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Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection

Joke Elskens (UGent) and Annemieke Madder (UGent)
(2021) RSC CHEMICAL BIOLOGY. 2(2). p.410-422
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Abstract
Understanding the intricate interaction pattern of nucleic acids with other molecules is essential to gain further insight in biological processes and disease mechanisms. To this end, a multitude of hybridization-based assays have been designed that rely on the non-covalent recognition between complementary nucleic acid sequences. However, the ephemeral nature of these interactions complicates straightforward analysis as low efficiency and specificity are rule rather than exception. By covalently locking nucleic acid interactions by means of a crosslinking agent, the overall efficiency, specificity and selectivity of hybridization-based assays could be increased. In this mini-review we highlight methodologies that exploit the use of crosslinker-modified nucleic acid probes for interstrand nucleic acid crosslinking with the objective to study, detect and identify important targets as well as nucleic acid sequences that can be considered relevant biomarkers. We emphasize on the usefulness and advantages of crosslinking agents and elaborate on the chemistry behind the crosslinking reactions they induce.
Keywords
LONG NONCODING RNAS, LINKING REACTION, PHOTOAFFINITY PROBES, COVALENT, CAPTURE, ABASIC SITES, DNA, PROTEIN, OLIGONUCLEOTIDE, COUMARIN, DUPLEX

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Citation

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MLA
Elskens, Joke, and Annemieke Madder. “Crosslinker-Modified Nucleic Acid Probes for Improved Target Identification and Biomarker Detection.” RSC CHEMICAL BIOLOGY, vol. 2, no. 2, 2021, pp. 410–22, doi:10.1039/d0cb00236d.
APA
Elskens, J., & Madder, A. (2021). Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection. RSC CHEMICAL BIOLOGY, 2(2), 410–422. https://doi.org/10.1039/d0cb00236d
Chicago author-date
Elskens, Joke, and Annemieke Madder. 2021. “Crosslinker-Modified Nucleic Acid Probes for Improved Target Identification and Biomarker Detection.” RSC CHEMICAL BIOLOGY 2 (2): 410–22. https://doi.org/10.1039/d0cb00236d.
Chicago author-date (all authors)
Elskens, Joke, and Annemieke Madder. 2021. “Crosslinker-Modified Nucleic Acid Probes for Improved Target Identification and Biomarker Detection.” RSC CHEMICAL BIOLOGY 2 (2): 410–422. doi:10.1039/d0cb00236d.
Vancouver
1.
Elskens J, Madder A. Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection. RSC CHEMICAL BIOLOGY. 2021;2(2):410–22.
IEEE
[1]
J. Elskens and A. Madder, “Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection,” RSC CHEMICAL BIOLOGY, vol. 2, no. 2, pp. 410–422, 2021.
@article{8711240,
  abstract     = {{Understanding the intricate interaction pattern of nucleic acids with other molecules is essential to gain further insight in biological processes and disease mechanisms. To this end, a multitude of hybridization-based assays have been designed that rely on the non-covalent recognition between complementary nucleic acid sequences. However, the ephemeral nature of these interactions complicates straightforward analysis as low efficiency and specificity are rule rather than exception. By covalently locking nucleic acid interactions by means of a crosslinking agent, the overall efficiency, specificity and selectivity of hybridization-based assays could be increased. In this mini-review we highlight methodologies that exploit the use of crosslinker-modified nucleic acid probes for interstrand nucleic acid crosslinking with the objective to study, detect and identify important targets as well as nucleic acid sequences that can be considered relevant biomarkers. We emphasize on the usefulness and advantages of crosslinking agents and elaborate on the chemistry behind the crosslinking reactions they induce.}},
  author       = {{Elskens, Joke and Madder, Annemieke}},
  issn         = {{2633-0679}},
  journal      = {{RSC CHEMICAL BIOLOGY}},
  keywords     = {{LONG NONCODING RNAS,LINKING REACTION,PHOTOAFFINITY PROBES,COVALENT,CAPTURE,ABASIC SITES,DNA,PROTEIN,OLIGONUCLEOTIDE,COUMARIN,DUPLEX}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{410--422}},
  title        = {{Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection}},
  url          = {{http://dx.doi.org/10.1039/d0cb00236d}},
  volume       = {{2}},
  year         = {{2021}},
}

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