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Photoporation with biodegradable polydopamine nanosensitizers enables safe and efficient delivery of mRNA in human T cells

Aranit Harizaj (UGent) , Mike Wels, Laurens Raes, Stephan Stremersch (UGent) , Glenn Goetgeluk (UGent) , Toon Brans (UGent) , Bart Vandekerckhove (UGent) , Félix Sauvage (UGent) , Stefaan De Smedt (UGent) , Ine Lentacker (UGent) , et al.
(2021) ADVANCED FUNCTIONAL MATERIALS. 31(28).
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Abstract
Safe and efficient production of chimeric antigen receptor (CAR)-T cells is of crucial importance for cell-based cancer immunotherapy. Physical transfection methods have quickly gained in importance to transfect T-cells, being readily compatible with different cell types and a broad variety of cargo molecules. In particular, nanoparticle-sensitized photoporation has been introduced in recent years as a gentle yet efficient method to transiently permeabilize cells, allowing subsequent entry of external cargo molecules into the cells. Gold nanoparticles (AuNPs) have been used the most as photothermal sensitizers because they can easily form laser-induced vapor nanobubbles, a photothermal phenomenon that is shown to be particularly efficient for permeabilizing cells. However, as AuNPs are not biodegradable, clinical translation is hampered. Here, for the first time, it is reported on the possibility to form laser-induced vapor nanobubbles from biocompatible polymeric nanoparticles. Compared to electroporation as the most used physical transfection method for T cells, 2.5 times more living mRNA transfected human T cells are obtained via photoporation sensitized by polydopamine nanoparticles. Together, it shows that photoporation is a viable approach for efficiently producing therapeutic engineered T-cells at a throughput easily exceeding 10(5) cells per second.
Keywords
cancer immunotherapycell therapydrug deliveryhuman T cellsmessenger RNAphotoporationphotothermal nanoparticlespolydopaminetransfection

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Citation

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MLA
Harizaj, Aranit, et al. “Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of MRNA in Human T Cells.” ADVANCED FUNCTIONAL MATERIALS, vol. 31, no. 28, 2021, doi:10.1002/adfm.202102472.
APA
Harizaj, A., Wels, M., Raes, L., Stremersch, S., Goetgeluk, G., Brans, T., … Braeckmans, K. (2021). Photoporation with biodegradable polydopamine nanosensitizers enables safe and efficient delivery of mRNA in human T cells. ADVANCED FUNCTIONAL MATERIALS, 31(28). https://doi.org/10.1002/adfm.202102472
Chicago author-date
Harizaj, Aranit, Mike Wels, Laurens Raes, Stephan Stremersch, Glenn Goetgeluk, Toon Brans, Bart Vandekerckhove, et al. 2021. “Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of MRNA in Human T Cells.” ADVANCED FUNCTIONAL MATERIALS 31 (28). https://doi.org/10.1002/adfm.202102472.
Chicago author-date (all authors)
Harizaj, Aranit, Mike Wels, Laurens Raes, Stephan Stremersch, Glenn Goetgeluk, Toon Brans, Bart Vandekerckhove, Félix Sauvage, Stefaan De Smedt, Ine Lentacker, and Kevin Braeckmans. 2021. “Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of MRNA in Human T Cells.” ADVANCED FUNCTIONAL MATERIALS 31 (28). doi:10.1002/adfm.202102472.
Vancouver
1.
Harizaj A, Wels M, Raes L, Stremersch S, Goetgeluk G, Brans T, et al. Photoporation with biodegradable polydopamine nanosensitizers enables safe and efficient delivery of mRNA in human T cells. ADVANCED FUNCTIONAL MATERIALS. 2021;31(28).
IEEE
[1]
A. Harizaj et al., “Photoporation with biodegradable polydopamine nanosensitizers enables safe and efficient delivery of mRNA in human T cells,” ADVANCED FUNCTIONAL MATERIALS, vol. 31, no. 28, 2021.
@article{8717064,
  abstract     = {{Safe and efficient production of chimeric antigen receptor (CAR)-T cells is of crucial importance for cell-based cancer immunotherapy. Physical transfection methods have quickly gained in importance to transfect T-cells, being readily compatible with different cell types and a broad variety of cargo molecules. In particular, nanoparticle-sensitized photoporation has been introduced in recent years as a gentle yet efficient method to transiently permeabilize cells, allowing subsequent entry of external cargo molecules into the cells. Gold nanoparticles (AuNPs) have been used the most as photothermal sensitizers because they can easily form laser-induced vapor nanobubbles, a photothermal phenomenon that is shown to be particularly efficient for permeabilizing cells. However, as AuNPs are not biodegradable, clinical translation is hampered. Here, for the first time, it is reported on the possibility to form laser-induced vapor nanobubbles from biocompatible polymeric nanoparticles. Compared to electroporation as the most used physical transfection method for T cells, 2.5 times more living mRNA transfected human T cells are obtained via photoporation sensitized by polydopamine nanoparticles. Together, it shows that photoporation is a viable approach for efficiently producing therapeutic engineered T-cells at a throughput easily exceeding 10(5) cells per second.}},
  articleno    = {{2102472}},
  author       = {{Harizaj, Aranit and Wels, Mike and Raes, Laurens and Stremersch, Stephan and Goetgeluk, Glenn and Brans, Toon and Vandekerckhove, Bart and Sauvage, Félix and De Smedt, Stefaan and Lentacker, Ine and Braeckmans, Kevin}},
  issn         = {{1616-301X}},
  journal      = {{ADVANCED FUNCTIONAL MATERIALS}},
  keywords     = {{cancer immunotherapycell therapydrug deliveryhuman T cellsmessenger RNAphotoporationphotothermal nanoparticlespolydopaminetransfection}},
  language     = {{eng}},
  number       = {{28}},
  pages        = {{12}},
  title        = {{Photoporation with biodegradable polydopamine nanosensitizers enables safe and efficient delivery of mRNA in human T cells}},
  url          = {{http://doi.org/10.1002/adfm.202102472}},
  volume       = {{31}},
  year         = {{2021}},
}
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