A molecular toolkit for the green seaweed Ulva mutabilis
- Author
- Jonas Blomme (UGent) , Xiaojie Liu, Thomas B. Jacobs (UGent) and Olivier De Clerck (UGent)
- Organization
- Abstract
- The green seaweed Ulva mutabilis is an ecologically important marine primary producer as well as a promising cash crop cultivated for multiple uses. Despite its importance, several molecular tools are still needed to better understand seaweed biology. Here, we report the development of a flexible and modular molecular cloning toolkit for the green seaweed U. mutabilis based on a Golden Gate cloning system. The toolkit presently contains 125 entry vectors, 26 destination vectors, and 107 functionally validated expression vectors. We demonstrate the importance of endogenous regulatory sequences for transgene expression and characterize three endogenous promoters suitable to drive transgene expression. We describe two vector architectures to express transgenes via two expression cassettes or a bicistronic approach. The majority of selected transformants (50%-80%) consistently give clear visual transgene expression. Furthermore, we made different marker lines for intracellular compartments after evaluating 13 transit peptides and 11 tagged endogenous Ulm genes. Our molecular toolkit enables the study of Ulm gain-of-function lines and paves the way for gene characterization and large-scale functional genomics studies in a green seaweed.
- Keywords
- Plant Science, Genetics, Physiology, COMPLETE MITOCHONDRIAL GENOME, CHLAMYDOMONAS-REINHARDTII, MACROALGAL BLOOMS, GENE-EXPRESSION, GAMETE RELEASE, REPORTER GENE, YELLOW SEA, SHED LIGHT, CHLOROPHYTA, PROTEIN
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8710583
- MLA
- Blomme, Jonas, et al. “A Molecular Toolkit for the Green Seaweed Ulva Mutabilis.” PLANT PHYSIOLOGY, vol. 186, no. 3, 2021, pp. 1442–54, doi:10.1093/plphys/kiab185.
- APA
- Blomme, J., Liu, X., Jacobs, T. B., & De Clerck, O. (2021). A molecular toolkit for the green seaweed Ulva mutabilis. PLANT PHYSIOLOGY, 186(3), 1442–1454. https://doi.org/10.1093/plphys/kiab185
- Chicago author-date
- Blomme, Jonas, Xiaojie Liu, Thomas B. Jacobs, and Olivier De Clerck. 2021. “A Molecular Toolkit for the Green Seaweed Ulva Mutabilis.” PLANT PHYSIOLOGY 186 (3): 1442–54. https://doi.org/10.1093/plphys/kiab185.
- Chicago author-date (all authors)
- Blomme, Jonas, Xiaojie Liu, Thomas B. Jacobs, and Olivier De Clerck. 2021. “A Molecular Toolkit for the Green Seaweed Ulva Mutabilis.” PLANT PHYSIOLOGY 186 (3): 1442–1454. doi:10.1093/plphys/kiab185.
- Vancouver
- 1.Blomme J, Liu X, Jacobs TB, De Clerck O. A molecular toolkit for the green seaweed Ulva mutabilis. PLANT PHYSIOLOGY. 2021;186(3):1442–54.
- IEEE
- [1]J. Blomme, X. Liu, T. B. Jacobs, and O. De Clerck, “A molecular toolkit for the green seaweed Ulva mutabilis,” PLANT PHYSIOLOGY, vol. 186, no. 3, pp. 1442–1454, 2021.
@article{8710583,
abstract = {{The green seaweed Ulva mutabilis is an ecologically important marine primary producer as well as a promising cash crop cultivated for multiple uses. Despite its importance, several molecular tools are still needed to better understand seaweed biology. Here, we report the development of a flexible and modular molecular cloning toolkit for the green seaweed U. mutabilis based on a Golden Gate cloning system. The toolkit presently contains 125 entry vectors, 26 destination vectors, and 107 functionally validated expression vectors. We demonstrate the importance of endogenous regulatory sequences for transgene expression and characterize three endogenous promoters suitable to drive transgene expression. We describe two vector architectures to express transgenes via two expression cassettes or a bicistronic approach. The majority of selected transformants (50%-80%) consistently give clear visual transgene expression. Furthermore, we made different marker lines for intracellular compartments after evaluating 13 transit peptides and 11 tagged endogenous Ulm genes. Our molecular toolkit enables the study of Ulm gain-of-function lines and paves the way for gene characterization and large-scale functional genomics studies in a green seaweed.}},
author = {{Blomme, Jonas and Liu, Xiaojie and Jacobs, Thomas B. and De Clerck, Olivier}},
issn = {{0032-0889}},
journal = {{PLANT PHYSIOLOGY}},
keywords = {{Plant Science,Genetics,Physiology,COMPLETE MITOCHONDRIAL GENOME,CHLAMYDOMONAS-REINHARDTII,MACROALGAL BLOOMS,GENE-EXPRESSION,GAMETE RELEASE,REPORTER GENE,YELLOW SEA,SHED LIGHT,CHLOROPHYTA,PROTEIN}},
language = {{eng}},
number = {{3}},
pages = {{1442--1454}},
title = {{A molecular toolkit for the green seaweed Ulva mutabilis}},
url = {{http://doi.org/10.1093/plphys/kiab185}},
volume = {{186}},
year = {{2021}},
}
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