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RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens

Qun Yang (UGent) , Kristof De Schutter (UGent) , Pengyu Chen (UGent) , Els Van Damme (UGent) and Guy Smagghe (UGent)
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Abstract
Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N-glycosylation in the development of insects, a functional characterization of the N-glycosylation-related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi-mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N-glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the alpha-1,6-fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene-silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.
Keywords
development, glycosylation, Nilaparvata lugens, N&#8208, glycan, postembryonic, RNA interference

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MLA
Yang, Qun, et al. “RNAi of the N-Glycosylation-Related Genes Confirms Their Importance in Insect Development and Alpha-1,6-Fucosyltransferase Plays a Role in the Ecdysis Event for the Hemimetabolous Pest Insect Nilaparvata Lugens.” INSECT SCIENCE, 2021, doi:10.1111/1744-7917.12920.
APA
Yang, Q., De Schutter, K., Chen, P., Van Damme, E., & Smagghe, G. (2021). RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens. INSECT SCIENCE. https://doi.org/10.1111/1744-7917.12920
Chicago author-date
Yang, Qun, Kristof De Schutter, Pengyu Chen, Els Van Damme, and Guy Smagghe. 2021. “RNAi of the N-Glycosylation-Related Genes Confirms Their Importance in Insect Development and Alpha-1,6-Fucosyltransferase Plays a Role in the Ecdysis Event for the Hemimetabolous Pest Insect Nilaparvata Lugens.” INSECT SCIENCE. https://doi.org/10.1111/1744-7917.12920.
Chicago author-date (all authors)
Yang, Qun, Kristof De Schutter, Pengyu Chen, Els Van Damme, and Guy Smagghe. 2021. “RNAi of the N-Glycosylation-Related Genes Confirms Their Importance in Insect Development and Alpha-1,6-Fucosyltransferase Plays a Role in the Ecdysis Event for the Hemimetabolous Pest Insect Nilaparvata Lugens.” INSECT SCIENCE. doi:10.1111/1744-7917.12920.
Vancouver
1.
Yang Q, De Schutter K, Chen P, Van Damme E, Smagghe G. RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens. INSECT SCIENCE. 2021;
IEEE
[1]
Q. Yang, K. De Schutter, P. Chen, E. Van Damme, and G. Smagghe, “RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens,” INSECT SCIENCE, 2021.
@article{8714146,
  abstract     = {{Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N-glycosylation in the development of insects, a functional characterization of the N-glycosylation-related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi-mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N-glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the alpha-1,6-fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene-silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.}},
  author       = {{Yang, Qun and De Schutter, Kristof and Chen, Pengyu and Van Damme, Els and Smagghe, Guy}},
  issn         = {{1672-9609}},
  journal      = {{INSECT SCIENCE}},
  keywords     = {{development,glycosylation,Nilaparvata lugens,N&#8208,glycan,postembryonic,RNA interference}},
  language     = {{eng}},
  title        = {{RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens}},
  url          = {{http://dx.doi.org/10.1111/1744-7917.12920}},
  year         = {{2021}},
}

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