Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 1.82 MB
Add to list
  1. Search results
  2. CRISPR‐Cas9 editing of Caffeoyl Shiki...

CRISPR‐Cas9 editing of Caffeoyl Shikimate Esterase 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula x P. alba

Lisanne de Vries, Marlies Brouckaert (UGent) , Alexandra Alvarenga Chanoca (UGent) , Hoon Kim, Matthew R. Regner, Vitaliy I. Timokhin, Yi Sun, Barbara De Meester (UGent) , Jan Van Doorsselaere, Geert Goeminne (UGent) , et al.
(2021) PLANT BIOTECHNOLOGY JOURNAL. 19(11). p.2221-2234
Author
Organization
Abstract
Lignins are cell-wall-located aromatic polymers that provide strength and hydrophobicity to woody tissues. Lignin monomers are synthesized via the phenylpropanoid pathway, wherein CAFFEOYL SHIKIMATE ESTERASE (CSE) converts caffeoyl shikimate into caffeic acid. Here, we explored the role of the two CSE homologs in poplar (Populus tremula x P. alba). Reporter lines showed that the expression conferred by both CSE1 and CSE2 promoters is similar. CRISPR-Cas9-generated cse1 and cse2 single mutants had a wild-type lignin level. Nevertheless, CSE1 and CSE2 are not completely redundant, as both single mutants accumulated caffeoyl shikimate. In contrast, the cse1 cse2 double mutants had a 35% reduction in lignin and associated growth penalty. The reduced lignin content translated into a four-fold increase in cellulose-to-glucose conversion upon limited saccharification. Phenolic profiling of the double mutants revealed large metabolic shifts, including an accumulation of p-coumaroyl, 5-hydroxyferuloyl, feruloyl and sinapoyl shikimate, in addition to caffeoyl shikimate. This indicates that the CSEs have a broad substrate specificity, which was confirmed by in vitro enzyme kinetics. Taken together, our results suggest an alternative path within the phenylpropanoid pathway at the level of the hydroxycinnamoyl shikimates, and show that CSE is a promising target to improve plants for the biorefinery.
Keywords
Biotechnology, Agronomy and Crop Science, Plant Science, poplar, CRISPR-Cas9, CSE, lignin, phenylpropanoids, metabolic engineering, LIGNIN BIOSYNTHESIS, ARABIDOPSIS-THALIANA, DOWN-REGULATION, POPLAR, COA, SACCHARIFICATION, DEPOSITION, REVEALS, ENZYME, GROWTH

Downloads

  • Download
    Published.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 1.82 MB

Citation

Please use this url to cite or link to this publication:

MLA
de Vries, Lisanne, et al. “CRISPR‐Cas9 Editing of Caffeoyl Shikimate Esterase 1 and 2 Shows Their Importance and Partial Redundancy in Lignification in Populus Tremula x P. Alba.” PLANT BIOTECHNOLOGY JOURNAL, vol. 19, no. 11, 2021, pp. 2221–34, doi:10.1111/pbi.13651.
APA
de Vries, L., Brouckaert, M., Alvarenga Chanoca, A., Kim, H., Regner, M. R., Timokhin, V. I., … Boerjan, W. (2021). CRISPR‐Cas9 editing of Caffeoyl Shikimate Esterase 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula x P. alba. PLANT BIOTECHNOLOGY JOURNAL, 19(11), 2221–2234. https://doi.org/10.1111/pbi.13651
Chicago author-date
Vries, Lisanne de, Marlies Brouckaert, Alexandra Alvarenga Chanoca, Hoon Kim, Matthew R. Regner, Vitaliy I. Timokhin, Yi Sun, et al. 2021. “CRISPR‐Cas9 Editing of Caffeoyl Shikimate Esterase 1 and 2 Shows Their Importance and Partial Redundancy in Lignification in Populus Tremula x P. Alba.” PLANT BIOTECHNOLOGY JOURNAL 19 (11): 2221–34. https://doi.org/10.1111/pbi.13651.
Chicago author-date (all authors)
de Vries, Lisanne, Marlies Brouckaert, Alexandra Alvarenga Chanoca, Hoon Kim, Matthew R. Regner, Vitaliy I. Timokhin, Yi Sun, Barbara De Meester, Jan Van Doorsselaere, Geert Goeminne, Vincent L. Chiang, Jack P. Wang, John Ralph, Kris Morreel, Ruben Vanholme, and Wout Boerjan. 2021. “CRISPR‐Cas9 Editing of Caffeoyl Shikimate Esterase 1 and 2 Shows Their Importance and Partial Redundancy in Lignification in Populus Tremula x P. Alba.” PLANT BIOTECHNOLOGY JOURNAL 19 (11): 2221–2234. doi:10.1111/pbi.13651.
Vancouver
1.
de Vries L, Brouckaert M, Alvarenga Chanoca A, Kim H, Regner MR, Timokhin VI, et al. CRISPR‐Cas9 editing of Caffeoyl Shikimate Esterase 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula x P. alba. PLANT BIOTECHNOLOGY JOURNAL. 2021;19(11):2221–34.
IEEE
[1]
L. de Vries et al., “CRISPR‐Cas9 editing of Caffeoyl Shikimate Esterase 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula x P. alba,” PLANT BIOTECHNOLOGY JOURNAL, vol. 19, no. 11, pp. 2221–2234, 2021.
@article{8713730,
  abstract     = {{Lignins are cell-wall-located aromatic polymers that provide strength and hydrophobicity to woody tissues. Lignin monomers are synthesized via the phenylpropanoid pathway, wherein CAFFEOYL SHIKIMATE ESTERASE (CSE) converts caffeoyl shikimate into caffeic acid. Here, we explored the role of the two CSE homologs in poplar (Populus tremula x P. alba). Reporter lines showed that the expression conferred by both CSE1 and CSE2 promoters is similar. CRISPR-Cas9-generated cse1 and cse2 single mutants had a wild-type lignin level. Nevertheless, CSE1 and CSE2 are not completely redundant, as both single mutants accumulated caffeoyl shikimate. In contrast, the cse1 cse2 double mutants had a 35% reduction in lignin and associated growth penalty. The reduced lignin content translated into a four-fold increase in cellulose-to-glucose conversion upon limited saccharification. Phenolic profiling of the double mutants revealed large metabolic shifts, including an accumulation of p-coumaroyl, 5-hydroxyferuloyl, feruloyl and sinapoyl shikimate, in addition to caffeoyl shikimate. This indicates that the CSEs have a broad substrate specificity, which was confirmed by in vitro enzyme kinetics. Taken together, our results suggest an alternative path within the phenylpropanoid pathway at the level of the hydroxycinnamoyl shikimates, and show that CSE is a promising target to improve plants for the biorefinery.}},
  author       = {{de Vries, Lisanne and Brouckaert, Marlies and Alvarenga Chanoca, Alexandra and Kim, Hoon and Regner, Matthew R. and Timokhin, Vitaliy I. and Sun, Yi and De Meester, Barbara and Van Doorsselaere, Jan and Goeminne, Geert and Chiang, Vincent L. and Wang, Jack P. and Ralph, John and Morreel, Kris and Vanholme, Ruben and Boerjan, Wout}},
  issn         = {{1467-7644}},
  journal      = {{PLANT BIOTECHNOLOGY JOURNAL}},
  keywords     = {{Biotechnology,Agronomy and Crop Science,Plant Science,poplar,CRISPR-Cas9,CSE,lignin,phenylpropanoids,metabolic engineering,LIGNIN BIOSYNTHESIS,ARABIDOPSIS-THALIANA,DOWN-REGULATION,POPLAR,COA,SACCHARIFICATION,DEPOSITION,REVEALS,ENZYME,GROWTH}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{2221--2234}},
  title        = {{CRISPR‐Cas9 editing of Caffeoyl Shikimate Esterase 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula x P. alba}},
  url          = {{http://doi.org/10.1111/pbi.13651}},
  volume       = {{19}},
  year         = {{2021}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: