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Pharmacological and genetic manipulations of Ca2+ signaling have contrasting effects on auxin-regulated trafficking

Ren Wang (UGent) , Ellie Himschoot (UGent) , Matteo Grenzi, Jian Chen (UGent) , Melanie Krebs, Karin Schumacher, Moritz Nowack (UGent) , Daniël Van Damme (UGent) , Ive De Smet (UGent) , Tom Beeckman (UGent) , et al.
(2021)
Author
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Abstract
A large part of a plants’ developmental plasticity relies on the activities of the phytohormone auxin and the regulation of its own distribution. This process involves a cohort of transcriptional and non-transcriptional effects of auxin on polar auxin transport, regulating the abundancy, biochemical activity and polar localization of the molecular components, predominantly PIN auxin exporters. While the transcriptional auxin signaling cascade has been well characterized, the mechanism and role of non-transcriptional auxin signaling remains largely elusive. Here, we addressed the potential involvement of auxin-induced Ca2+ signaling in auxin’s inhibitory effect on PIN endocytic trafficking. On the one hand, exogenous manipulations of Ca2+ availability and signaling effectively antagonized auxin effects suggesting that auxin-induced Ca2+ signaling is required for inhibition of internalization. On the other hand, we addressed the auxin-mediated inhibition of PIN internalization in the auxin signaling (tir1afb2,3) or Ca2+ channel (cngc14) mutants. These mutants were strongly defective in auxin-triggered Ca2+ signaling, but not in auxin-inhibited internalization. These data imply that, while Ca2+ signaling may be required for normal PIN trafficking, auxin-mediated increase in Ca2+ signaling is not a direct part of a downstream mechanism that mediates auxin effects on Brefeldin A-visualized PIN intercellular aggregation. These contrasting results obtained by comparing the mutant analysis versus the exogenous manipulations of Ca2+ availability and signaling illustrate the critical importance of genetics to unravel the role of Ca2+ in a process of interest.

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MLA
Wang, Ren, et al. Pharmacological and Genetic Manipulations of Ca2+ Signaling Have Contrasting Effects on Auxin-Regulated Trafficking. 2021, doi:10.1101/2020.09.04.283101.
APA
Wang, R., Himschoot, E., Grenzi, M., Chen, J., Krebs, M., Schumacher, K., … Vanneste, S. (2021). Pharmacological and genetic manipulations of Ca2+ signaling have contrasting effects on auxin-regulated trafficking. https://doi.org/10.1101/2020.09.04.283101
Chicago author-date
Wang, Ren, Ellie Himschoot, Matteo Grenzi, Jian Chen, Melanie Krebs, Karin Schumacher, Moritz Nowack, et al. 2021. “Pharmacological and Genetic Manipulations of Ca2+ Signaling Have Contrasting Effects on Auxin-Regulated Trafficking.” https://doi.org/10.1101/2020.09.04.283101.
Chicago author-date (all authors)
Wang, Ren, Ellie Himschoot, Matteo Grenzi, Jian Chen, Melanie Krebs, Karin Schumacher, Moritz Nowack, Daniël Van Damme, Ive De Smet, Tom Beeckman, Alex Costa, Jiří Friml, and Steffen Vanneste. 2021. “Pharmacological and Genetic Manipulations of Ca2+ Signaling Have Contrasting Effects on Auxin-Regulated Trafficking.” doi:10.1101/2020.09.04.283101.
Vancouver
1.
Wang R, Himschoot E, Grenzi M, Chen J, Krebs M, Schumacher K, et al. Pharmacological and genetic manipulations of Ca2+ signaling have contrasting effects on auxin-regulated trafficking. 2021.
IEEE
[1]
R. Wang et al., “Pharmacological and genetic manipulations of Ca2+ signaling have contrasting effects on auxin-regulated trafficking.” 2021.
@misc{8706353,
  abstract     = {{A large part of a plants’ developmental plasticity relies on the activities of the phytohormone auxin and the regulation of its own distribution. This process involves a cohort of transcriptional and non-transcriptional effects of auxin on polar auxin transport, regulating the abundancy, biochemical activity and polar localization of the molecular components, predominantly PIN auxin exporters. While the transcriptional auxin signaling cascade has been well characterized, the mechanism and role of non-transcriptional auxin signaling remains largely elusive. Here, we addressed the potential involvement of auxin-induced Ca2+ signaling in auxin’s inhibitory effect on PIN endocytic trafficking. On the one hand, exogenous manipulations of Ca2+ availability and signaling effectively antagonized auxin effects suggesting that auxin-induced Ca2+ signaling is required for inhibition of internalization. On the other hand, we addressed the auxin-mediated inhibition of PIN internalization in the auxin signaling (tir1afb2,3) or Ca2+ channel (cngc14) mutants. These mutants were strongly defective in auxin-triggered Ca2+ signaling, but not in auxin-inhibited internalization. These data imply that, while Ca2+ signaling may be required for normal PIN trafficking, auxin-mediated increase in Ca2+ signaling is not a direct part of a downstream mechanism that mediates auxin effects on Brefeldin A-visualized PIN intercellular aggregation. These contrasting results obtained by comparing the mutant analysis versus the exogenous manipulations of Ca2+ availability and signaling illustrate the critical importance of genetics to unravel the role of Ca2+ in a process of interest.}},
  author       = {{Wang, Ren and Himschoot, Ellie and Grenzi, Matteo and Chen, Jian and Krebs, Melanie and Schumacher, Karin and Nowack, Moritz and Van Damme, Daniël and De Smet, Ive and Beeckman, Tom and Costa, Alex and Friml, Jiří and Vanneste, Steffen}},
  language     = {{eng}},
  title        = {{Pharmacological and genetic manipulations of Ca2+ signaling have contrasting effects on auxin-regulated trafficking}},
  url          = {{http://dx.doi.org/10.1101/2020.09.04.283101}},
  year         = {{2021}},
}

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