Advanced search
Add to list

Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry

Author
Organization
Abstract
Of the many metabolites involved in any clinical condition, only a narrow range of biomarkers is currently being used in the clinical setting. A key to personalized medicine would be to extend this range. Metabolic fingerprinting provides a more comprehensive insight, but many methods used for metabolomics analysis are too complex and time-consuming to be diagnostically useful. Here, a rapid evaporative ionization mass spectrometry (REIMS) system for direct ex vivo real-time analysis of biofluids with minor sample pretreatment is detailed. The REIMS can be linked to various laser wavelength systems (such as optical parametric oscillator or CO2 laser) and with automation for high-throughput analysis. Laser-induced sample evaporation occurs within seconds through radiative heating with the plume guided to the MS instrument. The presented procedure includes (i) laser setup with automation, (ii) analysis of biofluids (blood/urine/stool/saliva/sputum/breast milk) and (iii) data analysis. We provide the optimal settings for biofluid analysis and quality control, enabling sensitive, precise and robust analysis. Using the automated setup, 96 samples can be analyzed in similar to 35-40 min per ionization mode, with no intervention required. Metabolic fingerprints are made up of 2,000-4,000 features, for which relative quantification can be achieved at high repeatability when total ion current normalization is applied. With saliva and feces as example matrices, >70% of features had a coefficient of variance <= 30%. However, to achieve acceptable long-term reproducibility, additional normalizations by, e.g., LOESS are recommended, especially for positive ionization.
Keywords
PERFORMANCE LIQUID-CHROMATOGRAPHY, IN-SITU, STORAGE-CONDITIONS, HUMAN-MILK, METABOLOMICS, IMPACT, TISSUE, PLATFORM, REVEALS, SAMPLES

Citation

Please use this url to cite or link to this publication:

MLA
Plekhova, Vera, et al. “Rapid Ex Vivo Molecular Fingerprinting of Biofluids Using Laser-Assisted Rapid Evaporative Ionization Mass Spectrometry.” NATURE PROTOCOLS, 2021, doi:10.1038/s41596-021-00580-8.
APA
Plekhova, V., Van Meulebroek, L., De Graeve, M., Perdones-Montero, A., De Spiegeleer, M., De Paepe, E., … Vanhaecke, L. (2021). Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry. NATURE PROTOCOLS. https://doi.org/10.1038/s41596-021-00580-8
Chicago author-date
Plekhova, Vera, Lieven Van Meulebroek, Marilyn De Graeve, Alvaro Perdones-Montero, Margot De Spiegeleer, Ellen De Paepe, Emma Van de Walle, Zoltan Takats, Simon J. S. Cameron, and Lynn Vanhaecke. 2021. “Rapid Ex Vivo Molecular Fingerprinting of Biofluids Using Laser-Assisted Rapid Evaporative Ionization Mass Spectrometry.” NATURE PROTOCOLS. https://doi.org/10.1038/s41596-021-00580-8.
Chicago author-date (all authors)
Plekhova, Vera, Lieven Van Meulebroek, Marilyn De Graeve, Alvaro Perdones-Montero, Margot De Spiegeleer, Ellen De Paepe, Emma Van de Walle, Zoltan Takats, Simon J. S. Cameron, and Lynn Vanhaecke. 2021. “Rapid Ex Vivo Molecular Fingerprinting of Biofluids Using Laser-Assisted Rapid Evaporative Ionization Mass Spectrometry.” NATURE PROTOCOLS. doi:10.1038/s41596-021-00580-8.
Vancouver
1.
Plekhova V, Van Meulebroek L, De Graeve M, Perdones-Montero A, De Spiegeleer M, De Paepe E, et al. Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry. NATURE PROTOCOLS. 2021;
IEEE
[1]
V. Plekhova et al., “Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry,” NATURE PROTOCOLS, 2021.
@article{8717479,
  abstract     = {{Of the many metabolites involved in any clinical condition, only a narrow range of biomarkers is currently being used in the clinical setting. A key to personalized medicine would be to extend this range. Metabolic fingerprinting provides a more comprehensive insight, but many methods used for metabolomics analysis are too complex and time-consuming to be diagnostically useful. Here, a rapid evaporative ionization mass spectrometry (REIMS) system for direct ex vivo real-time analysis of biofluids with minor sample pretreatment is detailed. The REIMS can be linked to various laser wavelength systems (such as optical parametric oscillator or CO2 laser) and with automation for high-throughput analysis. Laser-induced sample evaporation occurs within seconds through radiative heating with the plume guided to the MS instrument. The presented procedure includes (i) laser setup with automation, (ii) analysis of biofluids (blood/urine/stool/saliva/sputum/breast milk) and (iii) data analysis. We provide the optimal settings for biofluid analysis and quality control, enabling sensitive, precise and robust analysis. Using the automated setup, 96 samples can be analyzed in similar to 35-40 min per ionization mode, with no intervention required. Metabolic fingerprints are made up of 2,000-4,000 features, for which relative quantification can be achieved at high repeatability when total ion current normalization is applied. With saliva and feces as example matrices, >70% of features had a coefficient of variance <= 30%. However, to achieve acceptable long-term reproducibility, additional normalizations by, e.g., LOESS are recommended, especially for positive ionization.}},
  author       = {{Plekhova, Vera and Van Meulebroek, Lieven and De Graeve, Marilyn and Perdones-Montero, Alvaro and De Spiegeleer, Margot and De Paepe, Ellen and Van de Walle, Emma and Takats, Zoltan and Cameron, Simon J. S. and Vanhaecke, Lynn}},
  issn         = {{1754-2189}},
  journal      = {{NATURE PROTOCOLS}},
  keywords     = {{PERFORMANCE LIQUID-CHROMATOGRAPHY,IN-SITU,STORAGE-CONDITIONS,HUMAN-MILK,METABOLOMICS,IMPACT,TISSUE,PLATFORM,REVEALS,SAMPLES}},
  language     = {{eng}},
  pages        = {{31}},
  title        = {{Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry}},
  url          = {{http://dx.doi.org/10.1038/s41596-021-00580-8}},
  year         = {{2021}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: