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PapRIV : a BV-2 microglial cell activating quorum sensing peptide

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Abstract
Quorum sensing peptides (QSPs) are bacterial peptides produced by Gram-positive bacteria to communicate with their peers in a cell-density dependent manner. These peptides do not only act as interbacterial communication signals, but can also have effects on the host. Compelling evidence demonstrates the presence of a gut-brain axis and more specifically, the role of the gut microbiota in microglial functioning. The aim of this study is to investigate microglial activating properties of a selected QSP (PapRIV) which is produced by <jats:italic>Bacillus cereus</jats:italic> species. PapRIV showed in vitro activating properties of BV-2 microglia cells and was able to cross the in vitro Caco-2 cell model and reach the brain. In vivo peptide presence was also demonstrated in mouse plasma. The peptide caused induction of IL-6, TNFα and ROS expression and increased the fraction of ameboid BV-2 microglia cells in an NF-κB dependent manner. Different metabolites were identified in serum, of which the main metabolite still remained active. PapRIV is thus able to cross the gastro-intestinal tract and the blood–brain barrier and shows in vitro activating properties in BV-2 microglia cells, hereby indicating a potential role of this quorum sensing peptide in gut-brain interaction.
Keywords
QSP, microglia, neurodegenaration, neuro-inflammation

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MLA
Janssens, Yorick, et al. “PapRIV : A BV-2 Microglial Cell Activating Quorum Sensing Peptide.” SCIENTIFIC REPORTS, vol. 11, no. 1, 2021, doi:10.1038/s41598-021-90030-y.
APA
Janssens, Y., Debunne, N., De Spiegeleer, A., Wynendaele, E., Planas, M., Feliu, L., … De Spiegeleer, B. (2021). PapRIV : a BV-2 microglial cell activating quorum sensing peptide. SCIENTIFIC REPORTS, 11(1). https://doi.org/10.1038/s41598-021-90030-y
Chicago author-date
Janssens, Yorick, Nathan Debunne, Anton De Spiegeleer, Evelien Wynendaele, Marta Planas, Lidia Feliu, Alessandra Quarta, et al. 2021. “PapRIV : A BV-2 Microglial Cell Activating Quorum Sensing Peptide.” SCIENTIFIC REPORTS 11 (1). https://doi.org/10.1038/s41598-021-90030-y.
Chicago author-date (all authors)
Janssens, Yorick, Nathan Debunne, Anton De Spiegeleer, Evelien Wynendaele, Marta Planas, Lidia Feliu, Alessandra Quarta, Christel Claes, Debby Van Dam, Peter Paul De Deyn, Peter Ponsaerts, Matthew Blurton-Jones, and Bart De Spiegeleer. 2021. “PapRIV : A BV-2 Microglial Cell Activating Quorum Sensing Peptide.” SCIENTIFIC REPORTS 11 (1). doi:10.1038/s41598-021-90030-y.
Vancouver
1.
Janssens Y, Debunne N, De Spiegeleer A, Wynendaele E, Planas M, Feliu L, et al. PapRIV : a BV-2 microglial cell activating quorum sensing peptide. SCIENTIFIC REPORTS. 2021;11(1).
IEEE
[1]
Y. Janssens et al., “PapRIV : a BV-2 microglial cell activating quorum sensing peptide,” SCIENTIFIC REPORTS, vol. 11, no. 1, 2021.
@article{8716644,
  abstract     = {{Quorum sensing peptides (QSPs) are bacterial peptides produced by Gram-positive bacteria to communicate with their peers in a cell-density dependent manner. These peptides do not only act as interbacterial communication signals, but can also have effects on the host. Compelling evidence demonstrates the presence of a gut-brain axis and more specifically, the role of the gut microbiota in microglial functioning. The aim of this study is to investigate microglial activating properties of a selected QSP (PapRIV) which is produced by <jats:italic>Bacillus cereus</jats:italic> species. PapRIV showed in vitro activating properties of BV-2 microglia cells and was able to cross the in vitro Caco-2 cell model and reach the brain. In vivo peptide presence was also demonstrated in mouse plasma. The peptide caused induction of IL-6, TNFα and ROS expression and increased the fraction of ameboid BV-2 microglia cells in an NF-κB dependent manner. Different metabolites were identified in serum, of which the main metabolite still remained active. PapRIV is thus able to cross the gastro-intestinal tract and the blood–brain barrier and shows in vitro activating properties in BV-2 microglia cells, hereby indicating a potential role of this quorum sensing peptide in gut-brain interaction.}},
  articleno    = {{10723}},
  author       = {{Janssens, Yorick and Debunne, Nathan and De Spiegeleer, Anton and Wynendaele, Evelien and Planas, Marta and Feliu, Lidia and Quarta, Alessandra and Claes, Christel and Van Dam, Debby and De Deyn, Peter Paul and Ponsaerts, Peter and Blurton-Jones, Matthew and De Spiegeleer, Bart}},
  issn         = {{2045-2322}},
  journal      = {{SCIENTIFIC REPORTS}},
  keywords     = {{QSP,microglia,neurodegenaration,neuro-inflammation}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{14}},
  title        = {{PapRIV : a BV-2 microglial cell activating quorum sensing peptide}},
  url          = {{http://dx.doi.org/10.1038/s41598-021-90030-y}},
  volume       = {{11}},
  year         = {{2021}},
}

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