Cell surface and intracellular auxin signalling for H+ fluxes in root growth
- Author
- Lanxin Li, Inge Verstraeten (UGent) , Mark Roosjen, Koji Takahashi, Lesia Rodriguez, Jack Merrin, Jian Chen (UGent) , Lana Shabala, Wouter Smet (UGent) , Hong Ren, Steffen Vanneste (UGent) , Sergey Shabala, Bert De Rybel (UGent) , Dolf Weijers, Toshinori Kinoshita, William M. Gray and Jiri Friml
- Organization
- Abstract
- Growth regulation tailors plant development to its environment. A showcase is adaptation to gravity, where shoots bend up and roots down. This paradox is based on opposite responses to the phytohormone auxin, which promotes cell expansion in shoots, while inhibiting it in roots via a yet unknown cellular mechanism. Here, by combining microfluidics, live imaging, genetic engineering and phospho-proteomics in Arabidopsis thaliana, we advance our understanding how auxin inhibits root growth. We show that auxin activates two distinct, antagonistically acting signalling pathways that converge on the rapid regulation of the apoplastic pH, which directly determines growth. Cell surface-based TRANSMEMBRANE KINASE 1 (TMK1) interacts with and mediates phosphorylation and activation of plasma membrane H+-ATPases for apoplast acidification, while intracellular TIR1/AFB-mediated signalling triggers net cellular H+-influx, causing apoplast alkalinisation. The simultaneous activation of these two counteracting mechanisms poises the root for a rapid, fine-tuned growth modulation to subtle changes in the environment.
- Keywords
- GENE, ATPASE, PHOSPHORYLATION, INHIBITION, ENDOCYTOSIS, ELONGATION, EXPANSION, PLANTS, FAMILY, CA2+
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8717354
- MLA
- Li, Lanxin, et al. “Cell Surface and Intracellular Auxin Signalling for H+ Fluxes in Root Growth.” NATURE, vol. 599, no. 7884, 2021, pp. 273–77, doi:10.1038/s41586-021-04037-6.
- APA
- Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez, L., Merrin, J., … Friml, J. (2021). Cell surface and intracellular auxin signalling for H+ fluxes in root growth. NATURE, 599(7884), 273–277. https://doi.org/10.1038/s41586-021-04037-6
- Chicago author-date
- Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez, Jack Merrin, Jian Chen, et al. 2021. “Cell Surface and Intracellular Auxin Signalling for H+ Fluxes in Root Growth.” NATURE 599 (7884): 273–77. https://doi.org/10.1038/s41586-021-04037-6.
- Chicago author-date (all authors)
- Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez, Jack Merrin, Jian Chen, Lana Shabala, Wouter Smet, Hong Ren, Steffen Vanneste, Sergey Shabala, Bert De Rybel, Dolf Weijers, Toshinori Kinoshita, William M. Gray, and Jiri Friml. 2021. “Cell Surface and Intracellular Auxin Signalling for H+ Fluxes in Root Growth.” NATURE 599 (7884): 273–277. doi:10.1038/s41586-021-04037-6.
- Vancouver
- 1.Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez L, Merrin J, et al. Cell surface and intracellular auxin signalling for H+ fluxes in root growth. NATURE. 2021;599(7884):273–7.
- IEEE
- [1]L. Li et al., “Cell surface and intracellular auxin signalling for H+ fluxes in root growth,” NATURE, vol. 599, no. 7884, pp. 273–277, 2021.
@article{8717354,
abstract = {{Growth regulation tailors plant development to its environment. A showcase is adaptation to gravity, where shoots bend up and roots down. This paradox is based on opposite responses to the phytohormone auxin, which promotes cell expansion in shoots, while inhibiting it in roots via a yet unknown cellular mechanism. Here, by combining microfluidics, live imaging, genetic engineering and phospho-proteomics in Arabidopsis thaliana, we advance our understanding how auxin inhibits root growth. We show that auxin activates two distinct, antagonistically acting signalling pathways that converge on the rapid regulation of the apoplastic pH, which directly determines growth. Cell surface-based TRANSMEMBRANE KINASE 1 (TMK1) interacts with and mediates phosphorylation and activation of plasma membrane H+-ATPases for apoplast acidification, while intracellular TIR1/AFB-mediated signalling triggers net cellular H+-influx, causing apoplast alkalinisation. The simultaneous activation of these two counteracting mechanisms poises the root for a rapid, fine-tuned growth modulation to subtle changes in the environment.}},
author = {{Li, Lanxin and Verstraeten, Inge and Roosjen, Mark and Takahashi, Koji and Rodriguez, Lesia and Merrin, Jack and Chen, Jian and Shabala, Lana and Smet, Wouter and Ren, Hong and Vanneste, Steffen and Shabala, Sergey and De Rybel, Bert and Weijers, Dolf and Kinoshita, Toshinori and Gray, William M. and Friml, Jiri}},
issn = {{0028-0836}},
journal = {{NATURE}},
keywords = {{GENE,ATPASE,PHOSPHORYLATION,INHIBITION,ENDOCYTOSIS,ELONGATION,EXPANSION,PLANTS,FAMILY,CA2+}},
language = {{eng}},
number = {{7884}},
pages = {{273--277}},
title = {{Cell surface and intracellular auxin signalling for H+ fluxes in root growth}},
url = {{http://doi.org/10.1038/s41586-021-04037-6}},
volume = {{599}},
year = {{2021}},
}
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