Advanced search
1 file | 1.87 MB Add to list

Effect of subinhibitory exposure to quaternary ammonium compounds on the ciprofloxacin susceptibility ofEscherichia colistrains in animal husbandry

Author
Organization
Abstract
Background Quaternary ammonium compound based disinfectants are commonly used in pig and poultry husbandry to maintain farm hygiene. However, studies have shown that subinhibitory concentrations of these disinfectants may increase antibiotic resistance. Investigation of antibiotic susceptibility is usually assessed via the microbroth dilution method, although this conventional culture-based technique only provides information on the bacteriostatic activity of an antimicrobial agent. Therefore, experiments were performed to investigate the effect of prior benzalkonium chloride (BKC) exposure on the viability of subsequent ciprofloxacin (CIP) treatedEscherichia coli. Results Following CIP treatment, bacterial cell counts were significantly higher after exposure to a subinhibitory BKC concentration than without BKC exposure. The flow cytometric results suggested a BKC-dependent onset of membrane damage and loss of membrane potential. Conclusion Our results indicate a lower bactericidal effect of CIP treatment on BKC-exposedE. coliisolates compared to unexposedE. coliisolates.
Keywords
BENZALKONIUM CHLORIDE, MEMBRANE-PERMEABILITY, CROSS-RESISTANCE, FLOW-CYTOMETRY, BACTERIAL, EFFLUX, MECHANISMS, BIOCIDES, DISINFECTANTS, ANTIBIOTICS, Biocide exposure, Antibiotic susceptibility, Viability, Culturability, Benzalkonium chloride, Ciprofloxacin, Escherichia coli, Flow cytometry, Quaternary ammonium compound

Downloads

  • Effect of subinhibitory JDW BMC Microbiol 2020.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 1.87 MB

Citation

Please use this url to cite or link to this publication:

MLA
Maertens, H., et al. “Effect of Subinhibitory Exposure to Quaternary Ammonium Compounds on the Ciprofloxacin Susceptibility OfEscherichia Colistrains in Animal Husbandry.” BMC MICROBIOLOGY, vol. 20, no. 1, 2020, doi:10.1186/s12866-020-01818-3.
APA
Maertens, H., Demeyere, K., De Reu, K., Dewulf, J., Vanhauteghem, D., Van Coillie, E., & Meyer, E. (2020). Effect of subinhibitory exposure to quaternary ammonium compounds on the ciprofloxacin susceptibility ofEscherichia colistrains in animal husbandry. BMC MICROBIOLOGY, 20(1). https://doi.org/10.1186/s12866-020-01818-3
Chicago author-date
Maertens, H., Kristel Demeyere, K. De Reu, Jeroen Dewulf, Donna Vanhauteghem, E. Van Coillie, and Evelyne Meyer. 2020. “Effect of Subinhibitory Exposure to Quaternary Ammonium Compounds on the Ciprofloxacin Susceptibility OfEscherichia Colistrains in Animal Husbandry.” BMC MICROBIOLOGY 20 (1). https://doi.org/10.1186/s12866-020-01818-3.
Chicago author-date (all authors)
Maertens, H., Kristel Demeyere, K. De Reu, Jeroen Dewulf, Donna Vanhauteghem, E. Van Coillie, and Evelyne Meyer. 2020. “Effect of Subinhibitory Exposure to Quaternary Ammonium Compounds on the Ciprofloxacin Susceptibility OfEscherichia Colistrains in Animal Husbandry.” BMC MICROBIOLOGY 20 (1). doi:10.1186/s12866-020-01818-3.
Vancouver
1.
Maertens H, Demeyere K, De Reu K, Dewulf J, Vanhauteghem D, Van Coillie E, et al. Effect of subinhibitory exposure to quaternary ammonium compounds on the ciprofloxacin susceptibility ofEscherichia colistrains in animal husbandry. BMC MICROBIOLOGY. 2020;20(1).
IEEE
[1]
H. Maertens et al., “Effect of subinhibitory exposure to quaternary ammonium compounds on the ciprofloxacin susceptibility ofEscherichia colistrains in animal husbandry,” BMC MICROBIOLOGY, vol. 20, no. 1, 2020.
@article{8711582,
  abstract     = {{Background Quaternary ammonium compound based disinfectants are commonly used in pig and poultry husbandry to maintain farm hygiene. However, studies have shown that subinhibitory concentrations of these disinfectants may increase antibiotic resistance. Investigation of antibiotic susceptibility is usually assessed via the microbroth dilution method, although this conventional culture-based technique only provides information on the bacteriostatic activity of an antimicrobial agent. Therefore, experiments were performed to investigate the effect of prior benzalkonium chloride (BKC) exposure on the viability of subsequent ciprofloxacin (CIP) treatedEscherichia coli. Results Following CIP treatment, bacterial cell counts were significantly higher after exposure to a subinhibitory BKC concentration than without BKC exposure. The flow cytometric results suggested a BKC-dependent onset of membrane damage and loss of membrane potential. Conclusion Our results indicate a lower bactericidal effect of CIP treatment on BKC-exposedE. coliisolates compared to unexposedE. coliisolates.}},
  articleno    = {{155}},
  author       = {{Maertens, H. and Demeyere, Kristel and De Reu, K. and Dewulf, Jeroen and Vanhauteghem, Donna and Van Coillie, E. and Meyer, Evelyne}},
  issn         = {{1471-2180}},
  journal      = {{BMC MICROBIOLOGY}},
  keywords     = {{BENZALKONIUM CHLORIDE,MEMBRANE-PERMEABILITY,CROSS-RESISTANCE,FLOW-CYTOMETRY,BACTERIAL,EFFLUX,MECHANISMS,BIOCIDES,DISINFECTANTS,ANTIBIOTICS,Biocide exposure,Antibiotic susceptibility,Viability,Culturability,Benzalkonium chloride,Ciprofloxacin,Escherichia coli,Flow cytometry,Quaternary ammonium compound}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{11}},
  title        = {{Effect of subinhibitory exposure to quaternary ammonium compounds on the ciprofloxacin susceptibility ofEscherichia colistrains in animal husbandry}},
  url          = {{http://dx.doi.org/10.1186/s12866-020-01818-3}},
  volume       = {{20}},
  year         = {{2020}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: