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Lateral root initiation and the analysis of gene function using genome editing with CRISPR in Arabidopsis

Nick Vangheluwe (UGent) and Tom Beeckman (UGent)
(2021) GENES. 12(6).
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Abstract
Lateral root initiation is a post-embryonic process that requires the specification of a subset of pericycle cells adjacent to the xylem pole in the primary root into lateral root founder cells. The first visible event of lateral root initiation in Arabidopsis is the simultaneous migration of nuclei in neighbouring founder cells. Coinciding cell cycle activation is essential for founder cells in the pericycle to undergo formative divisions, resulting in the development of a lateral root primordium (LRP). The plant signalling molecule, auxin, is a major regulator of lateral root development; the understanding of the molecular mechanisms controlling lateral root initiation has progressed tremendously by the use of the Arabidopsis model and a continual improvement of molecular methodologies. Here, we provide an overview of the visible events, cell cycle regulators, and auxin signalling cascades related to the initiation of a new LRP. Furthermore, we highlight the potential of genome editing technology to analyse gene function in lateral root initiation, which provides an excellent model to answer fundamental developmental questions such as coordinated cell division, growth axis establishment as well as the specification of cell fate and cell polarity.
Keywords
Genetics(clinical), Genetics, lateral root initiation, asymmetric cell division, auxin signalling, CRISPR, TSKO, genome editing, Arabidopsis, gene function, CELL-CYCLE, AUXIN RESPONSE, F-BOX, DEVELOPMENTAL ANATOMY, DIRECTED MUTAGENESIS, BASAL MERISTEM, PROTEIN, SYSTEM, PERICYCLE, DIVISIONS

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Citation

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MLA
Vangheluwe, Nick, and Tom Beeckman. “Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in Arabidopsis.” GENES, vol. 12, no. 6, 2021, doi:10.3390/genes12060884.
APA
Vangheluwe, N., & Beeckman, T. (2021). Lateral root initiation and the analysis of gene function using genome editing with CRISPR in Arabidopsis. GENES, 12(6). https://doi.org/10.3390/genes12060884
Chicago author-date
Vangheluwe, Nick, and Tom Beeckman. 2021. “Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in Arabidopsis.” GENES 12 (6). https://doi.org/10.3390/genes12060884.
Chicago author-date (all authors)
Vangheluwe, Nick, and Tom Beeckman. 2021. “Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in Arabidopsis.” GENES 12 (6). doi:10.3390/genes12060884.
Vancouver
1.
Vangheluwe N, Beeckman T. Lateral root initiation and the analysis of gene function using genome editing with CRISPR in Arabidopsis. GENES. 2021;12(6).
IEEE
[1]
N. Vangheluwe and T. Beeckman, “Lateral root initiation and the analysis of gene function using genome editing with CRISPR in Arabidopsis,” GENES, vol. 12, no. 6, 2021.
@article{8713756,
  abstract     = {{Lateral root initiation is a post-embryonic process that requires the specification of a subset of pericycle cells adjacent to the xylem pole in the primary root into lateral root founder cells. The first visible event of lateral root initiation in Arabidopsis is the simultaneous migration of nuclei in neighbouring founder cells. Coinciding cell cycle activation is essential for founder cells in the pericycle to undergo formative divisions, resulting in the development of a lateral root primordium (LRP). The plant signalling molecule, auxin, is a major regulator of lateral root development; the understanding of the molecular mechanisms controlling lateral root initiation has progressed tremendously by the use of the Arabidopsis model and a continual improvement of molecular methodologies. Here, we provide an overview of the visible events, cell cycle regulators, and auxin signalling cascades related to the initiation of a new LRP. Furthermore, we highlight the potential of genome editing technology to analyse gene function in lateral root initiation, which provides an excellent model to answer fundamental developmental questions such as coordinated cell division, growth axis establishment as well as the specification of cell fate and cell polarity.}},
  articleno    = {{884}},
  author       = {{Vangheluwe, Nick and Beeckman, Tom}},
  issn         = {{2073-4425}},
  journal      = {{GENES}},
  keywords     = {{Genetics(clinical),Genetics,lateral root initiation,asymmetric cell division,auxin signalling,CRISPR,TSKO,genome editing,Arabidopsis,gene function,CELL-CYCLE,AUXIN RESPONSE,F-BOX,DEVELOPMENTAL ANATOMY,DIRECTED MUTAGENESIS,BASAL MERISTEM,PROTEIN,SYSTEM,PERICYCLE,DIVISIONS}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{14}},
  title        = {{Lateral root initiation and the analysis of gene function using genome editing with CRISPR in Arabidopsis}},
  url          = {{http://dx.doi.org/10.3390/genes12060884}},
  volume       = {{12}},
  year         = {{2021}},
}
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