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Operational characteristics of an antibody detecting point of care test for Taenia solium infections in a community and hospital setting

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Abstract
Background: Diagnostic test evaluation includes measures of performance and assessment of operational characteristics. The latter focuses on end-user understanding of instructions to perform the test, ease of use, test turnaround time and ease of result interpretation. This study aimed to assess user comprehension of training for and ease of use of a Taenia solium point of care test (TS POC) evaluated in a community and hospital setting in Zambia and Tanzania, respectively. Methods: The TS POC is a three-step in-house-produced rapid diagnostic test (RDT) for the simultaneous detection of taeniosis (TST) and cysticercosis (TSCC) antibodies. Data collected by administering questionnaires to 29 end-users and from the main evaluation database was analyzed quantitatively. Results: End-users (28/29, 97%) perceived that the training they received for performing the test was sufficient. They performed 4080 tests, of which 80 were invalid. The community-based study and TST tests had higher invalid rates. The overall result interpretation was within the acceptable range of RDTs with an overall disagreement between readers of 3.3%. The Kappa coefficient of agreement was 85 and 82% for TSCC and TST, respectively. There was more disagreement among readers in the community-based study. Conclusion: End-users rated the TS POC kit moderate in terms of ease of use citing long test turnaround time and difficulties in using the blood transfer device. Overall, the operational performance of the TS POC kit and end-users was within the established acceptable performance range.
Keywords
Infectious Diseases, RAPID DIAGNOSTIC-TESTS, HEALTH WORKERS, PERFORMANCE, CLONING

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MLA
Mubanga, Chishimba, et al. “Operational Characteristics of an Antibody Detecting Point of Care Test for Taenia Solium Infections in a Community and Hospital Setting.” BMC INFECTIOUS DISEASES, vol. 21, no. 1, 2021, doi:10.1186/s12879-021-06320-3.
APA
Mubanga, C., Mwape, K. E., Phiri, I. K., Trevisan, C., Kabululu, M., Zulu, G., … Gabriël, S. (2021). Operational characteristics of an antibody detecting point of care test for Taenia solium infections in a community and hospital setting. BMC INFECTIOUS DISEASES, 21(1). https://doi.org/10.1186/s12879-021-06320-3
Chicago author-date
Mubanga, Chishimba, Kabemba E. Mwape, Isaac K. Phiri, Chiara Trevisan, Mwemezi Kabululu, Gideon Zulu, Inge Van Damme, Veronika Schmidt, Pierre Dorny, and Sarah Gabriël. 2021. “Operational Characteristics of an Antibody Detecting Point of Care Test for Taenia Solium Infections in a Community and Hospital Setting.” BMC INFECTIOUS DISEASES 21 (1). https://doi.org/10.1186/s12879-021-06320-3.
Chicago author-date (all authors)
Mubanga, Chishimba, Kabemba E. Mwape, Isaac K. Phiri, Chiara Trevisan, Mwemezi Kabululu, Gideon Zulu, Inge Van Damme, Veronika Schmidt, Pierre Dorny, and Sarah Gabriël. 2021. “Operational Characteristics of an Antibody Detecting Point of Care Test for Taenia Solium Infections in a Community and Hospital Setting.” BMC INFECTIOUS DISEASES 21 (1). doi:10.1186/s12879-021-06320-3.
Vancouver
1.
Mubanga C, Mwape KE, Phiri IK, Trevisan C, Kabululu M, Zulu G, et al. Operational characteristics of an antibody detecting point of care test for Taenia solium infections in a community and hospital setting. BMC INFECTIOUS DISEASES. 2021;21(1).
IEEE
[1]
C. Mubanga et al., “Operational characteristics of an antibody detecting point of care test for Taenia solium infections in a community and hospital setting,” BMC INFECTIOUS DISEASES, vol. 21, no. 1, 2021.
@article{8714021,
  abstract     = {{Background: Diagnostic test evaluation includes measures of performance and assessment of operational characteristics. The latter focuses on end-user understanding of instructions to perform the test, ease of use, test turnaround time and ease of result interpretation. This study aimed to assess user comprehension of training for and ease of use of a Taenia solium point of care test (TS POC) evaluated in a community and hospital setting in Zambia and Tanzania, respectively.

Methods: The TS POC is a three-step in-house-produced rapid diagnostic test (RDT) for the simultaneous detection of taeniosis (TST) and cysticercosis (TSCC) antibodies. Data collected by administering questionnaires to 29 end-users and from the main evaluation database was analyzed quantitatively.

Results: End-users (28/29, 97%) perceived that the training they received for performing the test was sufficient. They performed 4080 tests, of which 80 were invalid. The community-based study and TST tests had higher invalid rates. The overall result interpretation was within the acceptable range of RDTs with an overall disagreement between readers of 3.3%. The Kappa coefficient of agreement was 85 and 82% for TSCC and TST, respectively. There was more disagreement among readers in the community-based study.

Conclusion: End-users rated the TS POC kit moderate in terms of ease of use citing long test turnaround time and difficulties in using the blood transfer device. Overall, the operational performance of the TS POC kit and end-users was within the established acceptable performance range.}},
  articleno    = {{607}},
  author       = {{Mubanga, Chishimba and Mwape, Kabemba E. and Phiri, Isaac K. and Trevisan, Chiara and Kabululu, Mwemezi and Zulu, Gideon and Van Damme, Inge and Schmidt, Veronika and Dorny, Pierre and Gabriël, Sarah}},
  issn         = {{1471-2334}},
  journal      = {{BMC INFECTIOUS DISEASES}},
  keywords     = {{Infectious Diseases,RAPID DIAGNOSTIC-TESTS,HEALTH WORKERS,PERFORMANCE,CLONING}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{9}},
  title        = {{Operational characteristics of an antibody detecting point of care test for Taenia solium infections in a community and hospital setting}},
  url          = {{http://dx.doi.org/10.1186/s12879-021-06320-3}},
  volume       = {{21}},
  year         = {{2021}},
}

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