Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana
- Author
- Hongjiang Li (UGent) , Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof Wabnik (UGent) , Riet De Rycke (UGent) , Walter A. Kaufmann, Daniel Guetl, Ricardo Tejos Ulloa (UGent) , Peter Grones, Meiyu Ke, Xu Chen (UGent) , Jan Dettmer (UGent) and Jiri Friml
- Organization
- Abstract
- Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization ofArabidopsisPIN-FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear. Here, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze-fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains. Pharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell-wall components as well as connections between the cell wall and the plasma membrane. This study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems.
- Keywords
- DEPENDENT AUXIN GRADIENTS, APICAL-BASAL AXIS, PLASMA-MEMBRANE, SYNTHASE, COMPLEXES, INTRACELLULAR TRAFFICKING, HELICAL GROWTH, ARABIDOPSIS, PROTEIN, TRANSPORT, PHOSPHORYLATION, Arabidopsis, auxin, cell wall, cluster, cytoskeleton, PIN, PIP5K, polarity
Downloads
-
4394 20Li.pdf
- full text (Published version)
- |
- open access
- |
- |
- 4.06 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8712086
- MLA
- Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” NEW PHYTOLOGIST, vol. 229, no. 1, 2021, pp. 351–69, doi:10.1111/nph.16887.
- APA
- Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto, S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. NEW PHYTOLOGIST, 229(1), 351–369. https://doi.org/10.1111/nph.16887
- Chicago author-date
- Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof Wabnik, et al. 2021. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” NEW PHYTOLOGIST 229 (1): 351–69. https://doi.org/10.1111/nph.16887.
- Chicago author-date (all authors)
- Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof Wabnik, Riet De Rycke, Walter A. Kaufmann, Daniel Guetl, Ricardo Tejos Ulloa, Peter Grones, Meiyu Ke, Xu Chen, Jan Dettmer, and Jiri Friml. 2021. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” NEW PHYTOLOGIST 229 (1): 351–369. doi:10.1111/nph.16887.
- Vancouver
- 1.Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, et al. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. NEW PHYTOLOGIST. 2021;229(1):351–69.
- IEEE
- [1]H. Li et al., “Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana,” NEW PHYTOLOGIST, vol. 229, no. 1, pp. 351–369, 2021.
@article{8712086,
abstract = {{Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization ofArabidopsisPIN-FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear. Here, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze-fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains. Pharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell-wall components as well as connections between the cell wall and the plasma membrane. This study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems.}},
author = {{Li, Hongjiang and von Wangenheim, Daniel and Zhang, Xixi and Tan, Shutang and Darwish-Miranda, Nasser and Naramoto, Satoshi and Wabnik, Krzysztof and De Rycke, Riet and Kaufmann, Walter A. and Guetl, Daniel and Tejos Ulloa, Ricardo and Grones, Peter and Ke, Meiyu and Chen, Xu and Dettmer, Jan and Friml, Jiri}},
issn = {{0028-646X}},
journal = {{NEW PHYTOLOGIST}},
keywords = {{DEPENDENT AUXIN GRADIENTS,APICAL-BASAL AXIS,PLASMA-MEMBRANE,SYNTHASE,COMPLEXES,INTRACELLULAR TRAFFICKING,HELICAL GROWTH,ARABIDOPSIS,PROTEIN,TRANSPORT,PHOSPHORYLATION,Arabidopsis,auxin,cell wall,cluster,cytoskeleton,PIN,PIP5K,polarity}},
language = {{eng}},
number = {{1}},
pages = {{351--369}},
title = {{Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana}},
url = {{http://doi.org/10.1111/nph.16887}},
volume = {{229}},
year = {{2021}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: