Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization
- Author
- Ana Rita Pombo Antunes, Isabelle Scheyltjens, Francesca Lodi, Julie Messiaen, Asier Antoranz, Johnny Duerinck, Daliya Kancheva, Liesbet Martens (UGent) , Karen De Vlaminck, Hannah Van Hove, Signe Schmidt Kjolner Hansen, Francesca Maria Bosisio, Koen Van der Borght, Steven De Vleeschouwer, Raf Sciot, Luc Bouwens, Michiel Verfaillie, Niels Vandamme (UGent) , Roosmarijn Vandenbroucke (UGent) , Olivier De Wever (UGent) , Yvan Saeys (UGent) , Martin Guilliams (UGent) , Conny Gysemans, Bart Neyns, Frederik De Smet, Diether Lambrechts, Jo A. Van Ginderachter and Kiavash Movahedi
- Organization
- Abstract
- Glioblastomas are aggressive primary brain cancers that recur as therapy-resistant tumors. Myeloid cells control glioblastoma malignancy, but their dynamics during disease progression remain poorly understood. Here, we employed single-cell RNA sequencing and CITE-seq to map the glioblastoma immune landscape in mouse tumors and in patients with newly diagnosed disease or recurrence. This revealed a large and diverse myeloid compartment, with dendritic cell and macrophage populations that were conserved across species and dynamic across disease stages. Tumor-associated macrophages (TAMs) consisted of microglia- or monocyte-derived populations, with both exhibiting additional heterogeneity, including subsets with conserved lipid and hypoxic signatures. Microglia- and monocyte-derived TAMs were self-renewing populations that competed for space and could be depleted via CSF1R blockade. Microglia-derived TAMs were predominant in newly diagnosed tumors, but were outnumbered by monocyte-derived TAMs following recurrence, especially in hypoxic tumor environments. Our results unravel the glioblastoma myeloid landscape and provide a framework for future therapeutic interventions.
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8709709
- MLA
- Antunes, Ana Rita Pombo, et al. “Single-Cell Profiling of Myeloid Cells in Glioblastoma across Species and Disease Stage Reveals Macrophage Competition and Specialization.” NATURE NEUROSCIENCE, vol. 24, no. 4, 2021, pp. 595–610, doi:10.1038/s41593-020-00789-y.
- APA
- Antunes, A. R. P., Scheyltjens, I., Lodi, F., Messiaen, J., Antoranz, A., Duerinck, J., … Movahedi, K. (2021). Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization. NATURE NEUROSCIENCE, 24(4), 595–610. https://doi.org/10.1038/s41593-020-00789-y
- Chicago author-date
- Antunes, Ana Rita Pombo, Isabelle Scheyltjens, Francesca Lodi, Julie Messiaen, Asier Antoranz, Johnny Duerinck, Daliya Kancheva, et al. 2021. “Single-Cell Profiling of Myeloid Cells in Glioblastoma across Species and Disease Stage Reveals Macrophage Competition and Specialization.” NATURE NEUROSCIENCE 24 (4): 595–610. https://doi.org/10.1038/s41593-020-00789-y.
- Chicago author-date (all authors)
- Antunes, Ana Rita Pombo, Isabelle Scheyltjens, Francesca Lodi, Julie Messiaen, Asier Antoranz, Johnny Duerinck, Daliya Kancheva, Liesbet Martens, Karen De Vlaminck, Hannah Van Hove, Signe Schmidt Kjolner Hansen, Francesca Maria Bosisio, Koen Van der Borght, Steven De Vleeschouwer, Raf Sciot, Luc Bouwens, Michiel Verfaillie, Niels Vandamme, Roosmarijn Vandenbroucke, Olivier De Wever, Yvan Saeys, Martin Guilliams, Conny Gysemans, Bart Neyns, Frederik De Smet, Diether Lambrechts, Jo A. Van Ginderachter, and Kiavash Movahedi. 2021. “Single-Cell Profiling of Myeloid Cells in Glioblastoma across Species and Disease Stage Reveals Macrophage Competition and Specialization.” NATURE NEUROSCIENCE 24 (4): 595–610. doi:10.1038/s41593-020-00789-y.
- Vancouver
- 1.Antunes ARP, Scheyltjens I, Lodi F, Messiaen J, Antoranz A, Duerinck J, et al. Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization. NATURE NEUROSCIENCE. 2021;24(4):595–610.
- IEEE
- [1]A. R. P. Antunes et al., “Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization,” NATURE NEUROSCIENCE, vol. 24, no. 4, pp. 595–610, 2021.
@article{8709709,
abstract = {{Glioblastomas are aggressive primary brain cancers that recur as therapy-resistant tumors. Myeloid cells control glioblastoma malignancy, but their dynamics during disease progression remain poorly understood. Here, we employed single-cell RNA sequencing and CITE-seq to map the glioblastoma immune landscape in mouse tumors and in patients with newly diagnosed disease or recurrence. This revealed a large and diverse myeloid compartment, with dendritic cell and macrophage populations that were conserved across species and dynamic across disease stages. Tumor-associated macrophages (TAMs) consisted of microglia- or monocyte-derived populations, with both exhibiting additional heterogeneity, including subsets with conserved lipid and hypoxic signatures. Microglia- and monocyte-derived TAMs were self-renewing populations that competed for space and could be depleted via CSF1R blockade. Microglia-derived TAMs were predominant in newly diagnosed tumors, but were outnumbered by monocyte-derived TAMs following recurrence, especially in hypoxic tumor environments. Our results unravel the glioblastoma myeloid landscape and provide a framework for future therapeutic interventions.}},
author = {{Antunes, Ana Rita Pombo and Scheyltjens, Isabelle and Lodi, Francesca and Messiaen, Julie and Antoranz, Asier and Duerinck, Johnny and Kancheva, Daliya and Martens, Liesbet and De Vlaminck, Karen and Van Hove, Hannah and Hansen, Signe Schmidt Kjolner and Bosisio, Francesca Maria and Van der Borght, Koen and De Vleeschouwer, Steven and Sciot, Raf and Bouwens, Luc and Verfaillie, Michiel and Vandamme, Niels and Vandenbroucke, Roosmarijn and De Wever, Olivier and Saeys, Yvan and Guilliams, Martin and Gysemans, Conny and Neyns, Bart and De Smet, Frederik and Lambrechts, Diether and Van Ginderachter, Jo A. and Movahedi, Kiavash}},
issn = {{1097-6256}},
journal = {{NATURE NEUROSCIENCE}},
language = {{eng}},
number = {{4}},
pages = {{595--610}},
title = {{Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization}},
url = {{http://doi.org/10.1038/s41593-020-00789-y}},
volume = {{24}},
year = {{2021}},
}
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