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Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro

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Abstract
Communications via quorum sensing (QS) between non-aureus staphylococci (NAS) and Staphylococcus (S.) aureus in the bovine mammary gland remains largely unexplored. We determined whether 34 S. chromogenes, 11 S. epidermidis, and 14 S. simulans isolates originating from bovine milk samples and teat apices were able to regulate the QS of S. aureus, and if so, how in vitro growth inhibition of S. aureus by NAS, or NAS metabolites, or NAS cells themselves play a role in this process. In co-culture with S. aureus we observed that these 3 NAS species in general downregulated the expression of rnaIII, the effector molecule of the QS system, but this effect was more pronounced in S. chromogenes and S. simulans isolates than in S. epidermidis isolates. In vitro growth inhibition of S. aureus by NAS resulted in a small underestimation of the downregulating effect of NAS on rnaIII expression of S. aureus. Additionally, the culture supernatant of these NAS isolates and supernatant treated with proteinase K expressed greater regulatory activity over S. aureus virulence genes rnaIII, hla, and spa than washed NAS cells suspended in sterile water. These microbial interactions may influence S. aureus virulence and pathogenesis within the host. Isolation and identification of NAS metabolites affecting the QS system of S. aureus might help to develop alternative strategies for treatment and control of S. aureus mastitis.
Keywords
COAGULASE-NEGATIVE STAPHYLOCOCCI, SOMATIC-CELL COUNT, INTRAMAMMARY, INFECTION, MASTITIS PATHOGENS, BIOFILM FORMATION, DAIRY-COWS, MILK-YIELD, INHIBITION, COLONIZATION, STRAINS, Coagulase-negative staphylococci, Staphylococcus aureus, agr, Quorum, sensing, Mastitis

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MLA
Toledo-Silva, Bruno, et al. “Metabolites of Bovine-Associated Non-Aureus Staphylococci Influence Expression of Staphylococcus Aureus Agr-Related Genes in Vitro.” VETERINARY RESEARCH, vol. 52, no. 1, 2021, doi:10.1186/s13567-021-00933-x.
APA
Toledo-Silva, B., Nogueira De Souza, F., Piepers, S., Mertens, K., Haesebrouck, F., & De Vliegher, S. (2021). Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro. VETERINARY RESEARCH, 52(1). https://doi.org/10.1186/s13567-021-00933-x
Chicago author-date
Toledo-Silva, Bruno, Fernando Nogueira De Souza, Sofie Piepers, Kristien Mertens, Freddy Haesebrouck, and Sarne De Vliegher. 2021. “Metabolites of Bovine-Associated Non-Aureus Staphylococci Influence Expression of Staphylococcus Aureus Agr-Related Genes in Vitro.” VETERINARY RESEARCH 52 (1). https://doi.org/10.1186/s13567-021-00933-x.
Chicago author-date (all authors)
Toledo-Silva, Bruno, Fernando Nogueira De Souza, Sofie Piepers, Kristien Mertens, Freddy Haesebrouck, and Sarne De Vliegher. 2021. “Metabolites of Bovine-Associated Non-Aureus Staphylococci Influence Expression of Staphylococcus Aureus Agr-Related Genes in Vitro.” VETERINARY RESEARCH 52 (1). doi:10.1186/s13567-021-00933-x.
Vancouver
1.
Toledo-Silva B, Nogueira De Souza F, Piepers S, Mertens K, Haesebrouck F, De Vliegher S. Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro. VETERINARY RESEARCH. 2021;52(1).
IEEE
[1]
B. Toledo-Silva, F. Nogueira De Souza, S. Piepers, K. Mertens, F. Haesebrouck, and S. De Vliegher, “Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro,” VETERINARY RESEARCH, vol. 52, no. 1, 2021.
@article{8714597,
  abstract     = {{Communications via quorum sensing (QS) between non-aureus staphylococci (NAS) and Staphylococcus (S.) aureus in the bovine mammary gland remains largely unexplored. We determined whether 34 S. chromogenes, 11 S. epidermidis, and 14 S. simulans isolates originating from bovine milk samples and teat apices were able to regulate the QS of S. aureus, and if so, how in vitro growth inhibition of S. aureus by NAS, or NAS metabolites, or NAS cells themselves play a role in this process. In co-culture with S. aureus we observed that these 3 NAS species in general downregulated the expression of rnaIII, the effector molecule of the QS system, but this effect was more pronounced in S. chromogenes and S. simulans isolates than in S. epidermidis isolates. In vitro growth inhibition of S. aureus by NAS resulted in a small underestimation of the downregulating effect of NAS on rnaIII expression of S. aureus. Additionally, the culture supernatant of these NAS isolates and supernatant treated with proteinase K expressed greater regulatory activity over S. aureus virulence genes rnaIII, hla, and spa than washed NAS cells suspended in sterile water. These microbial interactions may influence S. aureus virulence and pathogenesis within the host. Isolation and identification of NAS metabolites affecting the QS system of S. aureus might help to develop alternative strategies for treatment and control of S. aureus mastitis.}},
  articleno    = {{62}},
  author       = {{Toledo-Silva, Bruno and Nogueira De Souza, Fernando and Piepers, Sofie and Mertens, Kristien and Haesebrouck, Freddy and De Vliegher, Sarne}},
  issn         = {{0928-4249}},
  journal      = {{VETERINARY RESEARCH}},
  keywords     = {{COAGULASE-NEGATIVE STAPHYLOCOCCI,SOMATIC-CELL COUNT,INTRAMAMMARY,INFECTION,MASTITIS PATHOGENS,BIOFILM FORMATION,DAIRY-COWS,MILK-YIELD,INHIBITION,COLONIZATION,STRAINS,Coagulase-negative staphylococci,Staphylococcus aureus,agr,Quorum,sensing,Mastitis}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{14}},
  title        = {{Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro}},
  url          = {{http://dx.doi.org/10.1186/s13567-021-00933-x}},
  volume       = {{52}},
  year         = {{2021}},
}

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