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A comparison of the in vitro effects of 2'fucosyllactose and lactose on the composition and activity of gut microbiota from infants and toddlers

(2021) NUTRIENTS. 13(3).
Author
Organization
Abstract
Because of the recognized health benefits of breast milk, it is recommended as the sole nutrition source during the first 6 months of life. Among the bioactive components are human milk oligosaccharides (HMOs) that exert part of their activity via the gut microbiota. Here, we investigated the gut microbiota fermentation of HMO 2'fucosyllactose (2'-FL), using two in vitro models (48 h fecal incubations and the long-term mucosal simulator of the human intestinal microbial ecosystem [M-SHIME(R)]) with fecal samples from 3-month-old breastfed (BF) infants as well as 2-3 year old toddlers. The short-term model allowed the screening of five donors for each group and provided supportive data for the M-SHIME(R) study. A key finding was the strong and immediate increase in the relative abundance of Bifidobacteriaceae following 2'-FL fermentation by both the BF infant and toddler microbiota in the M-SHIME(R). At the metabolic level, while decreasing branched-chain fatty acids, 2'-FL strongly increased acetate production together with increases in the health-related propionate and butyrate whilst gas production only mildly increased. Notably, consistently lower gas production was observed with 2'-FL fermentation as compared to lactose, suggesting that reduced discomfort during the dynamic microbiome establishment in early life may be an advantage along with the bifidogenic effect observed.
Keywords
2'fucosyllactose, human milk oligosaccharides, infant gut microbiota, lactose, mucosal simulator of the human intestinal microbial ecosystem, prebiotic, toddler gut microbiota

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MLA
Van den Abbeele, Pieter, et al. “A Comparison of the in Vitro Effects of 2’fucosyllactose and Lactose on the Composition and Activity of Gut Microbiota from Infants and Toddlers.” NUTRIENTS, vol. 13, no. 3, 2021, doi:10.3390/nu13030726.
APA
Van den Abbeele, P., Sprenger, N., Ghyselinck, J., Marsaux, B., Marzorati, M., & Rochat, F. (2021). A comparison of the in vitro effects of 2’fucosyllactose and lactose on the composition and activity of gut microbiota from infants and toddlers. NUTRIENTS, 13(3). https://doi.org/10.3390/nu13030726
Chicago author-date
Van den Abbeele, Pieter, Norbert Sprenger, Jonas Ghyselinck, Benoit Marsaux, Massimo Marzorati, and Florence Rochat. 2021. “A Comparison of the in Vitro Effects of 2’fucosyllactose and Lactose on the Composition and Activity of Gut Microbiota from Infants and Toddlers.” NUTRIENTS 13 (3). https://doi.org/10.3390/nu13030726.
Chicago author-date (all authors)
Van den Abbeele, Pieter, Norbert Sprenger, Jonas Ghyselinck, Benoit Marsaux, Massimo Marzorati, and Florence Rochat. 2021. “A Comparison of the in Vitro Effects of 2’fucosyllactose and Lactose on the Composition and Activity of Gut Microbiota from Infants and Toddlers.” NUTRIENTS 13 (3). doi:10.3390/nu13030726.
Vancouver
1.
Van den Abbeele P, Sprenger N, Ghyselinck J, Marsaux B, Marzorati M, Rochat F. A comparison of the in vitro effects of 2’fucosyllactose and lactose on the composition and activity of gut microbiota from infants and toddlers. NUTRIENTS. 2021;13(3).
IEEE
[1]
P. Van den Abbeele, N. Sprenger, J. Ghyselinck, B. Marsaux, M. Marzorati, and F. Rochat, “A comparison of the in vitro effects of 2’fucosyllactose and lactose on the composition and activity of gut microbiota from infants and toddlers,” NUTRIENTS, vol. 13, no. 3, 2021.
@article{8704652,
  abstract     = {{Because of the recognized health benefits of breast milk, it is recommended as the sole nutrition source during the first 6 months of life. Among the bioactive components are human milk oligosaccharides (HMOs) that exert part of their activity via the gut microbiota. Here, we investigated the gut microbiota fermentation of HMO 2'fucosyllactose (2'-FL), using two in vitro models (48 h fecal incubations and the long-term mucosal simulator of the human intestinal microbial ecosystem [M-SHIME(R)]) with fecal samples from 3-month-old breastfed (BF) infants as well as 2-3 year old toddlers. The short-term model allowed the screening of five donors for each group and provided supportive data for the M-SHIME(R) study. A key finding was the strong and immediate increase in the relative abundance of Bifidobacteriaceae following 2'-FL fermentation by both the BF infant and toddler microbiota in the M-SHIME(R). At the metabolic level, while decreasing branched-chain fatty acids, 2'-FL strongly increased acetate production together with increases in the health-related propionate and butyrate whilst gas production only mildly increased. Notably, consistently lower gas production was observed with 2'-FL fermentation as compared to lactose, suggesting that reduced discomfort during the dynamic microbiome establishment in early life may be an advantage along with the bifidogenic effect observed.}},
  articleno    = {{726}},
  author       = {{Van den Abbeele, Pieter and Sprenger, Norbert and Ghyselinck, Jonas and Marsaux, Benoit and Marzorati, Massimo and Rochat, Florence}},
  issn         = {{2072-6643}},
  journal      = {{NUTRIENTS}},
  keywords     = {{2'fucosyllactose,human milk oligosaccharides,infant gut microbiota,lactose,mucosal simulator of the human intestinal microbial ecosystem,prebiotic,toddler gut microbiota}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{22}},
  title        = {{A comparison of the in vitro effects of 2'fucosyllactose and lactose on the composition and activity of gut microbiota from infants and toddlers}},
  url          = {{http://dx.doi.org/10.3390/nu13030726}},
  volume       = {{13}},
  year         = {{2021}},
}

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