Marine seagrass extract of Thalassia testudinum suppresses colorectal tumor growth, motility and angiogenesis by autophagic stress and immunogenic cell death pathways
- Author
- Ivones Hernández-Balmaseda, Idania Rodeiro Guerra, Ken Declerck, José Alfredo Herrera Isidrón, Claudina Pérez-Novo, Guy Van Camp, Olivier De Wever (UGent) , Kethia González, Mayrel Labrada, Adriana Carr, Geovanni Dantas-Cassali, Diego Carlos dos Reis, Livan Delgado-Roche, Roberto Rafael Nuñez, René Delgado-Hernández, Miguel David Fernández, Miriam T. Paz-Lopes and Wim Vanden Berghe (UGent)
- Organization
- Abstract
- Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm Thalassia testudinum (TTE) in colon tumor cell lines (RKO, SW480, and CT26) and a syngeneic allograft murine colorectal cancer model. MTT assays revealed a dose-dependent decrease of cell viability of RKO, CT26, and SW480 cells upon TTE treatment with IC50 values of, respectively, 175, 115, and 60 mu g/mL. Furthermore, TTE significantly prevented basal and bFGF-induced angiogenesis in the chicken chorioallantoic membrane angiogenesis assay. In addition, TTE suppressed bFGF-induced migration of endothelial cells in a wound closure assay. Finally, TTE treatment abrogated CT26 colorectal cancer growth and increased overall organism survival in a syngeneic murine allograft model. Corresponding transcriptome profiling and pathway analysis allowed for the identification of the mechanism of action for the antitumor effects of TTE. In line with our in vitro/in vivo results, TTE treatment triggers ATF4-P53-NF kappa B specific gene expression and autophagy stress pathways. This results in suppression of colon cancer cell growth, cell motility, and angiogenesis pathways in vitro and in addition promotes antitumor immunogenic cell death in vivo.
- Keywords
- Drug Discovery, Thalassia testudinum, cytotoxicity, antitumor, anti-angiogenic, gene expression
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8703478
- MLA
- Hernández-Balmaseda, Ivones, et al. “Marine Seagrass Extract of Thalassia Testudinum Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways.” MARINE DRUGS, vol. 19, no. 2, 2021, doi:10.3390/md19020052.
- APA
- Hernández-Balmaseda, I., Guerra, I. R., Declerck, K., Herrera Isidrón, J. A., Pérez-Novo, C., Van Camp, G., … Vanden Berghe, W. (2021). Marine seagrass extract of Thalassia testudinum suppresses colorectal tumor growth, motility and angiogenesis by autophagic stress and immunogenic cell death pathways. MARINE DRUGS, 19(2). https://doi.org/10.3390/md19020052
- Chicago author-date
- Hernández-Balmaseda, Ivones, Idania Rodeiro Guerra, Ken Declerck, José Alfredo Herrera Isidrón, Claudina Pérez-Novo, Guy Van Camp, Olivier De Wever, et al. 2021. “Marine Seagrass Extract of Thalassia Testudinum Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways.” MARINE DRUGS 19 (2). https://doi.org/10.3390/md19020052.
- Chicago author-date (all authors)
- Hernández-Balmaseda, Ivones, Idania Rodeiro Guerra, Ken Declerck, José Alfredo Herrera Isidrón, Claudina Pérez-Novo, Guy Van Camp, Olivier De Wever, Kethia González, Mayrel Labrada, Adriana Carr, Geovanni Dantas-Cassali, Diego Carlos dos Reis, Livan Delgado-Roche, Roberto Rafael Nuñez, René Delgado-Hernández, Miguel David Fernández, Miriam T. Paz-Lopes, and Wim Vanden Berghe. 2021. “Marine Seagrass Extract of Thalassia Testudinum Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways.” MARINE DRUGS 19 (2). doi:10.3390/md19020052.
- Vancouver
- 1.Hernández-Balmaseda I, Guerra IR, Declerck K, Herrera Isidrón JA, Pérez-Novo C, Van Camp G, et al. Marine seagrass extract of Thalassia testudinum suppresses colorectal tumor growth, motility and angiogenesis by autophagic stress and immunogenic cell death pathways. MARINE DRUGS. 2021;19(2).
- IEEE
- [1]I. Hernández-Balmaseda et al., “Marine seagrass extract of Thalassia testudinum suppresses colorectal tumor growth, motility and angiogenesis by autophagic stress and immunogenic cell death pathways,” MARINE DRUGS, vol. 19, no. 2, 2021.
@article{8703478,
abstract = {{Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm Thalassia testudinum (TTE) in colon tumor cell lines (RKO, SW480, and CT26) and a syngeneic allograft murine colorectal cancer model. MTT assays revealed a dose-dependent decrease of cell viability of RKO, CT26, and SW480 cells upon TTE treatment with IC50 values of, respectively, 175, 115, and 60 mu g/mL. Furthermore, TTE significantly prevented basal and bFGF-induced angiogenesis in the chicken chorioallantoic membrane angiogenesis assay. In addition, TTE suppressed bFGF-induced migration of endothelial cells in a wound closure assay. Finally, TTE treatment abrogated CT26 colorectal cancer growth and increased overall organism survival in a syngeneic murine allograft model. Corresponding transcriptome profiling and pathway analysis allowed for the identification of the mechanism of action for the antitumor effects of TTE. In line with our in vitro/in vivo results, TTE treatment triggers ATF4-P53-NF kappa B specific gene expression and autophagy stress pathways. This results in suppression of colon cancer cell growth, cell motility, and angiogenesis pathways in vitro and in addition promotes antitumor immunogenic cell death in vivo.}},
articleno = {{52}},
author = {{Hernández-Balmaseda, Ivones and Guerra, Idania Rodeiro and Declerck, Ken and Herrera Isidrón, José Alfredo and Pérez-Novo, Claudina and Van Camp, Guy and De Wever, Olivier and González, Kethia and Labrada, Mayrel and Carr, Adriana and Dantas-Cassali, Geovanni and dos Reis, Diego Carlos and Delgado-Roche, Livan and Nuñez, Roberto Rafael and Delgado-Hernández, René and Fernández, Miguel David and Paz-Lopes, Miriam T. and Vanden Berghe, Wim}},
issn = {{1660-3397}},
journal = {{MARINE DRUGS}},
keywords = {{Drug Discovery,Thalassia testudinum,cytotoxicity,antitumor,anti-angiogenic,gene expression}},
language = {{eng}},
number = {{2}},
pages = {{17}},
title = {{Marine seagrass extract of Thalassia testudinum suppresses colorectal tumor growth, motility and angiogenesis by autophagic stress and immunogenic cell death pathways}},
url = {{http://doi.org/10.3390/md19020052}},
volume = {{19}},
year = {{2021}},
}
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