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Method for beta-carotene extraction from processed baby foods as a model for plant-based fatty food products

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Abstract
Reliable quantitative determination of carotenoids in complex food matrices such as processed baby food products is challenging because of their incorporation in rigid cellular structures, their sensitivity to oxidation and their lipophilic character. A one-pot liquid-liquid beta-carotene extraction procedure is described for solid baby foods, in the presence of enzymes (Clara-Diastase and Rapidase) facilitating matrix disintegration. The combined extraction and enzymatic dissolution not only protected beta-carotene from oxidation compared to the sequential approach, but also reduced the use of solvents and amount of filtrations steps, favouring a higher recovery. The addition of phenolic antioxidants (BHT, TBHQ and BHA) to calibration solutions and during the procedure at 25 mg/mL resulted in an up to 2.5-fold higher absorbance of beta-carotene solutions which was not observed for trans-beta-apo-8 '-carotenal (used as internal standard) solutions. When applying the full procedure on beta-carotene spiked sunflower oil, an apparent recovery of 80% for beta-carotene was obtained. Finally, this protocol was applied to 50 vegetable-based and 22 fruit-based processed baby foods (range 0 to 1179 and 504 mu g/100 g, respectively), and it was concluded that this extraction procedure may be used for similar processed foods products. The procedure proved to be sensitive (LOD = 0.12 mu g/mL) and reproducible (CV for baby foods: 4-10%).
Keywords
Food Science, Carotenoid analysis, Baby foods, Plant-based fatty products, Antioxidant, BIOAVAILABILITY, OXIDATION

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MLA
Grootaert, Charlotte, et al. “Method for Beta-Carotene Extraction from Processed Baby Foods as a Model for Plant-Based Fatty Food Products.” FOOD RESEARCH INTERNATIONAL, vol. 144, 2021, doi:10.1016/j.foodres.2021.110332.
APA
Grootaert, C., Vansteenland, M., Vandemoortele, A., Van Camp, J., & De Meulenaer, B. (2021). Method for beta-carotene extraction from processed baby foods as a model for plant-based fatty food products. FOOD RESEARCH INTERNATIONAL, 144. https://doi.org/10.1016/j.foodres.2021.110332
Chicago author-date
Grootaert, Charlotte, Margot Vansteenland, Angelique Vandemoortele, John Van Camp, and Bruno De Meulenaer. 2021. “Method for Beta-Carotene Extraction from Processed Baby Foods as a Model for Plant-Based Fatty Food Products.” FOOD RESEARCH INTERNATIONAL 144. https://doi.org/10.1016/j.foodres.2021.110332.
Chicago author-date (all authors)
Grootaert, Charlotte, Margot Vansteenland, Angelique Vandemoortele, John Van Camp, and Bruno De Meulenaer. 2021. “Method for Beta-Carotene Extraction from Processed Baby Foods as a Model for Plant-Based Fatty Food Products.” FOOD RESEARCH INTERNATIONAL 144. doi:10.1016/j.foodres.2021.110332.
Vancouver
1.
Grootaert C, Vansteenland M, Vandemoortele A, Van Camp J, De Meulenaer B. Method for beta-carotene extraction from processed baby foods as a model for plant-based fatty food products. FOOD RESEARCH INTERNATIONAL. 2021;144.
IEEE
[1]
C. Grootaert, M. Vansteenland, A. Vandemoortele, J. Van Camp, and B. De Meulenaer, “Method for beta-carotene extraction from processed baby foods as a model for plant-based fatty food products,” FOOD RESEARCH INTERNATIONAL, vol. 144, 2021.
@article{8701234,
  abstract     = {{Reliable quantitative determination of carotenoids in complex food matrices such as processed baby food products is challenging because of their incorporation in rigid cellular structures, their sensitivity to oxidation and their lipophilic character. A one-pot liquid-liquid beta-carotene extraction procedure is described for solid baby foods, in the presence of enzymes (Clara-Diastase and Rapidase) facilitating matrix disintegration. The combined extraction and enzymatic dissolution not only protected beta-carotene from oxidation compared to the sequential approach, but also reduced the use of solvents and amount of filtrations steps, favouring a higher recovery. The addition of phenolic antioxidants (BHT, TBHQ and BHA) to calibration solutions and during the procedure at 25 mg/mL resulted in an up to 2.5-fold higher absorbance of beta-carotene solutions which was not observed for trans-beta-apo-8 '-carotenal (used as internal standard) solutions. When applying the full procedure on beta-carotene spiked sunflower oil, an apparent recovery of 80% for beta-carotene was obtained. Finally, this protocol was applied to 50 vegetable-based and 22 fruit-based processed baby foods (range 0 to 1179 and 504 mu g/100 g, respectively), and it was concluded that this extraction procedure may be used for similar processed foods products. The procedure proved to be sensitive (LOD = 0.12 mu g/mL) and reproducible (CV for baby foods: 4-10%).}},
  articleno    = {{110332}},
  author       = {{Grootaert, Charlotte and Vansteenland, Margot and Vandemoortele, Angelique and Van Camp, John and De Meulenaer, Bruno}},
  issn         = {{0963-9969}},
  journal      = {{FOOD RESEARCH INTERNATIONAL}},
  keywords     = {{Food Science,Carotenoid analysis,Baby foods,Plant-based fatty products,Antioxidant,BIOAVAILABILITY,OXIDATION}},
  language     = {{eng}},
  pages        = {{9}},
  title        = {{Method for beta-carotene extraction from processed baby foods as a model for plant-based fatty food products}},
  url          = {{http://dx.doi.org/10.1016/j.foodres.2021.110332}},
  volume       = {{144}},
  year         = {{2021}},
}

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