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The efficacy and effect on gut microbiota of an aflatoxin binder and a fumonisin esterase using an in vitro simulator of the human intestinal microbial ecosystem (SHIME®)

Kaat Neckermann (UGent) , Gregor Claus (UGent) , Siegrid De Baere (UGent) , Gunther Antonissen (UGent) , Sarah Lebrun, Céline Gemmi, Bernard Taminiau, Caroline Douny, Marie-Louise Scippo, Dian Schatzmayr, et al.
(2021) FOOD RESEARCH INTERNATIONAL. 145.
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Abstract
Mycotoxin intoxication is in general an acknowledged and tackled issue in animals. However, in several parts of the world, mycotoxicoses in humans still remain a relevant issue. The efficacy of two mycotoxin detoxifying animal feed additives, an aflatoxin bentonite clay binder and a fumonisin esterase, was investigated in a human child gut model, i.e. the in vitro Simulator of the Human Intestinal Microbial Ecosystem (SHIME (R)). Additionally, the effect of the detoxifiers on gut microbiota was examined in the SHIME. After an initial two weeks of system stabilisation, aflatoxin B1 (AFB1) and fumonisin B1 (FB1) were added to the SHIME diet during one week. Next, the two detoxifiers and mycotoxins were added to the system for an additional week. The AFB1, FB1, hydrolysed FB1 (HFB1), partially hydrolysed FB1a and FB1b concentrations were determined in SHIME samples using a validated ultra-performance liquid chromatography-tandem mass spectrometry method. The short-chain fatty acid (SCFA) concentrations were determined by a validated gas chromatography-mass spectrometry method. Colonic bacterial communities were analysed using metabarcoding, targeting the hypervariable V1-V3 regions of the 16S rRNA genes. The AFB1 and FB1 concentrations significantly decreased after the addition of the detoxifiers. Likewise, the concentration of HFB1 significantly increased. Concentrations of SCFAs remained generally stable throughout the experiment. No major changes in bacterial composition occurred during the experiment. The results demonstrate the promising effect of these detoxifiers in reducing AFB1 and FB1 concentrations in the human intestinal environment, without compromising the gastrointestinal microbiota.
Keywords
Food Science, Mycotoxin, Aflatoxin B1, Fumonisin B1, Bentonite binder, Fumonisin esterase, Fumonisin metabolites, Detoxifiers, Intestinal microbiota, Human intestinal model, Efficacy, In vitro, Toddler SHIME, CALCIUM MONTMORILLONITE CLAY, CHAIN FATTY-ACIDS, MYCOTOXIN CONTAMINATION, DIETARY EXPOSURE, NOVASIL CLAY, HIGH-RISK, B-1, METABOLISM, MODEL, KENYA

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MLA
Neckermann, Kaat, et al. “The Efficacy and Effect on Gut Microbiota of an Aflatoxin Binder and a Fumonisin Esterase Using an in Vitro Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).” FOOD RESEARCH INTERNATIONAL, edited by Anderson Sant’Ana, vol. 145, 2021, doi:10.1016/j.foodres.2021.110395.
APA
Neckermann, K., Claus, G., De Baere, S., Antonissen, G., Lebrun, S., Gemmi, C., … Delcenserie, V. (2021). The efficacy and effect on gut microbiota of an aflatoxin binder and a fumonisin esterase using an in vitro simulator of the human intestinal microbial ecosystem (SHIME®). FOOD RESEARCH INTERNATIONAL, 145. https://doi.org/10.1016/j.foodres.2021.110395
Chicago author-date
Neckermann, Kaat, Gregor Claus, Siegrid De Baere, Gunther Antonissen, Sarah Lebrun, Céline Gemmi, Bernard Taminiau, et al. 2021. “The Efficacy and Effect on Gut Microbiota of an Aflatoxin Binder and a Fumonisin Esterase Using an in Vitro Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).” Edited by Anderson Sant’Ana. FOOD RESEARCH INTERNATIONAL 145. https://doi.org/10.1016/j.foodres.2021.110395.
Chicago author-date (all authors)
Neckermann, Kaat, Gregor Claus, Siegrid De Baere, Gunther Antonissen, Sarah Lebrun, Céline Gemmi, Bernard Taminiau, Caroline Douny, Marie-Louise Scippo, Dian Schatzmayr, James Gathumbi, Silvio Uhlig, Siska Croubels, and Véronique Delcenserie. 2021. “The Efficacy and Effect on Gut Microbiota of an Aflatoxin Binder and a Fumonisin Esterase Using an in Vitro Simulator of the Human Intestinal Microbial Ecosystem (SHIME®).” Ed by. Anderson Sant’Ana. FOOD RESEARCH INTERNATIONAL 145. doi:10.1016/j.foodres.2021.110395.
Vancouver
1.
Neckermann K, Claus G, De Baere S, Antonissen G, Lebrun S, Gemmi C, et al. The efficacy and effect on gut microbiota of an aflatoxin binder and a fumonisin esterase using an in vitro simulator of the human intestinal microbial ecosystem (SHIME®). Sant’Ana A, editor. FOOD RESEARCH INTERNATIONAL. 2021;145.
IEEE
[1]
K. Neckermann et al., “The efficacy and effect on gut microbiota of an aflatoxin binder and a fumonisin esterase using an in vitro simulator of the human intestinal microbial ecosystem (SHIME®),” FOOD RESEARCH INTERNATIONAL, vol. 145, 2021.
@article{8708376,
  abstract     = {{Mycotoxin intoxication is in general an acknowledged and tackled issue in animals. However, in several parts of the world, mycotoxicoses in humans still remain a relevant issue. The efficacy of two mycotoxin detoxifying animal feed additives, an aflatoxin bentonite clay binder and a fumonisin esterase, was investigated in a human child gut model, i.e. the in vitro Simulator of the Human Intestinal Microbial Ecosystem (SHIME (R)). Additionally, the effect of the detoxifiers on gut microbiota was examined in the SHIME. After an initial two weeks of system stabilisation, aflatoxin B1 (AFB1) and fumonisin B1 (FB1) were added to the SHIME diet during one week. Next, the two detoxifiers and mycotoxins were added to the system for an additional week. The AFB1, FB1, hydrolysed FB1 (HFB1), partially hydrolysed FB1a and FB1b concentrations were determined in SHIME samples using a validated ultra-performance liquid chromatography-tandem mass spectrometry method. The short-chain fatty acid (SCFA) concentrations were determined by a validated gas chromatography-mass spectrometry method. Colonic bacterial communities were analysed using metabarcoding, targeting the hypervariable V1-V3 regions of the 16S rRNA genes. The AFB1 and FB1 concentrations significantly decreased after the addition of the detoxifiers. Likewise, the concentration of HFB1 significantly increased. Concentrations of SCFAs remained generally stable throughout the experiment. No major changes in bacterial composition occurred during the experiment. The results demonstrate the promising effect of these detoxifiers in reducing AFB1 and FB1 concentrations in the human intestinal environment, without compromising the gastrointestinal microbiota.}},
  articleno    = {{110395}},
  author       = {{Neckermann, Kaat and Claus, Gregor and De Baere, Siegrid and Antonissen, Gunther and Lebrun, Sarah and Gemmi, Céline and Taminiau, Bernard and Douny, Caroline and Scippo, Marie-Louise and Schatzmayr, Dian and Gathumbi, James and Uhlig, Silvio and Croubels, Siska and Delcenserie, Véronique}},
  editor       = {{Sant'Ana, Anderson}},
  issn         = {{0963-9969}},
  journal      = {{FOOD RESEARCH INTERNATIONAL}},
  keywords     = {{Food Science,Mycotoxin,Aflatoxin B1,Fumonisin B1,Bentonite binder,Fumonisin esterase,Fumonisin metabolites,Detoxifiers,Intestinal microbiota,Human intestinal model,Efficacy,In vitro,Toddler SHIME,CALCIUM MONTMORILLONITE CLAY,CHAIN FATTY-ACIDS,MYCOTOXIN CONTAMINATION,DIETARY EXPOSURE,NOVASIL CLAY,HIGH-RISK,B-1,METABOLISM,MODEL,KENYA}},
  language     = {{eng}},
  pages        = {{14}},
  title        = {{The efficacy and effect on gut microbiota of an aflatoxin binder and a fumonisin esterase using an in vitro simulator of the human intestinal microbial ecosystem (SHIME®)}},
  url          = {{http://dx.doi.org/10.1016/j.foodres.2021.110395}},
  volume       = {{145}},
  year         = {{2021}},
}
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