrations that beneficially modulate in vitro multi-species oral biofilm composition, metabolism, virulence and inflammatory potential. The presence and orientation of the N-acetyl group influenced these effects.}},
  articleno    = {{15033}},
  author       = {{Verspecht, Tim and Van Holm, Wannes and Boon, Nico and Bernaerts, Kristel and Daep, Carlo A. and Zayed, Naiera and Quirynen, Marc and Teughels, Wim}},
  issn         = {{2045-2322}},
  journal      = {{SCIENTIFIC REPORTS}},
  keywords     = {{INTERNATIONAL SCIENTIFIC ASSOCIATION,N-ACETYLNEURAMINIC ACID,SUBINHIBITORY CONCENTRATIONS,POLYMICROBIAL SYNERGY,CONSENSUS STATEMENT,PERIODONTAL-DISEASE,GENE-EXPRESSION,MICROBIOME,PROBIOTICS,ACETYLMANNOSAMINE}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{15}},
  title        = {{Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm}},
  url          = {{http://dx.doi.org/10.1038/s41598-021-94510-z}},
  volume       = {{11}},
  year         = {{2021}},
}

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