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Acute preexercise supplementation of combined carnosine and anserine enhances initial maximal power of Wingate tests in humans

Laura Blancquaert (UGent) , Inge Everaert (UGent) , Audrey Baguet (UGent) , Tine Bex (UGent) , Silvia Barbaresi (UGent) , Sarah de Jager (UGent) , Eline Lievens (UGent) , Jan Stautemas (UGent) , S. De Smet, G. Baron, et al.
(2021) JOURNAL OF APPLIED PHYSIOLOGY. 130(6). p.1868-1878
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Abstract
Classic in vitro experiments (Severin's phenomenon) demonstrated that acute carnosine supplementation may potentiate muscle contractility. However, upon oral ingestion, carnosine is readily degraded in human plasma by the highly active serum carnosinase-1 (CN1). We developed a novel strategy to circumvent CN1 by preexercise ingestion of combined carnosine (CARN) and anserine (ANS), the methylated analog with similar biochemical properties but more resistant to CN1. First, in vitro hydrolysis was tested by adding carnosine and anserine to human plasma, alone or in combination. Second, five subjects were supplemented with 25 mg/kg anserine or 25 mg/kg of each anserine and carnosine to test in vivo bioavailability. Third, two double-blind, placebo-controlled, crossover studies investigated the effect of preexercise ANS + CARN (20 mg/kg body wt of each) supplementation on performance during a single all-out Wingate test following 6-min high-intensity cycling (study A) or three repeated Wingate tests (study B). In vitro experiments demonstrated slower degradation of anserine versus carnosine, which was further slowed by simultaneously adding carnosine. In vivo bioavailability of plasma anserine was more prominent [2.5-fold increased area under the curve (AUC)] when ANS + CARN versus ANS was ingested. Study A showed significantly higher (+ 6%+/- 11%; P = 0.04) power in the first 5s of the Wingate test following ANS + CARN (12.8 +/- 2.4 W/kg) versus placebo (12.1 +/- 2.2 W/kg). Study B demonstrated increased peak power (+ 3%) throughout three consecutive Wingate tests (ANS + CARN 10.5 +/- 0.6 W/kg vs. placebo 10.2 +/- 9.9W/kg). These experiments reveal a novel acute nutritional method to effectively raise plasma anserine and carnosine by high-dose combined supplementation. This approach led to improved initial cycling power, revealing a new nutritional strategy to increase exercise performance. NEW & NOTEWORTHY Current results reveal that carnosine and anserine competitively bind to the highly active carnosinase enzyme in human plasma. Acute combined carnosine and anserine supplementation is therefore described as novel strategy to raise plasma anserine and carnosine. We report that indices of maximal exercise/muscle power during the initial stage of a Wingate test were significantly improved by preexercise 20-25mg/kg body wt anserine and carnosine supplementation, pointing toward a novel acute nutritional strategy to improve high-intensity exercise performance.
Keywords
Physiology (medical), Physiology, acute nutritional strategy, ergogenic supplement, high-intensity exercise, serum carnosinase-1, BETA-ALANINE SUPPLEMENTATION, PERFORMANCE LIQUID-CHROMATOGRAPHY, INTESTINAL-ABSORPTION, MUSCLE, INGESTION, GLUTATHIONE

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MLA
Blancquaert, Laura, et al. “Acute Preexercise Supplementation of Combined Carnosine and Anserine Enhances Initial Maximal Power of Wingate Tests in Humans.” JOURNAL OF APPLIED PHYSIOLOGY, vol. 130, no. 6, 2021, pp. 1868–78, doi:10.1152/japplphysiol.00602.2020.
APA
Blancquaert, L., Everaert, I., Baguet, A., Bex, T., Barbaresi, S., de Jager, S., … Derave, W. (2021). Acute preexercise supplementation of combined carnosine and anserine enhances initial maximal power of Wingate tests in humans. JOURNAL OF APPLIED PHYSIOLOGY, 130(6), 1868–1878. https://doi.org/10.1152/japplphysiol.00602.2020
Chicago author-date
Blancquaert, Laura, Inge Everaert, Audrey Baguet, Tine Bex, Silvia Barbaresi, Sarah de Jager, Eline Lievens, et al. 2021. “Acute Preexercise Supplementation of Combined Carnosine and Anserine Enhances Initial Maximal Power of Wingate Tests in Humans.” JOURNAL OF APPLIED PHYSIOLOGY 130 (6): 1868–78. https://doi.org/10.1152/japplphysiol.00602.2020.
Chicago author-date (all authors)
Blancquaert, Laura, Inge Everaert, Audrey Baguet, Tine Bex, Silvia Barbaresi, Sarah de Jager, Eline Lievens, Jan Stautemas, S. De Smet, G. Baron, E. Gilardoni, L. Regazzoni, G. Aldini, and Wim Derave. 2021. “Acute Preexercise Supplementation of Combined Carnosine and Anserine Enhances Initial Maximal Power of Wingate Tests in Humans.” JOURNAL OF APPLIED PHYSIOLOGY 130 (6): 1868–1878. doi:10.1152/japplphysiol.00602.2020.
Vancouver
1.
Blancquaert L, Everaert I, Baguet A, Bex T, Barbaresi S, de Jager S, et al. Acute preexercise supplementation of combined carnosine and anserine enhances initial maximal power of Wingate tests in humans. JOURNAL OF APPLIED PHYSIOLOGY. 2021;130(6):1868–78.
IEEE
[1]
L. Blancquaert et al., “Acute preexercise supplementation of combined carnosine and anserine enhances initial maximal power of Wingate tests in humans,” JOURNAL OF APPLIED PHYSIOLOGY, vol. 130, no. 6, pp. 1868–1878, 2021.
@article{8717854,
  abstract     = {{Classic in vitro experiments (Severin's phenomenon) demonstrated that acute carnosine supplementation may potentiate muscle contractility. However, upon oral ingestion, carnosine is readily degraded in human plasma by the highly active serum carnosinase-1 (CN1). We developed a novel strategy to circumvent CN1 by preexercise ingestion of combined carnosine (CARN) and anserine (ANS), the methylated analog with similar biochemical properties but more resistant to CN1. First, in vitro hydrolysis was tested by adding carnosine and anserine to human plasma, alone or in combination. Second, five subjects were supplemented with 25 mg/kg anserine or 25 mg/kg of each anserine and carnosine to test in vivo bioavailability. Third, two double-blind, placebo-controlled, crossover studies investigated the effect of preexercise ANS + CARN (20 mg/kg body wt of each) supplementation on performance during a single all-out Wingate test following 6-min high-intensity cycling (study A) or three repeated Wingate tests (study B). In vitro experiments demonstrated slower degradation of anserine versus carnosine, which was further slowed by simultaneously adding carnosine. In vivo bioavailability of plasma anserine was more prominent [2.5-fold increased area under the curve (AUC)] when ANS + CARN versus ANS was ingested. Study A showed significantly higher (+ 6%+/- 11%; P = 0.04) power in the first 5s of the Wingate test following ANS + CARN (12.8 +/- 2.4 W/kg) versus placebo (12.1 +/- 2.2 W/kg). Study B demonstrated increased peak power (+ 3%) throughout three consecutive Wingate tests (ANS + CARN 10.5 +/- 0.6 W/kg vs. placebo 10.2 +/- 9.9W/kg). These experiments reveal a novel acute nutritional method to effectively raise plasma anserine and carnosine by high-dose combined supplementation. This approach led to improved initial cycling power, revealing a new nutritional strategy to increase exercise performance.

NEW & NOTEWORTHY Current results reveal that carnosine and anserine competitively bind to the highly active carnosinase enzyme in human plasma. Acute combined carnosine and anserine supplementation is therefore described as novel strategy to raise plasma anserine and carnosine. We report that indices of maximal exercise/muscle power during the initial stage of a Wingate test were significantly improved by preexercise 20-25mg/kg body wt anserine and carnosine supplementation, pointing toward a novel acute nutritional strategy to improve high-intensity exercise performance.}},
  author       = {{Blancquaert, Laura and Everaert, Inge and Baguet, Audrey and Bex, Tine and Barbaresi, Silvia and de Jager, Sarah and Lievens, Eline and Stautemas, Jan and De Smet, S. and Baron, G. and Gilardoni, E. and Regazzoni, L. and Aldini, G. and Derave, Wim}},
  issn         = {{8750-7587}},
  journal      = {{JOURNAL OF APPLIED PHYSIOLOGY}},
  keywords     = {{Physiology (medical),Physiology,acute nutritional strategy,ergogenic supplement,high-intensity exercise,serum carnosinase-1,BETA-ALANINE SUPPLEMENTATION,PERFORMANCE LIQUID-CHROMATOGRAPHY,INTESTINAL-ABSORPTION,MUSCLE,INGESTION,GLUTATHIONE}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{1868--1878}},
  title        = {{Acute preexercise supplementation of combined carnosine and anserine enhances initial maximal power of Wingate tests in humans}},
  url          = {{http://dx.doi.org/10.1152/japplphysiol.00602.2020}},
  volume       = {{130}},
  year         = {{2021}},
}

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