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Impact of xylanase and glucanase on oligosaccharide formation, carbohydrate fermentation patterns, and nutrient utilization in the gastrointestinal tract of broilers

(2021) ANIMALS. 11(5).
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Abstract
This study aimed at determining how the degradation of cereal non-starch polysaccharides (NSP) by dietary enzymes during feed digestion can influence nutrient digestibility and NSP fermentability in broilers. Ninety-six one-day-old male broilers were assigned to 4 different treatments: control and enzyme-supplemented wheat-based (WC, WE) or maize-based (MC, ME) treatments. Enzyme supplementation with endo-xylanase and endo-glucanase occurred from day 20 onwards. On day 28, digesta samples were collected. Nutrient digestibility, NSP recovery, oligosaccharide profile, and short-chain fatty acids (SCFA) content were determined. Enzyme supplementation in WE resulted in a higher starch (3%; p = 0.004) and protein (5%; p = 0.002) digestion in the ileum compared to WC. Xylanase activity in WE led to in situ formations of arabinoxylan-oligosaccharides consisting of 5 to 26 pentose units in the ileum. This coincided with decreased arabinose (p = 0.059) and xylose (p = 0.036) amounts in the ceca and higher acetate (p = 0.014) and butyrate (p = 0.044) formation in WE compared to WC. Conversely, complete total tract recovery of arabinoxylan in MC and ME suggested poor maize NSP fermentability. Overall, enzyme action improved nutrient digestibility and arabinoxylan fermentability in the wheat-based diet. The lower response of the maize-based diet to enzyme treatment may be related to the recalcitrance of maize arabinoxylan as well as to the high nutritive value of maize.
Keywords
feed enzymes, cereal NSP, xylanase, broilers, oligosaccharide MALDI-TOF-MS profile, digestion, prebiotics, arabinoxylan-oligosaccharides, CHICKENS FED CORN, NONSTARCH POLYSACCHARIDES, MICROBIAL COMPOSITION, EXOGENOUS XYLANASE, GROWTH-PERFORMANCE, GUT MICROBIOTA, BETA-GLUCANASE, WHEAT DIETS, STARCH, ARABINOXYLANS

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MLA
Kouzounis, Dimitrios, et al. “Impact of Xylanase and Glucanase on Oligosaccharide Formation, Carbohydrate Fermentation Patterns, and Nutrient Utilization in the Gastrointestinal Tract of Broilers.” ANIMALS, vol. 11, no. 5, 2021, doi:10.3390/ani11051285.
APA
Kouzounis, D., Hageman, J. A., Soares, N., Michiels, J., & Schols, H. A. (2021). Impact of xylanase and glucanase on oligosaccharide formation, carbohydrate fermentation patterns, and nutrient utilization in the gastrointestinal tract of broilers. ANIMALS, 11(5). https://doi.org/10.3390/ani11051285
Chicago author-date
Kouzounis, Dimitrios, Jos A. Hageman, Natalia Soares, Joris Michiels, and Henk A. Schols. 2021. “Impact of Xylanase and Glucanase on Oligosaccharide Formation, Carbohydrate Fermentation Patterns, and Nutrient Utilization in the Gastrointestinal Tract of Broilers.” ANIMALS 11 (5). https://doi.org/10.3390/ani11051285.
Chicago author-date (all authors)
Kouzounis, Dimitrios, Jos A. Hageman, Natalia Soares, Joris Michiels, and Henk A. Schols. 2021. “Impact of Xylanase and Glucanase on Oligosaccharide Formation, Carbohydrate Fermentation Patterns, and Nutrient Utilization in the Gastrointestinal Tract of Broilers.” ANIMALS 11 (5). doi:10.3390/ani11051285.
Vancouver
1.
Kouzounis D, Hageman JA, Soares N, Michiels J, Schols HA. Impact of xylanase and glucanase on oligosaccharide formation, carbohydrate fermentation patterns, and nutrient utilization in the gastrointestinal tract of broilers. ANIMALS. 2021;11(5).
IEEE
[1]
D. Kouzounis, J. A. Hageman, N. Soares, J. Michiels, and H. A. Schols, “Impact of xylanase and glucanase on oligosaccharide formation, carbohydrate fermentation patterns, and nutrient utilization in the gastrointestinal tract of broilers,” ANIMALS, vol. 11, no. 5, 2021.
@article{8706742,
  abstract     = {{This study aimed at determining how the degradation of cereal non-starch polysaccharides (NSP) by dietary enzymes during feed digestion can influence nutrient digestibility and NSP fermentability in broilers. Ninety-six one-day-old male broilers were assigned to 4 different treatments: control and enzyme-supplemented wheat-based (WC, WE) or maize-based (MC, ME) treatments. Enzyme supplementation with endo-xylanase and endo-glucanase occurred from day 20 onwards. On day 28, digesta samples were collected. Nutrient digestibility, NSP recovery, oligosaccharide profile, and short-chain fatty acids (SCFA) content were determined. Enzyme supplementation in WE resulted in a higher starch (3%; p = 0.004) and protein (5%; p = 0.002) digestion in the ileum compared to WC. Xylanase activity in WE led to in situ formations of arabinoxylan-oligosaccharides consisting of 5 to 26 pentose units in the ileum. This coincided with decreased arabinose (p = 0.059) and xylose (p = 0.036) amounts in the ceca and higher acetate (p = 0.014) and butyrate (p = 0.044) formation in WE compared to WC. Conversely, complete total tract recovery of arabinoxylan in MC and ME suggested poor maize NSP fermentability. Overall, enzyme action improved nutrient digestibility and arabinoxylan fermentability in the wheat-based diet. The lower response of the maize-based diet to enzyme treatment may be related to the recalcitrance of maize arabinoxylan as well as to the high nutritive value of maize.}},
  articleno    = {{1285}},
  author       = {{Kouzounis, Dimitrios and Hageman, Jos A. and Soares, Natalia and Michiels, Joris and Schols, Henk A.}},
  issn         = {{2076-2615}},
  journal      = {{ANIMALS}},
  keywords     = {{feed enzymes,cereal NSP,xylanase,broilers,oligosaccharide MALDI-TOF-MS profile,digestion,prebiotics,arabinoxylan-oligosaccharides,CHICKENS FED CORN,NONSTARCH POLYSACCHARIDES,MICROBIAL COMPOSITION,EXOGENOUS XYLANASE,GROWTH-PERFORMANCE,GUT MICROBIOTA,BETA-GLUCANASE,WHEAT DIETS,STARCH,ARABINOXYLANS}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{25}},
  title        = {{Impact of xylanase and glucanase on oligosaccharide formation, carbohydrate fermentation patterns, and nutrient utilization in the gastrointestinal tract of broilers}},
  url          = {{http://dx.doi.org/10.3390/ani11051285}},
  volume       = {{11}},
  year         = {{2021}},
}

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