A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana
- Author
- Anelia Iantcheva, Miroslava Zhiponova, Miglena Revalska, Jefri Heyman (UGent) , Ivayla Dincheva, Ilian Badjakov, Nathan De Geyter (UGent) , Irina Boycheva, Sofie Goormachtig (UGent) and Lieven De Veylder (UGent)
- Organization
- Abstract
- The F-box domain is a conserved structural protein motif that most frequently interacts with the SKP1 protein, the core of the SCFs (SKP1-CULLIN-F-box protein ligase) E3 ubiquitin protein ligases. As part of the SCF complexes, the various F-box proteins recruit substrates for degradation through ubiquitination. In this study, we functionally characterized an F-box gene (MtF-box) identified earlier in a population of Tnt1 retrotransposon-tagged mutants of Medicago truncatula and its Arabidopsis thaliana homolog (AtF-box) using gain- and loss-of-function plants. We highlighted the importance of MtF-box in leaf development of M. truncatula. Protein–protein interaction analyses revealed the 2-isopropylmalate synthase (IPMS) protein as a common interactor partner of MtF-box and AtF-box, being a key enzyme in the biosynthesis pathway of the branched-chain amino acid leucine. For further detailed analysis, we focused on AtF-box and its role during the cell division cycle. Based on this work, we suggest a mechanism for the role of the studied F-box gene in regulation of leucine homeostasis, which is important for growth.
- Keywords
- Plant Science, Cell Biology, General Medicine, Branched-chain amino acid (BCAA) level, M, truncatula and A, thaliana F-box gene, Protein interactor, Transcript level, TANDEM AFFINITY PURIFICATION, LOTUS-JAPONICUS, CELL-DIVISION, AMINO-ACID, GROWTH, BIOSYNTHESIS, EXPRESSION, TOBACCO, PROLIFERATION, TOLERANCE
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8707847
- MLA
- Iantcheva, Anelia, et al. “A Common F-Box Gene Regulates the Leucine Homeostasis of Medicago Truncatula and Arabidopsis Thaliana.” PROTOPLASMA, vol. 259, 2022, pp. 277–90, doi:10.1007/s00709-021-01662-w.
- APA
- Iantcheva, A., Zhiponova, M., Revalska, M., Heyman, J., Dincheva, I., Badjakov, I., … De Veylder, L. (2022). A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana. PROTOPLASMA, 259, 277–290. https://doi.org/10.1007/s00709-021-01662-w
- Chicago author-date
- Iantcheva, Anelia, Miroslava Zhiponova, Miglena Revalska, Jefri Heyman, Ivayla Dincheva, Ilian Badjakov, Nathan De Geyter, Irina Boycheva, Sofie Goormachtig, and Lieven De Veylder. 2022. “A Common F-Box Gene Regulates the Leucine Homeostasis of Medicago Truncatula and Arabidopsis Thaliana.” PROTOPLASMA 259: 277–90. https://doi.org/10.1007/s00709-021-01662-w.
- Chicago author-date (all authors)
- Iantcheva, Anelia, Miroslava Zhiponova, Miglena Revalska, Jefri Heyman, Ivayla Dincheva, Ilian Badjakov, Nathan De Geyter, Irina Boycheva, Sofie Goormachtig, and Lieven De Veylder. 2022. “A Common F-Box Gene Regulates the Leucine Homeostasis of Medicago Truncatula and Arabidopsis Thaliana.” PROTOPLASMA 259: 277–290. doi:10.1007/s00709-021-01662-w.
- Vancouver
- 1.Iantcheva A, Zhiponova M, Revalska M, Heyman J, Dincheva I, Badjakov I, et al. A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana. PROTOPLASMA. 2022;259:277–90.
- IEEE
- [1]A. Iantcheva et al., “A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana,” PROTOPLASMA, vol. 259, pp. 277–290, 2022.
@article{8707847,
abstract = {{The F-box domain is a conserved structural protein motif that most frequently interacts with the SKP1 protein, the core of the SCFs (SKP1-CULLIN-F-box protein ligase) E3 ubiquitin protein ligases. As part of the SCF complexes, the various F-box proteins recruit substrates for degradation through ubiquitination. In this study, we functionally characterized an F-box gene (MtF-box) identified earlier in a population of Tnt1 retrotransposon-tagged mutants of Medicago truncatula and its Arabidopsis thaliana homolog (AtF-box) using gain- and loss-of-function plants. We highlighted the importance of MtF-box in leaf development of M. truncatula. Protein–protein interaction analyses revealed the 2-isopropylmalate synthase (IPMS) protein as a common interactor partner of MtF-box and AtF-box, being a key enzyme in the biosynthesis pathway of the branched-chain amino acid leucine. For further detailed analysis, we focused on AtF-box and its role during the cell division cycle. Based on this work, we suggest a mechanism for the role of the studied F-box gene in regulation of leucine homeostasis, which is important for growth.}},
author = {{Iantcheva, Anelia and Zhiponova, Miroslava and Revalska, Miglena and Heyman, Jefri and Dincheva, Ivayla and Badjakov, Ilian and De Geyter, Nathan and Boycheva, Irina and Goormachtig, Sofie and De Veylder, Lieven}},
issn = {{0033-183X}},
journal = {{PROTOPLASMA}},
keywords = {{Plant Science,Cell Biology,General Medicine,Branched-chain amino acid (BCAA) level,M,truncatula and A,thaliana F-box gene,Protein interactor,Transcript level,TANDEM AFFINITY PURIFICATION,LOTUS-JAPONICUS,CELL-DIVISION,AMINO-ACID,GROWTH,BIOSYNTHESIS,EXPRESSION,TOBACCO,PROLIFERATION,TOLERANCE}},
language = {{eng}},
pages = {{277--290}},
title = {{A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana}},
url = {{http://doi.org/10.1007/s00709-021-01662-w}},
volume = {{259}},
year = {{2022}},
}
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