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Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking

Madhumitha Narasimhan, Michelle Gallei, Shutang Tan, Alexander Johnson, Inge Verstraeten, Lanxin Li, Lesia Rodriguez, Huibin Han, Ellie Himschoot (UGent) , Ren Wang (UGent) , et al.
(2021) PLANT PHYSIOLOGY. 186(2). p.1122-1142
Author
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Abstract
The phytohormone auxin and its directional transport through tissues are intensively studied. However, a mechanistic understanding of auxin-mediated feedback on endocytosis and polar distribution of PIN auxin transporters remains limited due to contradictory observations and interpretations. Here, we used state-of-the-art methods to reexamine the auxin effects on PIN endocytic trafficking. We used high auxin concentrations or longer treatments versus lower concentrations and shorter treatments of natural (IAA) and synthetic (NAA) auxins to distinguish between specific and nonspecific effects. Longer treatments of both auxins interfere with Brefeldin A-mediated intracellular PIN2 accumulation and also with general aggregation of endomembrane compartments. NAA treatment decreased the internalization of the endocytic tracer dye, FM4-64; however, NAA treatment also affected the number, distribution, and compartment identity of the early endosome/trans-Golgi network (EE/TGN), rendering the FM4-64 endocytic assays at high NAA concentrations unreliable. To circumvent these nonspecific effects of NAA and IAA affecting the endomembrane system, we opted for alternative approaches visualizing the endocytic events directly at the plasma membrane (PM). Using Total Internal Reflection Fluorescence (TIRF) microscopy, we saw no significant effects of IAA or NAA treatments on the incidence and dynamics of clathrin foci, implying that these treatments do not affect the overall endocytosis rate. However, both NAA and IAA at low concentrations rapidly and specifically promoted endocytosis of photo-converted PIN2 from the PM. These analyses identify a specific effect of NAA and IAA on PIN2 endocytosis, thus contributing to its polarity maintenance and furthermore illustrate that high auxin levels have nonspecific effects on trafficking and endomembrane compartments.
Keywords
Plant Science, Genetics, Physiology, GNOM ARF-GEF, MEDIATES ENDOCYTOSIS, MEMBRANE TRAFFICKING, VACUOLAR TRAFFICKING, ARABIDOPSIS-THALIANA, PROTEIN TRAFFICKING, GOLGI-APPARATUS, BREFELDIN-A, PLANT, TRANSPORT

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MLA
Narasimhan, Madhumitha, et al. “Systematic Analysis of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” PLANT PHYSIOLOGY, vol. 186, no. 2, 2021, pp. 1122–42, doi:10.1093/plphys/kiab134.
APA
Narasimhan, M., Gallei, M., Tan, S., Johnson, A., Verstraeten, I., Li, L., … Friml, J. (2021). Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. PLANT PHYSIOLOGY, 186(2), 1122–1142. https://doi.org/10.1093/plphys/kiab134
Chicago author-date
Narasimhan, Madhumitha, Michelle Gallei, Shutang Tan, Alexander Johnson, Inge Verstraeten, Lanxin Li, Lesia Rodriguez, et al. 2021. “Systematic Analysis of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” PLANT PHYSIOLOGY 186 (2): 1122–42. https://doi.org/10.1093/plphys/kiab134.
Chicago author-date (all authors)
Narasimhan, Madhumitha, Michelle Gallei, Shutang Tan, Alexander Johnson, Inge Verstraeten, Lanxin Li, Lesia Rodriguez, Huibin Han, Ellie Himschoot, Ren Wang, Steffen Vanneste, Judit Sánchez-Simarro, Fernando Aniento, Maciek Adamowski, and Jiří Friml. 2021. “Systematic Analysis of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” PLANT PHYSIOLOGY 186 (2): 1122–1142. doi:10.1093/plphys/kiab134.
Vancouver
1.
Narasimhan M, Gallei M, Tan S, Johnson A, Verstraeten I, Li L, et al. Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. PLANT PHYSIOLOGY. 2021;186(2):1122–42.
IEEE
[1]
M. Narasimhan et al., “Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking,” PLANT PHYSIOLOGY, vol. 186, no. 2, pp. 1122–1142, 2021.
@article{8703764,
  abstract     = {{The phytohormone auxin and its directional transport through tissues are intensively studied. However, a mechanistic understanding of auxin-mediated feedback on endocytosis and polar distribution of PIN auxin transporters remains limited due to contradictory observations and interpretations. Here, we used state-of-the-art methods to reexamine the auxin effects on PIN endocytic trafficking. We used high auxin concentrations or longer treatments versus lower concentrations and shorter treatments of natural (IAA) and synthetic (NAA) auxins to distinguish between specific and nonspecific effects. Longer treatments of both auxins interfere with Brefeldin A-mediated intracellular PIN2 accumulation and also with general aggregation of endomembrane compartments. NAA treatment decreased the internalization of the endocytic tracer dye, FM4-64; however, NAA treatment also affected the number, distribution, and compartment identity of the early endosome/trans-Golgi network (EE/TGN), rendering the FM4-64 endocytic assays at high NAA concentrations unreliable. To circumvent these nonspecific effects of NAA and IAA affecting the endomembrane system, we opted for alternative approaches visualizing the endocytic events directly at the plasma membrane (PM). Using Total Internal Reflection Fluorescence (TIRF) microscopy, we saw no significant effects of IAA or NAA treatments on the incidence and dynamics of clathrin foci, implying that these treatments do not affect the overall endocytosis rate. However, both NAA and IAA at low concentrations rapidly and specifically promoted endocytosis of photo-converted PIN2 from the PM. These analyses identify a specific effect of NAA and IAA on PIN2 endocytosis, thus contributing to its polarity maintenance and furthermore illustrate that high auxin levels have nonspecific effects on trafficking and endomembrane compartments.}},
  author       = {{Narasimhan, Madhumitha and Gallei, Michelle and Tan, Shutang and Johnson, Alexander and Verstraeten, Inge and Li, Lanxin and Rodriguez, Lesia and Han, Huibin and Himschoot, Ellie and Wang, Ren and Vanneste, Steffen and Sánchez-Simarro, Judit and Aniento, Fernando and Adamowski, Maciek and Friml, Jiří}},
  issn         = {{0032-0889}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{Plant Science,Genetics,Physiology,GNOM ARF-GEF,MEDIATES ENDOCYTOSIS,MEMBRANE TRAFFICKING,VACUOLAR TRAFFICKING,ARABIDOPSIS-THALIANA,PROTEIN TRAFFICKING,GOLGI-APPARATUS,BREFELDIN-A,PLANT,TRANSPORT}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{1122--1142}},
  title        = {{Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking}},
  url          = {{http://dx.doi.org/10.1093/plphys/kiab134}},
  volume       = {{186}},
  year         = {{2021}},
}
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