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Bacterial enrichment cultures biotransform the mycotoxin deoxynivalenol into a novel metabolite toxic to plant and porcine cells

Ilse Vanhoutte (UGent) , Caroline De Tender (UGent) , Kristel Demeyere (UGent) , Mohamed Fathi Abdallah (UGent) , Sarah Ommeslag, Pieter Vermeir (UGent) , Sarah De Saeger (UGent) , Jane Debode, Evelyne Meyer (UGent) , Siska Croubels (UGent) , et al.
(2021) TOXINS. 13(8).
Author
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Abstract
The mycotoxin deoxynivalenol (DON), produced in wheat, barley and maize by Fusarium graminearum and Fusarium culmorum, is threatening the health of humans and animals. With its worldwide high incidence in food and feed, mitigation strategies are needed to detoxify DON, maintaining the nutritional value and palatability of decontaminated commodities. A promising technique is biological degradation, where microorganisms are used to biotransform mycotoxins into less toxic metabolites. In this study, bacterial enrichment cultures were screened for their DON detoxification potential, where DON and its potential derivatives were monitored. The residual phytotoxicity was determined through a bioassay using the aquatic plant Lemna minor L. Two bacterial enrichment cultures were found to biotransform DON into a still highly toxic metabolite for plants. Furthermore, a cytotoxic effect was observed on the cellular viability of intestinal porcine epithelial cells. Through liquid chromatography high-resolution mass spectrometry analysis, an unknown compound was detected, and tentatively characterized with a molecular weight of 30.0 Da (i.e., CH2O) higher than DON. Metabarcoding of the subsequently enriched bacterial communities revealed a shift towards the genera Sphingopyxis, Pseudoxanthomonas, Ochrobactrum and Pseudarthrobacter. This work describes the discovery of a novel bacterial DON-derived metabolite, toxic to plant and porcine cells.
Keywords
Toxicology, Health, Toxicology and Mutagenesis, deoxynivalenol derivatives, enrichment, biotransformation, bioassay, cytotoxicity assay, metabarcoding, POLYCYCLIC AROMATIC-HYDROCARBONS, IN-VITRO, TRICHOTHECENE MYCOTOXINS, MICROBIAL-DEGRADATION, BIODEGRADATION, ZEARALENONE, DETOXIFICATION, STRAIN, WHEAT, FEED

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MLA
Vanhoutte, Ilse, et al. “Bacterial Enrichment Cultures Biotransform the Mycotoxin Deoxynivalenol into a Novel Metabolite Toxic to Plant and Porcine Cells.” TOXINS, vol. 13, no. 8, 2021, doi:10.3390/toxins13080552.
APA
Vanhoutte, I., De Tender, C., Demeyere, K., Abdallah, M. F., Ommeslag, S., Vermeir, P., … De Gelder, L. (2021). Bacterial enrichment cultures biotransform the mycotoxin deoxynivalenol into a novel metabolite toxic to plant and porcine cells. TOXINS, 13(8). https://doi.org/10.3390/toxins13080552
Chicago author-date
Vanhoutte, Ilse, Caroline De Tender, Kristel Demeyere, Mohamed Fathi Abdallah, Sarah Ommeslag, Pieter Vermeir, Sarah De Saeger, et al. 2021. “Bacterial Enrichment Cultures Biotransform the Mycotoxin Deoxynivalenol into a Novel Metabolite Toxic to Plant and Porcine Cells.” TOXINS 13 (8). https://doi.org/10.3390/toxins13080552.
Chicago author-date (all authors)
Vanhoutte, Ilse, Caroline De Tender, Kristel Demeyere, Mohamed Fathi Abdallah, Sarah Ommeslag, Pieter Vermeir, Sarah De Saeger, Jane Debode, Evelyne Meyer, Siska Croubels, Kris Audenaert, and Leen De Gelder. 2021. “Bacterial Enrichment Cultures Biotransform the Mycotoxin Deoxynivalenol into a Novel Metabolite Toxic to Plant and Porcine Cells.” TOXINS 13 (8). doi:10.3390/toxins13080552.
Vancouver
1.
Vanhoutte I, De Tender C, Demeyere K, Abdallah MF, Ommeslag S, Vermeir P, et al. Bacterial enrichment cultures biotransform the mycotoxin deoxynivalenol into a novel metabolite toxic to plant and porcine cells. TOXINS. 2021;13(8).
IEEE
[1]
I. Vanhoutte et al., “Bacterial enrichment cultures biotransform the mycotoxin deoxynivalenol into a novel metabolite toxic to plant and porcine cells,” TOXINS, vol. 13, no. 8, 2021.
@article{8717272,
  abstract     = {{The mycotoxin deoxynivalenol (DON), produced in wheat, barley and maize by Fusarium graminearum and Fusarium culmorum, is threatening the health of humans and animals. With its worldwide high incidence in food and feed, mitigation strategies are needed to detoxify DON, maintaining the nutritional value and palatability of decontaminated commodities. A promising technique is biological degradation, where microorganisms are used to biotransform mycotoxins into less toxic metabolites. In this study, bacterial enrichment cultures were screened for their DON detoxification potential, where DON and its potential derivatives were monitored. The residual phytotoxicity was determined through a bioassay using the aquatic plant Lemna minor L. Two bacterial enrichment cultures were found to biotransform DON into a still highly toxic metabolite for plants. Furthermore, a cytotoxic effect was observed on the cellular viability of intestinal porcine epithelial cells. Through liquid chromatography high-resolution mass spectrometry analysis, an unknown compound was detected, and tentatively characterized with a molecular weight of 30.0 Da (i.e., CH2O) higher than DON. Metabarcoding of the subsequently enriched bacterial communities revealed a shift towards the genera Sphingopyxis, Pseudoxanthomonas, Ochrobactrum and Pseudarthrobacter. This work describes the discovery of a novel bacterial DON-derived metabolite, toxic to plant and porcine cells.}},
  articleno    = {{552}},
  author       = {{Vanhoutte, Ilse and De Tender, Caroline and Demeyere, Kristel and Abdallah, Mohamed Fathi and Ommeslag, Sarah and Vermeir, Pieter and De Saeger, Sarah and Debode, Jane and Meyer, Evelyne and Croubels, Siska and Audenaert, Kris and De Gelder, Leen}},
  issn         = {{2072-6651}},
  journal      = {{TOXINS}},
  keywords     = {{Toxicology,Health,Toxicology and Mutagenesis,deoxynivalenol derivatives,enrichment,biotransformation,bioassay,cytotoxicity assay,metabarcoding,POLYCYCLIC AROMATIC-HYDROCARBONS,IN-VITRO,TRICHOTHECENE MYCOTOXINS,MICROBIAL-DEGRADATION,BIODEGRADATION,ZEARALENONE,DETOXIFICATION,STRAIN,WHEAT,FEED}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{20}},
  title        = {{Bacterial enrichment cultures biotransform the mycotoxin deoxynivalenol into a novel metabolite toxic to plant and porcine cells}},
  url          = {{http://dx.doi.org/10.3390/toxins13080552}},
  volume       = {{13}},
  year         = {{2021}},
}
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