Advanced search
1 file | 3.55 MB Add to list

Nature and nurture : genotype-dependent differential responses of root architecture to agar and soil environments

(2021) GENES. 12(7).
Author
Organization
Abstract
Root development is crucial for plant growth and therefore a key factor in plant performance and food production. Arabidopsis thaliana is the most commonly used system to study root system architecture (RSA). Growing plants on agar-based media has always been routine practice, but this approach poorly reflects the natural situation, which fact in recent years has led to a dramatic shift toward studying RSA in soil. Here, we directly compare RSA responses to agar-based medium (plates) and potting soil (rhizotrons) for a set of redundant loss-of-function plethora (plt) CRISPR mutants with variable degrees of secondary root defects. We demonstrate that plt3plt7 and plt3plt5plt7 plants, which produce only a handful of emerged secondary roots, can be distinguished from other genotypes based on both RSA shape and individual traits on plates and rhizotrons. However, in rhizotrons the secondary root density and the total contribution of the side root system to the RSA is increased in these two mutants, effectively rendering their phenotypes less distinct compared to WT. On the other hand, plt3, plt3plt5, and plt5plt7 mutants showed an opposite effect by having reduced secondary root density in rhizotrons. This leads us to believe that plate versus rhizotron responses are genotype dependent, and these differential responses were also observed in unrelated mutants short-root and scarecrow. Our study demonstrates that the type of growth system affects the RSA differently across genotypes, hence the optimal choice of growth conditions to analyze RSA phenotype is not predetermined.
Keywords
Genetics(clinical), Genetics, root system architecture, Arabidopsis thaliana, rhizotron, PLETHORA, geometric morphometrics, ARABIDOPSIS ROOT, RADIAL ORGANIZATION, SYSTEM, SCARECROW

Downloads

  • Kerstens et al. 2021 Genes 12 1028.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 3.55 MB

Citation

Please use this url to cite or link to this publication:

MLA
Kerstens, Merijn, et al. “Nature and Nurture : Genotype-Dependent Differential Responses of Root Architecture to Agar and Soil Environments.” GENES, vol. 12, no. 7, 2021, doi:10.3390/genes12071028.
APA
Kerstens, M., Hesen, V., Yalamanchili, K., Bimbo, A., Grigg, S., Opdenacker, D., … Willemsen, V. (2021). Nature and nurture : genotype-dependent differential responses of root architecture to agar and soil environments. GENES, 12(7). https://doi.org/10.3390/genes12071028
Chicago author-date
Kerstens, Merijn, Vera Hesen, Kavya Yalamanchili, Andrea Bimbo, Stephen Grigg, Davy Opdenacker, Tom Beeckman, Renze Heidstra, and Viola Willemsen. 2021. “Nature and Nurture : Genotype-Dependent Differential Responses of Root Architecture to Agar and Soil Environments.” GENES 12 (7). https://doi.org/10.3390/genes12071028.
Chicago author-date (all authors)
Kerstens, Merijn, Vera Hesen, Kavya Yalamanchili, Andrea Bimbo, Stephen Grigg, Davy Opdenacker, Tom Beeckman, Renze Heidstra, and Viola Willemsen. 2021. “Nature and Nurture : Genotype-Dependent Differential Responses of Root Architecture to Agar and Soil Environments.” GENES 12 (7). doi:10.3390/genes12071028.
Vancouver
1.
Kerstens M, Hesen V, Yalamanchili K, Bimbo A, Grigg S, Opdenacker D, et al. Nature and nurture : genotype-dependent differential responses of root architecture to agar and soil environments. GENES. 2021;12(7).
IEEE
[1]
M. Kerstens et al., “Nature and nurture : genotype-dependent differential responses of root architecture to agar and soil environments,” GENES, vol. 12, no. 7, 2021.
@article{8717231,
  abstract     = {{Root development is crucial for plant growth and therefore a key factor in plant performance and food production. Arabidopsis thaliana is the most commonly used system to study root system architecture (RSA). Growing plants on agar-based media has always been routine practice, but this approach poorly reflects the natural situation, which fact in recent years has led to a dramatic shift toward studying RSA in soil. Here, we directly compare RSA responses to agar-based medium (plates) and potting soil (rhizotrons) for a set of redundant loss-of-function plethora (plt) CRISPR mutants with variable degrees of secondary root defects. We demonstrate that plt3plt7 and plt3plt5plt7 plants, which produce only a handful of emerged secondary roots, can be distinguished from other genotypes based on both RSA shape and individual traits on plates and rhizotrons. However, in rhizotrons the secondary root density and the total contribution of the side root system to the RSA is increased in these two mutants, effectively rendering their phenotypes less distinct compared to WT. On the other hand, plt3, plt3plt5, and plt5plt7 mutants showed an opposite effect by having reduced secondary root density in rhizotrons. This leads us to believe that plate versus rhizotron responses are genotype dependent, and these differential responses were also observed in unrelated mutants short-root and scarecrow. Our study demonstrates that the type of growth system affects the RSA differently across genotypes, hence the optimal choice of growth conditions to analyze RSA phenotype is not predetermined.}},
  articleno    = {{1028}},
  author       = {{Kerstens, Merijn and Hesen, Vera and Yalamanchili, Kavya and Bimbo, Andrea and Grigg, Stephen and Opdenacker, Davy and Beeckman, Tom and Heidstra, Renze and Willemsen, Viola}},
  issn         = {{2073-4425}},
  journal      = {{GENES}},
  keywords     = {{Genetics(clinical),Genetics,root system architecture,Arabidopsis thaliana,rhizotron,PLETHORA,geometric morphometrics,ARABIDOPSIS ROOT,RADIAL ORGANIZATION,SYSTEM,SCARECROW}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{14}},
  title        = {{Nature and nurture : genotype-dependent differential responses of root architecture to agar and soil environments}},
  url          = {{http://dx.doi.org/10.3390/genes12071028}},
  volume       = {{12}},
  year         = {{2021}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: