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Propagation of spontaneous electrical activity in the ex vivo human uterus

Nienke P. M. Kuijsters, Federica Sammali, Xin Ye, Celine Blank (UGent) , Lin Xu, Massimo Mischi, Benedictus C. Schoot (UGent) and Chiara Rabotti
(2020) PFLUGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY. 472(8). p.1065-1078
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Abstract
Contractions of the non-pregnant uterus play a key role in fertility. Yet, the electrophysiology underlying these contractions is poorly understood. In this paper, we investigate the presence of uterine electrical activity and characterize its propagation in unstimulated ex vivo human uteri. Multichannel electrohysterographic measurements were performed in five freshly resected human uteri starting immediately after hysterectomy. Using an electrode grid externally and an electrode array internally, measurements were performed up to 24 h after hysterectomy and compared with control. Up to 2 h after hysterectomy, we measured biopotentials in all included uteri. The median root mean squared (RMS) values of the external measurements ranged between 3.95 mu V (interquartile range (IQR) 2.41-14.18 mu V) and 39.4 mu V (interquartile range (IQR) 10.84-105.64 mu V) and were all significantly higher than control (median RMS of 1.69 mu V, IQR 1.13-3.11 mu V), consisting of chicken breast meat. The RMS values decreased significantly over time. After 24 h, the median RMS (1.27 mu V, IQR 0.86-3.04 mu V) was comparable with the control (1.69 mu V, IQR 1.13-3.11 mu V,p = 0.125). The internal measurements showed a comparable pattern over time, but overall lower amplitude. The measured biopotentials propagated over the uterine surface, following both a plane-wave as well as an erratic pattern. No clear pacemaker location nor a preferred propagation direction could be identified. These results show that ex vivo uteri can spontaneously generate propagating biopotentials and provide novel insight contributing to improving our understanding of the electrophysiology of the human non-pregnant uterus.
Keywords
UTERINE PERISTALSIS, EXTRACORPOREAL PERFUSION, INTERSTITIAL-CELLS, EXPERIMENTAL-MODEL, PRETERM DELIVERY, SPERM TRANSPORT, PREGNANCY, CONTRACTILITY, ENDOMETRIOSIS, ELECTROHYSTEROGRAM, Uterine electrophysiology, Uterine contractility, Smooth muscle, Propagation, Pacemaker, Fertility

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MLA
Kuijsters, Nienke P. M., et al. “Propagation of Spontaneous Electrical Activity in the Ex Vivo Human Uterus.” PFLUGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY, vol. 472, no. 8, 2020, pp. 1065–78, doi:10.1007/s00424-020-02426-w.
APA
Kuijsters, N. P. M., Sammali, F., Ye, X., Blank, C., Xu, L., Mischi, M., … Rabotti, C. (2020). Propagation of spontaneous electrical activity in the ex vivo human uterus. PFLUGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY, 472(8), 1065–1078. https://doi.org/10.1007/s00424-020-02426-w
Chicago author-date
Kuijsters, Nienke P. M., Federica Sammali, Xin Ye, Celine Blank, Lin Xu, Massimo Mischi, Benedictus C. Schoot, and Chiara Rabotti. 2020. “Propagation of Spontaneous Electrical Activity in the Ex Vivo Human Uterus.” PFLUGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY 472 (8): 1065–78. https://doi.org/10.1007/s00424-020-02426-w.
Chicago author-date (all authors)
Kuijsters, Nienke P. M., Federica Sammali, Xin Ye, Celine Blank, Lin Xu, Massimo Mischi, Benedictus C. Schoot, and Chiara Rabotti. 2020. “Propagation of Spontaneous Electrical Activity in the Ex Vivo Human Uterus.” PFLUGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY 472 (8): 1065–1078. doi:10.1007/s00424-020-02426-w.
Vancouver
1.
Kuijsters NPM, Sammali F, Ye X, Blank C, Xu L, Mischi M, et al. Propagation of spontaneous electrical activity in the ex vivo human uterus. PFLUGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY. 2020;472(8):1065–78.
IEEE
[1]
N. P. M. Kuijsters et al., “Propagation of spontaneous electrical activity in the ex vivo human uterus,” PFLUGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY, vol. 472, no. 8, pp. 1065–1078, 2020.
@article{8715687,
  abstract     = {{Contractions of the non-pregnant uterus play a key role in fertility. Yet, the electrophysiology underlying these contractions is poorly understood. In this paper, we investigate the presence of uterine electrical activity and characterize its propagation in unstimulated ex vivo human uteri. Multichannel electrohysterographic measurements were performed in five freshly resected human uteri starting immediately after hysterectomy. Using an electrode grid externally and an electrode array internally, measurements were performed up to 24 h after hysterectomy and compared with control. Up to 2 h after hysterectomy, we measured biopotentials in all included uteri. The median root mean squared (RMS) values of the external measurements ranged between 3.95 mu V (interquartile range (IQR) 2.41-14.18 mu V) and 39.4 mu V (interquartile range (IQR) 10.84-105.64 mu V) and were all significantly higher than control (median RMS of 1.69 mu V, IQR 1.13-3.11 mu V), consisting of chicken breast meat. The RMS values decreased significantly over time. After 24 h, the median RMS (1.27 mu V, IQR 0.86-3.04 mu V) was comparable with the control (1.69 mu V, IQR 1.13-3.11 mu V,p = 0.125). The internal measurements showed a comparable pattern over time, but overall lower amplitude. The measured biopotentials propagated over the uterine surface, following both a plane-wave as well as an erratic pattern. No clear pacemaker location nor a preferred propagation direction could be identified. These results show that ex vivo uteri can spontaneously generate propagating biopotentials and provide novel insight contributing to improving our understanding of the electrophysiology of the human non-pregnant uterus.}},
  author       = {{Kuijsters, Nienke P. M. and Sammali, Federica and Ye, Xin and Blank, Celine and Xu, Lin and Mischi, Massimo and Schoot, Benedictus C. and Rabotti, Chiara}},
  issn         = {{0031-6768}},
  journal      = {{PFLUGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY}},
  keywords     = {{UTERINE PERISTALSIS,EXTRACORPOREAL PERFUSION,INTERSTITIAL-CELLS,EXPERIMENTAL-MODEL,PRETERM DELIVERY,SPERM TRANSPORT,PREGNANCY,CONTRACTILITY,ENDOMETRIOSIS,ELECTROHYSTEROGRAM,Uterine electrophysiology,Uterine contractility,Smooth muscle,Propagation,Pacemaker,Fertility}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1065--1078}},
  title        = {{Propagation of spontaneous electrical activity in the ex vivo human uterus}},
  url          = {{http://dx.doi.org/10.1007/s00424-020-02426-w}},
  volume       = {{472}},
  year         = {{2020}},
}
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