Genetic listeria monocytogenes types in the pork processing plant environment : from occasional introduction to plausible persistence in harborage sites
- Author
- Niels Demaître, Geertrui Rasschaert (UGent) , Lieven De Zutter (UGent) , Annemie Geeraerd and Koen De Reu
- Organization
- Abstract
- The purpose of this study was to investigate the L. monocytogenes occurrence and genetic diversity in three Belgian pork cutting plants. We specifically aim to identify harborage sites and niche locations where this pathogen might occur. A total of 868 samples were taken from a large diversity of food and non-food contact surfaces after cleaning and disinfection (C&D) and during processing. A total of 13% (110/868) of environmental samples tested positive for L. monocytogenes. When looking in more detail, zone 3 non-food contact surfaces were contaminated more often (26%; 72/278) at typical harborage sites, such as floors, drains, and cleaning materials. Food contact surfaces (zone 1) were less frequently contaminated (6%; 25/436), also after C&D. PFGE analysis exhibited low genetic heterogeneity, revealing 11 assigned clonal complexes (CC), four of which (CC8, CC9, CC31, and CC121) were predominant and widespread. Our data suggest (i) the occasional introduction and repeated contamination and/or (ii) the establishment of some persistent meat-adapted clones in all cutting plants. Further, we highlight the importance of well-designed extensive sampling programs combined with genetic characterization to help these facilities take corrective actions to prevent transfer of this pathogen from the environment to the meat.
- Keywords
- MEAT, CONTAMINATION, IDENTIFICATION, STRAINS, PREVALENCE, DIVERSITY, SEROTYPE, FOODS, BEEF, PIG, Listeria monocytogenes, cutting plant, environment, persistence, sampling plan, after C&D, during production
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8712445
- MLA
- Demaître, Niels, et al. “Genetic Listeria Monocytogenes Types in the Pork Processing Plant Environment : From Occasional Introduction to Plausible Persistence in Harborage Sites.” PATHOGENS, vol. 10, no. 6, 2021, doi:10.3390/pathogens10060717.
- APA
- Demaître, N., Rasschaert, G., De Zutter, L., Geeraerd, A., & De Reu, K. (2021). Genetic listeria monocytogenes types in the pork processing plant environment : from occasional introduction to plausible persistence in harborage sites. PATHOGENS, 10(6). https://doi.org/10.3390/pathogens10060717
- Chicago author-date
- Demaître, Niels, Geertrui Rasschaert, Lieven De Zutter, Annemie Geeraerd, and Koen De Reu. 2021. “Genetic Listeria Monocytogenes Types in the Pork Processing Plant Environment : From Occasional Introduction to Plausible Persistence in Harborage Sites.” PATHOGENS 10 (6). https://doi.org/10.3390/pathogens10060717.
- Chicago author-date (all authors)
- Demaître, Niels, Geertrui Rasschaert, Lieven De Zutter, Annemie Geeraerd, and Koen De Reu. 2021. “Genetic Listeria Monocytogenes Types in the Pork Processing Plant Environment : From Occasional Introduction to Plausible Persistence in Harborage Sites.” PATHOGENS 10 (6). doi:10.3390/pathogens10060717.
- Vancouver
- 1.Demaître N, Rasschaert G, De Zutter L, Geeraerd A, De Reu K. Genetic listeria monocytogenes types in the pork processing plant environment : from occasional introduction to plausible persistence in harborage sites. PATHOGENS. 2021;10(6).
- IEEE
- [1]N. Demaître, G. Rasschaert, L. De Zutter, A. Geeraerd, and K. De Reu, “Genetic listeria monocytogenes types in the pork processing plant environment : from occasional introduction to plausible persistence in harborage sites,” PATHOGENS, vol. 10, no. 6, 2021.
@article{8712445,
abstract = {{The purpose of this study was to investigate the L. monocytogenes occurrence and genetic diversity in three Belgian pork cutting plants. We specifically aim to identify harborage sites and niche locations where this pathogen might occur. A total of 868 samples were taken from a large diversity of food and non-food contact surfaces after cleaning and disinfection (C&D) and during processing. A total of 13% (110/868) of environmental samples tested positive for L. monocytogenes. When looking in more detail, zone 3 non-food contact surfaces were contaminated more often (26%; 72/278) at typical harborage sites, such as floors, drains, and cleaning materials. Food contact surfaces (zone 1) were less frequently contaminated (6%; 25/436), also after C&D. PFGE analysis exhibited low genetic heterogeneity, revealing 11 assigned clonal complexes (CC), four of which (CC8, CC9, CC31, and CC121) were predominant and widespread. Our data suggest (i) the occasional introduction and repeated contamination and/or (ii) the establishment of some persistent meat-adapted clones in all cutting plants. Further, we highlight the importance of well-designed extensive sampling programs combined with genetic characterization to help these facilities take corrective actions to prevent transfer of this pathogen from the environment to the meat.}},
articleno = {{717}},
author = {{Demaître, Niels and Rasschaert, Geertrui and De Zutter, Lieven and Geeraerd, Annemie and De Reu, Koen}},
issn = {{2076-0817}},
journal = {{PATHOGENS}},
keywords = {{MEAT,CONTAMINATION,IDENTIFICATION,STRAINS,PREVALENCE,DIVERSITY,SEROTYPE,FOODS,BEEF,PIG,Listeria monocytogenes,cutting plant,environment,persistence,sampling plan,after C&D,during production}},
language = {{eng}},
number = {{6}},
pages = {{12}},
title = {{Genetic listeria monocytogenes types in the pork processing plant environment : from occasional introduction to plausible persistence in harborage sites}},
url = {{http://doi.org/10.3390/pathogens10060717}},
volume = {{10}},
year = {{2021}},
}
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