@article{8715663,
  abstract     = {{Research question: Ovarian stimulation during IVF cycles involves close monitoring of oestradiol, progesterone and ultrasound measurements of follicle growth. In contrast to blood draws, sampling saliva is less invasive. Here, a blind validation is presented of a novel saliva-based oestradiol and progesterone assay carried out in samples collected in independent IVF clinics. Design: Concurrent serum and saliva samples were collected from 324 patients at six large independent IVF laboratories. Saliva samples were frozen and run blinded. A further 18 patients had samples collected more frequently around the time of HCG trigger. Saliva samples were analysed using an immunoassay developed with Salimetrics LLC. Results: In total, 652 pairs of saliva and serum oestradiol were evaluated, with correlation coefficients ranging from 0.68 to 0.91. In the European clinics, a further 237 of saliva and serum progesterone samples were evaluated; however, the correlations were generally poorer, ranging from -0.02 to 0.22. In the patients collected more frequently, five out of 18 patients (27.8%) showed an immediate decrease in oestradiol after trigger. When progesterone samples were assessed after trigger, eight out of 18 (44.4%) showed a continued rise. Conclusions: Salivary oestradiol hormone testing correlates well to serum-based assessment, whereas progesterone values, around the time of trigger, are not consistent from patient to patient.

ABSTRACT Research question: Ovarian stimulation during IVF cycles involves close monitoring of oestradiol, progesterone and ultrasound measurements of follicle growth. In contrast to blood draws, sampling saliva is less invasive. Here, a blind validation is presented of a novel saliva-based oestradiol and progesterone assay carried out in samples collected in independent IVF clinics. Design: Concurrent serum and saliva samples were collected from 324 patients at six large independent IVF laboratories. Saliva samples were frozen and run blinded. A further 18 patients had samples collected more frequently around the time of HCG trigger. Saliva samples were analysed using an immunoassay developed with Salimetrics LLC. Results: In total, 652 pairs of saliva and serum oestradiol were evaluated, with correlation coefficients ranging from 0.68 to 0.91. In the European clinics, a further 237 of saliva and serum progesterone samples were evaluated; however, the correlations were generally poorer, ranging from -0.02 to 0.22. In the patients collected more frequently, five out of 18 patients (27.8%) showed an immediate decrease in oestradiol after trigger. When progesterone samples were assessed after trigger, eight out of 18 (44.4%) showed a continued rise. Conclusions: Salivary oestradiol hormone testing correlates well to serum-based assessment, whereas progesterone values, around the time of trigger, are not consistent from patient to patient.}},
  author       = {{Sakkas, Denny and Howles, Colin M. and Atkinson, Leslie and Borini, Andrea and Bosch, Ernesto A. and Bryce, Crystal and Cattoli, Monica and Copperman, Alan B. and de Bantel, Astrid Finet and French, Brian and Gerris, Jan and Granger, Steve W. and Grzegorczyk-Martin, Veronika and Lee, Joseph A. and Levy, Michael J. and Matin, Marla J. and Somers, Sara and Widra, Eric A. and Alper, Michael M.}},
  issn         = {{1472-6483}},
  journal      = {{REPRODUCTIVE BIOMEDICINE ONLINE}},
  keywords     = {{Luteal support,Ovarian stimulation monitoring,Saliva estradiol,Saliva,progesterone}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{421--428}},
  title        = {{A multi-centre international study of salivary hormone oestradiol and progesterone measurements in ART monitoring}},
  url          = {{http://doi.org/10.1016/j.rbmo.2020.10.012}},
  volume       = {{42}},
  year         = {{2021}},
}

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